A5077072596- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Mycorrhizal Fungi and Plant Interactions
- Insect and Pesticide Research
- Plant Pathogens and Fungal Diseases
- Forest Insect Ecology and Management
- Studies on Chitinases and Chitosanases
- Insect Pest Control Strategies
- Insect Utilization and Effects
- Nematode management and characterization studies
- Fungal Biology and Applications
- Neurobiology and Insect Physiology Research
- Legume Nitrogen Fixing Symbiosis
- Enzyme Production and Characterization
- Forest Ecology and Biodiversity Studies
- Genomics and Phylogenetic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Invertebrate Immune Response Mechanisms
- Bacterial Genetics and Biotechnology
- Lichen and fungal ecology
- Insect-Plant Interactions and Control
University of Florida
2015-2025
University of Illinois Chicago
2023-2025
University of Illinois Urbana-Champaign
2024-2025
King's College London
2024-2025
University of Illinois System
2024
Chongqing University
2016-2023
Florida Museum of Natural History
2013-2022
Gainesville Obstetrics & Gynecology
2014
Johns Hopkins University
1988-2000
Pratt Institute
1996-2000
ABSTRACT The entomopathogenic fungus Beauveria bassiana produces at least three distinct single-cell propagules, aerial conidia, vegetative cells termed blastospores, and submerged which can be isolated from agar plates, rich broth liquid cultures, under nutrient limitation conditions in respectively. Fluorescently labeled fungal were used to quantify the kinetics of adhesion these cell types surfaces having various hydrophobic or hydrophilic properties. Aerial conidia adhered poorly weakly...
The entomopathogenic fungus Beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. Carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition immune invasion respect invertebrate hosts. B. produces number distinct cell types aerial conidia, submerged blastospores haemolymph-derived termed vivo or hyphal bodies. In order characterize variations surface...
Marked differences in surface characteristics were observed among three types of single-cell propagules produced by the entomopathogenic fungus Beauveria bassiana. Atomic force microscopy (AFM) revealed presence bundles or fascicles aerial conidia absent from vitro blastospores and submerged conidia. Contact angle measurements using polar apolar test liquids placed on cell layers used to calculate tension values free energies interaction with surfaces. These analyses indicated that surfaces...
Filamentous fungi are the most widely used eukaryotic biocatalysts in industrial and chemical applications. Consequently, there is tremendous interest methodology that can use power of genetics to develop strains with improved performance. For example, Metarhizium anisopliae a broad host range entomopathogenic fungus currently under intensive investigation as biologically based alternative pesticides. However, it limited by relatively low tolerance this species abiotic stresses such heat,...
Significance Although entomopathogenic fungi and their invertebrate hosts share a >300 million year co-evolutionary history, little is known concerning the biochemical genetic basis of insect defensive tactics countermeasures evolved evolving by pathogen to thwart these defenses. Our results provide molecular mechanism help explain why some insects are more resistant broad host-range fungi. We identify beetle cuticular secretions fungal detoxifying enzyme as components an arms race...
The regulatory network and biological functions of the fungal secondary metabolite oosporein have remained obscure. Beauveria bassiana has evolved ability to parasitize insects outcompete microbial challengers for assimilation host nutrients. A novel zinc finger transcription factor, BbSmr1 (B. regulator 1), was identified in a screen overproduction. Deletion Bbsmr1 resulted up-regulation biosynthetic gene cluster (OpS genes) constitutive production. Oosporein production abolished double...
Spodoptera frugiperda is a worldwide generalist pest with remarkable adaptations to environments and stresses, including developmental stage-related behavioral physiological adaptations, such as diverse feeding preferences, mate seeking, pesticide resistance. Insects' odorant-binding proteins (OBPs) chemosensory (CSPs) are essential for the chemical recognition during responses or other processes. The genome-wide identification gene expression patterns of all these identified OBPs CSPs...
Abstract Insects are one of the most successful animals in nature, and entomopathogenic fungi play a significant role natural epizootic control insect populations many ecosystems. The interaction between insects has continuously coevolved over hundreds millions years. Many components innate immune responses against fungal infection conserved across phyla. Additionally, behavioral responses, which include avoidance, grooming, and/or modulation body temperature, have been recognized as...
The Cordycipitaceae family of insecticidal fungi is widely distributed in nature, the most complex order Hypocreales (Ascomycota), with members displaying a diversity morphological characteristics and insect host ranges. Based on Bayesian evolutionary analysis five genomic loci(the small subunit ribosomal RNA (SSU) gene, large (LSU) translation elongation factor 1-α (tef1-α) largest polymerase II (rpb1), second (rpb2), we inferred divergence times for Cordycipitaceae, improving internal...
Nymphal and adult ticks from three different tick species, Dermacentor variabilis Say, Ixodes scapularis Rhipicephalus sanguineus Latrielle, were treated with conidia blastospores of the entomopathogenic fungi Beauveria bassiana (Bals.) Vuill. Metarhizium anisopliae Metschnikoff. Dose-response experiments indicated that a critical concentration fungal spores is required for infection mortality. Over 28-d time course, suspensions either B. or M. at 108 conidia/ml resulted in 50–70% mortality...
We have described some steps in chitin catabolism by Vibrio furnissii, and proposed that oligosaccharides are hydrolyzed the periplasmic space to GlcNAc (GlcNAc)2. Since (GlcNAc)2 is an important inducer cascade, it must resist hydrolysis periplasm.Known V. furnissii hydrolases comprise endoenzyme (Keyhani, N. O. Roseman, S. (1996) J. Biol. Chem. 271, 33414-33424), β-N-acetylglucosaminidase, ExoI, reported here. ExoI was isolated from a recombinant strain of Escherichia coli, hydrolyzes...
The entomopathogenic fungus Beauveria bassiana is under intensive study as a pest biological control agent. B. produces several distinct single-cell types that include aerial conidia, in vitro blastospores and submerged conidia. Under appropriate nutrient conditions these cells can elaborate germ tubes form hyphae, which turn lead to the formation of fungal mycelium. In addition, displays dimorphic transition, producing vivo specific yeast-like hyphal bodies during growth arthropod...