In clinical practice, the emergence of ST11-K64 carbapenem-resistant Klebsiella pneumoniae (ST11-K64 CRKP) has become increasingly alarming. Despite this trend, limited research been conducted to elucidate and molecular characteristics these strains. This study aimed comprehensively investigate characteristics, antimicrobial resistance patterns, virulence-associated genes, epidemiology CRKP in Southwest China. A retrospective analysis was performed on patients infected with (CRKP) a tertiary...

Although HsfA3 (heat-stress transcription factor A3) is well characterized in heat stress, its roles other abiotic stresses are less clear. In this study, we isolated two homologous genes, LlHsfA3A and LlHsfA3B, from lily (Lilium longiflorum). Both genes were induced by but not salt stress. Overexpressing Arabidopsis enhanced basal acquired thermotolerance, while overexpressing LlHsfA3B just thermotolerance. both cases, plants showed hypersensitivity to a lack of sucrose exacerbated...

Heat stress transcription factors (HSFs) are central regulators of plant responses to heat stress. Their heat-induced transcriptional regulation has been extensively studied; however, their posttranscriptional and posttranslational is poorly understood. In a previous study, we established that there were at least two

Lily bulbils, which serve as advantageous axillary organs for vegetative propagation, have not been extensively studied in terms of the mechanism bulbil initiation. The functions auxin and sucrose metabolism implicated organ development, but their relationship regulating initiation remains unclear. In this study, exogenous indole-3-acetic acid (IAA) treatment increased endogenous levels at leaf axils significantly decreased number, whereas with polar transport inhibitor...

Based on studies of monocot crops and eudicot model plants, the DREB2 class AP2-type transcription factors have been shown to play crucial roles in various abiotic stresses, especially upstream heat stress response; however, research DREB2s has not reported non-gramineous plants. Here, we identified a novel (LlDREB2B) from lily (Lilium longiflorum), which was homologous AtDREB2A Arabidopsis, OsDREB2B rice, ZmDREB2A maize. LlDREB2B induced by heat, cold, salt, mannitol stress, its protein had...

As a lipophilic flavonol, quercetin has low bioavailability, which limits its application in foods. This work aimed to prepare hordein-based system deliver quercetin. We constructed hordein–whey isolate protein fibril (WPIF) complexes (H-Ws) by anti-solvent precipitation method at pH 2.5. The TEM results of the showed that spherical-like hordein particles were wrapped WPIF clusters form an interconnected network structure. FTIR spectra revealed hydrogen bonds and hydrophobic interactions...

Abstract Understanding corm development in flower bulbs is of importance for securing the quality cut flowers and propagation commercial stocks. Gladiolus one most popular bulb plants worldwide. Its characterized by starch accumulation. Previous research has shown that phytohormones (especially gibberellin (GA)) are involved tuber development. However, relationship between abscisic acid (ABA)/GA during remains unclear. To gain deeper insights into biological process development, we performed...

Abstract Plant architecture is defined by fates and positions of meristematic tissues has direct consequences on yield potential environmental adaptation the plant. In strawberries (Fragaria vesca L. F. × ananassa Duch.), shoot apical meristems can remain vegetative or differentiate into a terminal inflorescence meristem. Strawberry axillary buds (AXBs) are located in leaf axils either dormant follow one two possible developmental fates. AXBs develop stolons needed for clonal reproduction...

A runner, as an elongated branch, develops from the axillary bud (AXB) in leaf axil and is crucial for clonal propagation of cultivated strawberry (Fragaria × ananassa Duch.). Runner formation occurs at least two steps: AXB initiation outgrowth. HANABA TARANU (HAN ) encodes a GATA transcription factor that affects Arabidopsis promotes branching grass species, but underlying mechanism largely unknown. Here, function HAN homolog FaHAN runner was characterized. transcripts can be detected...

Dormancy is one of the least understood phenomena in plant biology; however, bud/corm dormancy an important economic trait agricultural/horticultural breeding. In this study, we isolated ABA biosynthesis gene, GhNCED, from transcriptome database corm release (CDR), and characterized its negative role regulating CDR. To understand transcriptional regulation yeast one-hybrid screening was conducted GhTCP19 identified shown to regulate GhNCED expression directly. An planta assay showed that...

Corm dormancy is an important trait for breeding in many bulb flowers, including the most cultivated Gladiolus hybridus. corms are modified underground stems that function as storage organs and remain dormant to survive adverse environmental conditions. Unlike seed dormancy, not much known about corm dormancy. Here, we characterize mechanism of release (CDR) Gladiolus. We identified ABA (abscisic acid) signaling regulator, GhPP2C1 (protein phosphatase 2C1), by transcriptome analysis CDR....

The exocyst, an evolutionarily conserved octameric protein complex, mediates tethering of vesicles to the plasma membrane in early stage exocytosis. Arabidopsis Exo70, a subunit exocyst has been found be involved plant immunity. Here, we characterize function OsExo70B1 rice. mainly expresses leaf and shoot its expression is induced by pathogen-associated molecular patterns (PAMPs) rice blast fungus Magnaporthe oryzae (M. oryzae). Knocking out results significantly decreased resistance...

The CCCH proteins play important roles in plant growth and development, hormone response, pathogen defense abiotic stress tolerance. However, the knowledge of their thermotolerance are scarce. Here, we identified a heat-inducible gene LlC3H18 from lily. was localized cytoplasm nucleus under normal conditions, while it translocated cytoplasmic foci co-located with markers two messenger ribonucleoprotein (mRNP) granules, processing bodies (PBs) granules (SGs) heat also exhibited RNA-binding...

Lilies are bulbous flowers cherished for their aesthetic and medicinal value. Within the realm of lily cultivation, scale-cutting serves as a prevailing method vegetative propagation, yielding diminutive bulbs termed bulblets at base scales. However, burgeoning demand lilies necessitates optimization propagation strategies to satisfy surging requisites. This study centers on two synthetic cytokinin analogs, N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU) 2-isopentenyladenine (2-iP), identified...

Abstract Apple necrotic mosaic virus (ApNMV) is associated with apple disease in China. However, the mechanisms of ApNMV infection, as well host defence against virus, are still poorly understood. Mitochondrial ATP synthase plays a fundamental role regulation plant growth and development. mitochondrial function response to infection remains be defined. In present study, yeast two-hybrid (Y2H) screening revealed that oligomycin sensitivity-conferring protein (OSCP) subunit (MdATPO) interacts...

Abstract Bud dormancy is an important trait in geophytes that largely affects their flowering process and vegetative growth after release. Compared with seed dormancy, the regulation of bud still unclear. Abscisic acid (ABA) acts as predominant hormone regulates whole process. In Gladiolus (Gladiolus hybridus), cold storage promotes corm release (CDR) by repressing ABA biosynthesis signaling. However, mechanisms governing ABA-related processes during CDR via epigenetics are poorly...

The quality and yields of strawberry plants are seriously affected by abiotic stress every year. NAC (NAM, ATAF, CUC) transcription factors plant-specific, having various functions in plant development response to stress. In our study, FaNAC2 from (Fragaria × ananassa, cultivar "Benihoppe") was isolated found be a member the ATAF sub-family, belonging family factors. strongly expressed shoot apical meristem older leaves strawberries, induced cold, high salinity, drought To investigate how...

Abstract Gladiolus hybridus is one of the most popular flowers worldwide. However, its corm dormancy characteristic largely limits off‐season production. Long‐term cold treatment (LT), which increases sugar content and reduces abscisic acid (ABA), an efficient approach to accelerate release (CDR). Here, we identified a GhbZIP30‐ GhCCCH17 module that mediates antagonism between sugars ABA during CDR. We showed promoted CDR by reducing levels in Gladiolus. Our data demonstrated GhbZIP30...

SUMMARY Basic helix–loop–helix (bHLH) proteins comprise one of the largest families transcription factors in plants, which play roles plant development, secondary metabolism, and response to biotic/abiotic stresses. However, bHLH thermotolerance are largely unknown. Herein, we identified a heat‐inducible member family lily ( Lilium longiflorum ), named LlbHLH87, plays role thermotolerance. LlbHLH8 7 was rapidly induced by transient heat stress, its encoded protein localized nucleus,...

Lily bulbils, advantageous axillary organs used for asexual reproduction, have an underexplored developmental mechanism. Gibberellins are known to participate in bulbil development, but the regulatory mechanisms remain unclear. In this study, exogenous gibberellin (GA

The initiation and quality of flowering directly affect the time to market economic benefit cultivated strawberries, but underlying mechanisms these processes are largely unknown. To investigate gene activity during key period floral induction in time-course transcriptome analysis was performed on shoot apex strawberry cultivar 'Benihoppe.' A total 7177 differentially expressed genes (DEGs) were identified through pairwise comparisons. These DEGs grouped into four clusters with dynamic...