A5040213644- Entomopathogenic Microorganisms in Pest Control
- Plant Molecular Biology Research
- Insect symbiosis and bacterial influences
- Plant Gene Expression Analysis
- Insect Resistance and Genetics
- Plant Stress Responses and Tolerance
- Plant biochemistry and biosynthesis
- Neurogenetic and Muscular Disorders Research
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Nematode management and characterization studies
- RNA modifications and cancer
- Plant Virus Research Studies
- Plant Physiology and Cultivation Studies
- Immunotherapy and Immune Responses
- Fungal Biology and Applications
- Plant tissue culture and regeneration
- Insect-Plant Interactions and Control
- Plant Reproductive Biology
- Milk Quality and Mastitis in Dairy Cows
- Plant-Microbe Interactions and Immunity
- Insect Utilization and Effects
- Plant responses to water stress
- Hereditary Neurological Disorders
- Chromosomal and Genetic Variations
Chongqing University
2024-2025
Southwest University
2015-2025
Gansu University of Traditional Chinese Medicine
2025
Chaohu Hospital of Anhui Medical University
2025
Capital Normal University
2012-2024
Guangxi Maternal and Child Health Hospital
2024
Chongqing Academy of Agricultural Sciences
2024
University College London
2024
Southwest University of Science and Technology
2023-2024
PTC Therapeutics (United States)
2012-2023
SMA is an inherited disease that leads to loss of motor function and ambulation a reduced life expectancy. We have been working develop orally administrated, systemically distributed small molecules increase levels functional SMN protein. Compound 2 was the first SMN2 splicing modifier tested in clinical trials healthy volunteers patients. It safe well tolerated increased protein up 2-fold Nevertheless, its development stopped as precautionary measure because retinal toxicity observed...
Producing maternal haploids via a male inducer can greatly accelerate maize (Zea mays) breeding process. However, the mechanism underlying haploid formation remains unclear. In this study, we constructed two lines containing cytogenetic marker B chromosome or alien centromeric histone H3 variant-yellow fluorescent protein vector to investigate mechanism. The as pollinators were crossed with hybrid ZhengDan958. chromosomes detected in F1 at low frequency, which was direct evidence support...
Abstract Some R2R3 MYB transcription factors have been shown to be major regulators of phenylpropanoid biosynthetic pathway and impact secondary wall formation in plants. In this study, we describe the functional characterization PtoMYB156 , encoding a R2R3-MYB factor, from Populus tomentosa. Expression pattern analysis showed that is widely expressed all tissues examined, but predominantly leaves developing wood cells. localized nucleus acted as transcriptional repressor. Overexpression...
Spinal muscular atrophy (SMA) is the leading genetic cause of infant and toddler mortality, there currently no approved therapy available. SMA caused by mutation or deletion survival motor neuron 1 (SMN1) gene. These mutations deletions result in low levels functional SMN protein. SMN2, a paralogous gene to SMN1, undergoes alternative splicing exclusion exon 7, producing an unstable, truncated SMNΔ7 Herein, we report identification pyridopyrimidinone series small molecules that modify...
Abstract WRKY proteins are one of the largest transcription factor families in higher plants and play diverse roles various biological processes. Previous studies have shown that some members act as negative regulators secondary cell wall formation pith parenchyma cells. However, regulatory mechanism tree species remains largely unknown. In this study, PtrWRKY19 encoding a homolog Arabidopsis WRKY12 was isolated from Populus trichocarpa. expressed all tissues tested, with highest expression...
Drought is an environmental factor that can severely influence plant development and distribution, greatly affect the yield economic value of crops. We characterized CmBBX19, a BBX family subgroup IV member gene, from transcriptome database Chrysanthemum morifolium in response to drought stress. stress ABA treatments downregulated expression CmBBX19. generated CmBBX19-overexpressing (CmBBX19-OX) lines CmBBX19-suppressing (CmBBX19-RNAi), found suppressed CmBBX19 led enhanced tolerance...
Fungal insect pathogens have evolved diverse mechanisms to evade host immune recognition and defense responses. However, identification of fungal factors involved in evasion during cuticular penetration subsequent hemocoel colonization remains limited. Here, we report that the entomopathogenic fungus Beauveria bassiana expresses an endo-β-1,3-glucanase (BbEng1) functions helping cells recognition/ BbEng1 was specifically expressed infection, response cuticle hemolymph, presence osmotic or...
The dispensable maize (Zea mays L.) B chromosome is highly heterochromatic and widely believed to be devoid of functional genes. Although low-copy causes no obvious phenotype variation, its existence might influence A genome gene expression. Previous studies suggested that chromosomes are evolved from standard chromosomes; therefore, they contain genic regions showing homology with sequences. Our data influences the A-genome transcription stronger effect associated an increase in copy number...
WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins poplar defense are still largely unknown. In a previous study, we have shown that WRKY89 is induced by salicylic acid (SA) treatment and plays an role resistance against fungi transgenic poplars. Here, determined increase transcript levels Group IIa members poplars overexpressing using quantitative real-time polymerase chain reaction analysis. Yeast one-hybrid...
Although HsfA3 (heat-stress transcription factor A3) is well characterized in heat stress, its roles other abiotic stresses are less clear. In this study, we isolated two homologous genes, LlHsfA3A and LlHsfA3B, from lily (Lilium longiflorum). Both genes were induced by but not salt stress. Overexpressing Arabidopsis enhanced basal acquired thermotolerance, while overexpressing LlHsfA3B just thermotolerance. both cases, plants showed hypersensitivity to a lack of sucrose exacerbated...
Ionic aluminum (Al) in acidic soils, comprising approximately 50% of arable land globally, is highly toxic to most plant species. Populus grow naturally soils and tolerate high concentrations Al. Multidrug compound extrusion (MATE) family genes plants are involved responses Al tolerance. To date, however, the functional roles MATE remain unclear. In present study, 71 putative transporters were predicted genome trichocarpa. The chromosome distribution, phylogenetic relationships, expression...
It has been suggested that entomopathogenic fungi can be introduced into plants as endophytes potentially leading to insect control. Here, we sought identify specific strains of the pathogenic fungus, Beauveria bassiana would form endophytic associations with tobacco (Nicotiana benthamiana) benefitting host plant growth and/or resistance against pests and pathogens.Tobacco seeds were inoculated six different B. entophytic colonization, growth, pathogens evaluated over a 50 day-period....
Abstract The plant response to abiotic stresses involves both abscisic acid (ABA)-dependent and ABA-independent signaling pathways. Here we describe TaCHP, a CHP-rich (for cysteine, histidine, proline rich) zinc finger protein family gene extracted from bread wheat (Triticum aestivum), is differentially expressed during stress between the salinity-sensitive cultivar Jinan 177 its tolerant somatic hybrid introgression Shanrong No.3. TaCHP in roots of seedlings at three-leaf stage, transcript...
The underlying cause of spinal muscular atrophy (SMA) is a deficiency the survival motor neuron (SMN) protein. Starting from hits identified in high-throughput screening campaign and through structure-activity relationship investigations, we have developed small molecules that potently shift alternative splicing SMN2 exon 7, resulting increased production full-length SMN mRNA Three novel chemical series, represented by compounds 9, 14, 20, been optimized to increase level protein >50% SMA...
Abstract Secondary cell wall (SCW) biosynthesis during wood formation in trees is controlled by a multilevel regulatory network that coordinates the expression of substantial genes. However, few transcription factors involved negative regulation secondary have been characterized tree species. In this study, we isolated an R2R3 MYB factor MYB189 from Populus trichocarpa, which expressed predominantly vascular tissues, especially xylem. A novel repression motif was identified C-terminal region...
Laccases are widely present in bacteria, fungi, plants and invertebrates involved a variety of physiological functions. Here, we report that Beauveria bassiana, an economic important entomopathogenic fungus, secretes laccase 2 (BbLac2) during infection detoxifies insect immune response-generated reactive oxygen species (ROS) interferes with host phenoloxidase (PO) activation. BbLac2 is expressed fungal cells proliferation the haemocoel can be found to distribute on surface haemolymph-derived...
Summary MADS‐box transcription factor Mcm1 plays crucial roles in regulating mating processes and pathogenesis some fungi. However, its are varied fungal species, function remains unclear entomopathogenic Here, orthologue, Bbmcm1, was characterized a filamentous fungus, Beauveria bassiana . Disruption of Bbmcm1 resulted distinct reduction growth with abnormal conidiogenesis, significant decrease conidial viability germination. Δ displayed impaired cell integrity, distorted wall structure...
Drought is a major abiotic stress that limits plant growth and development. Adaptive mechanisms have evolved to mitigate drought stress, including the capacity adjust water loss rate modify morphology structure of epidermis. Here, we show expression CmNF-YB8, encoding nuclear factor Y (NF-Y) B-type subunit, lower under conditions in chrysanthemum (Chrysanthemum morifolium). Transgenic lines which transcript levels CmNF-YB8 were reduced by RNA interference (CmNF-YB8-RNAi) exhibited enhanced...
Background:Kandelia obovata, a dominant mangrove species, is widely distributed in tropical and subtropical areas. Low temperature the major abiotic stress that seriously limits survival growth of mangroves. WRKY transcription factors (TFs) play vital roles responses to biotic stresses. However, genome-wide analysis genes K. obovata their chilling have not been reported. Methods: Bioinformatic was used identify characterize (KoWRKY) gene family, RNA-seq qRT-PCR analyses were employed screen...
Jasmonic acid (JA) is a phytohormone involved in plant defense, growth, and development, etc. However, the regulatory mechanisms underlying JA-mediated lignin deposition secondary cell wall (SCW) formation remain poorly understood. In this study, we found that JA can inhibit SCW thickening poplar trees through exogenous MeJA treatment observation of phenotypes synthesis mutant, opdat1 . Hence, identified signal inhibitor PtoJAZ5, belonging to TIFY gene family, which regulation vascular...
The plant hormones jasmonic acid (JA) and salicylic (SA) play key roles in defenses against pathogens several WRKY transcription factors have been shown to a role SA/JA crosstalk. In previous study, overexpression of the poplar gene PtrWRKY89 enhanced resistance transgenic poplars. this promoter (ProPtrWRKY89) was isolated used drive GUS reporter gene. High activity observed old leaves Arabidopsis containing ProPtrWRKY89-GUS construct expression extremely induced by SA solution SA+MeJA...
Triacylglycerols (TAGs) are the major storage form of seed oil in oilseed plants. They biosynthesized de novo plastids and then transported into endoplasmic reticulum. However, transport mechanism for plastid fatty acids developing seeds remains unknown. Here, we isolated two novel acid exporters (FATTYACID EXPORT 2 [FAX2] FAX4, respectively) specifically abundant embryos during seed-filling stage Arabidopsis (Arabidopsis thaliana). FAX2 FAX4 were both localized to chloroplast membrane....