T cells from an HLA-DR11/DR12 responder were stimulated in mixed lymphocyte culture with carrying the DR1 antigen. After priming, proliferated response to both DR1-positive-stimulating and a peptide derived polymorphic region of HLA-DR beta 1*0101 chain presented by responder's antigen-presenting (APC). The dominant epitope recognized primed corresponded residue 21-42 was HLA-DR12 peptide-reactive express cell receptor V 3. results demonstrate that allopeptides processing presentation donor...

Rab GTPases represent the largest subfamily of Ras-related small and regulate membrane trafficking. Vesicular transport is a general mechanism that governs intracellular trafficking along endocytic exocytic pathways in all eukaryotic cells. Fusarium graminearum filamentous fungus causes devastating economically important head blight wheat related species. The vesicular not well understood, little known about F. graminearum. In this study, we systematically characterized eleven FgRabs by live...

Heat stress severely affects the annual agricultural production. transcription factors (HSFs) represent a critical regulatory juncture in heat response (HSR) of plants. The HsfA1-dependent pathway has been explored well, but mechanism HsfA1-independent is still under-investigated. In present research, HsfA4, an important gene pathway, was isolated from lilies (Lilium longiflorum) using RACE method, which encodes 435 amino acids. LlHsfA4 contains typical domain HSFs and belongs to HSF A4...

High temperature seriously limits the annual production of fresh cut lilies, which is one four major flowers in global flower market. There were few transcriptomes focused on gene expression lilies under heat stress. In order to reveal potential response patterns bulbous plants and provide important genes for further genetic engineering techniques improve thermotolerance lily, RNA sequencing treatments conducted.In this study, seedlings Lilium longiflorum 'White Heaven' heat-treated at 37 °C...

Based on studies of monocot crops and eudicot model plants, the DREB2 class AP2-type transcription factors have been shown to play crucial roles in various abiotic stresses, especially upstream heat stress response; however, research DREB2s has not reported non-gramineous plants. Here, we identified a novel (LlDREB2B) from lily (Lilium longiflorum), which was homologous AtDREB2A Arabidopsis, OsDREB2B rice, ZmDREB2A maize. LlDREB2B induced by heat, cold, salt, mannitol stress, its protein had...

Background: Stomach adenocarcinoma (STAD) presents a challenge given its advanced stage at diagnosis and poor prognosis. Integrin subunit alpha 11 (ITGA11) encodes integrin has been implicated in promoting tumorigenesis development by participating cell proliferation invasion. However, the precise mechanism of ITGA11 STAD remains unclear. Methods: The differences expression levels between 375 gastric cancer samples 32 paracancerous tissue from Cancer Genome Atlas (TCGA) database were...

Land plants alternate between asexual sporophytes and sexual gametophytes. Unlike seed plants, ferns develop free-living Gametophytes of the model fern Ceratopteris exhibit two sex types: hermaphrodites with pluripotent meristems males lacking meristems. In absence pheromone antheridiogen, convert to by forming de novo meristems, though mechanisms remain unclear. Using long-term time-lapse imaging computational analyses, we captured male-to-hermaphrodite conversion at single-cell resolution...

LYSET is a recently identified Golgi transmembrane (TM) protein, and inactivating mutations in the gene phenocopy mucolipidosis II (MLII), lysosomal storage disease caused by loss of function GlcNAc-1-phosphotransferase αβ (GNPTαβ), which tags hydrolases with mannose 6-phosphate (M6P) tag for delivery to lysosomes. It conceivable that facilitates integration both hydrophilic TM helices (TMHs) GNPTαβ retain latter Golgi, although this has only been directly demonstrated N-terminal TMH wherein...

Aging of the fetal membranes participates in labor onset. However, underlying mechanism is poorly understood. Here, we identify that classical secretory protein S100 calcium-binding A9 (S100A9), upon de-phosphorylation at Thr 113, translocates to nuclei amnion fibroblasts human membranes, where S100A9 causes heterochromatin erosion via segregation maintenance proteins, resulting Long Interspersed Nuclear Element-1 (LINE1) de-repression parturition. Increased LINE1 retrotransposition further...

T cell reactivity to alloantigens results from direct and indirect recognition of allogeneic MHC molecules and/or peptides. Although is not self-MHC restricted, recognition, the result alloantigen processing presentation by host APC, restricted self (responder) molecule which allopeptide has bound. We have studied restriction TCR usage in alloreactivity a synthetic peptide corresponding amino acid residues 21-42 DR beta 1*0101 molecule. lines were developed vitro immunization cells three...

This study aimed to investigate the effect of oil processing technologies on sensory qualities sesame oils and identify drivers liking. Using a check-all-that-apply (CATA) question hedonic scale, 150 consumers evaluated acceptability characteristics 5 samples including an aqueous extracted (S1), cold-pressed (S2), two batches screw-pressed (S3 S5) one crude (S4). Solid-phase microextraction-gas chromatography-olfactometry-mass spectrometry (SPME-GC-O-MS) was used odour-active compounds. The...

In contrast to seed plants, the gametophytes of seed-free plants develop pluripotent meristems, which promote and sustain their independent growth development. To date, cellular basis meristem development in ferns remains largely unknown. this study, we used Woodsia obtusa, blunt-lobe cliff fern, quantitatively determine cell dynamics two different types apical meristems - initial centered multicellular gametophytes. Through confocal time-lapse live imaging computational image analysis...

It has been suggested that self major histocompatibility complex (MHC) peptides bound to MHC molecules may be involved in the intrathymic induction of tolerance. We studied antigenicity synthetic derived from first domain DR beta 1*0101 chain a responder. found peptide corresponding residues 21-42 could elicit proliferation autoreactive T cells. A cell line (TCL-SUN) and 7 9 clones (TCC) TCL-SUN specifically recognized presence APCs carrying allele. positive stimulated TCCs absence peptide,...

Summary Vps17 is a sorting nexin (SNX) and component of the retromer, protein complex mediating retrograde vesicle transport between endosomes trans‐Golgi network. However, its role in development pathogenicity filamentous fungi such as rice blast fungus ( Magnaporthe oryzae ) remains unclear. We investigate functional relationship SNX cargo‐selective (CSC) fungal retromer by genetic analysis, live cell imaging immunological assay. Our data show that MoVps17 null mutation causes defects...

Abstract Background Epidemiological evidence between the sleep disorders and allergy-related outcomes is limited. Objectives The purpose of present study was to estimate relationship in adults. Methods We built logistic regression models examine associations adult participants using 2005–2006 NHANES database. Allergy-related included sIgE levels, asthma, hay fever, sneezing, wheezing, eczema. Sleep latency, length, problems, OSA symptoms, daytime sleepiness. A t-test used for between-group...

Lily is an important cut flower all over the world and enjoys cool environments. The high summer temperature in China affects quality quantity of lily–flower production. In this study, heat stress transcription factor HsfA2 was cloned from two cultivated commercial varieties, <italic>Lilium longiflorum</italic> 'White Heaven' <italic>Lilium</italic> oriental 'Siberia,' which have different responses to stress. An actin-interacting protein 3 (AIP3) domain found LlHsfA2 but did not appear...

Lily (Lilium spp. and hybrids) is an important cut flower crop worldwide. flowers have large anthers, which release a amount of pollen that stains the tepals or clothing thus can affect commercial value flowers. In this study, lily Oriental 'Siberia' was used to investigate regulatory mechanism anther development, may provide information prevent pollution in future. Based on bud length, length color, anatomical observations, development categorized into five stages: green (G),...

Objective The aim of the study was to determine whether proportion positive high-risk human papillomavirus (HR-HPV) tests in endocervical specimens transported dry differs from paired liquid media. Methods Five hundred women aged 30 55 years were recruited, Shanxi Bethune Hospital, China. Two samples collected endocervix per patient, one placed into empty vial, other a transport solution. All analyzed by AmpFire HR-HPV assay. Results Total 1,000 500 patients total invalid rate 0.2%...

To develop and evaluate the effectiveness/clinical application of an eosinophil cationic protein-myeloperoxidase (ECP-MPO) test paper before after treatment in patients with allergic rhinitis (AR).We included 40 controls 106 AR who were enrolled Allergy Clinic Renmin Hospital Wuhan University. Total IgE, specific IgE skin prick (SPT) detected all participants. treated oral cetirizine hydrochloride for 14 days. The ECP-MPO results, nasal secretion smear counts, rhinoconjunctivitis total...

Intervertebral disc degeneration (IVDD) is the initiating factor of adult degenerative scoliosis (ADS), and ADS further accelerates IVDD, creating a vicious cycle. Nevertheless, role Wnt/β-Catenin pathway in combined with IVDD (ADS-IVDD) remains mystery. Accordingly, this study was proposed to investigate effect axial stress on nucleus pulposus cells (NPCs) isolated from DS-IVDD patients.Normal NPCs (N-NPC) were purchased young (25-30 years; Y-NPC) old (65-70 O-NPC) ADS-IVDD patients primary...