A5075124603- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Urological Disorders and Treatments
- Plant Pathogens and Fungal Diseases
- Plant Reproductive Biology
- Congenital Diaphragmatic Hernia Studies
- Urinary and Genital Oncology Studies
- Plant Physiology and Cultivation Studies
- Genomics and Phylogenetic Studies
- Soybean genetics and cultivation
- Plant Molecular Biology Research
- Plant and Fungal Interactions Research
- Nuts composition and effects
- Plant Gene Expression Analysis
- Sexual Differentiation and Disorders
- Horticultural and Viticultural Research
- Fatty Acid Research and Health
- Congenital Anomalies and Fetal Surgery
- Chromosomal and Genetic Variations
- Connective tissue disorders research
- Inflammasome and immune disorders
- Plant Virus Research Studies
- Cholesterol and Lipid Metabolism
- Ginseng Biological Effects and Applications
- Photosynthetic Processes and Mechanisms
Beijing Academy of Agricultural and Forestry Sciences
2008-2025
Shanghai Children's Hospital
2017-2024
Shanghai Jiao Tong University
2017-2024
China Agricultural University
2013-2021
Fudan University
2010-2017
Huadong Hospital
2010-2017
Sichuan University
2013
State Key Laboratory of Hydraulics and Mountain River Engineering
2013
Chinese Academy of Agricultural Sciences
2005
Producing maternal haploids via a male inducer can greatly accelerate maize (Zea mays) breeding process. However, the mechanism underlying haploid formation remains unclear. In this study, we constructed two lines containing cytogenetic marker B chromosome or alien centromeric histone H3 variant-yellow fluorescent protein vector to investigate mechanism. The as pollinators were crossed with hybrid ZhengDan958. chromosomes detected in F1 at low frequency, which was direct evidence support...
Peach (Prunus persica) is an economically important fruit crop and a well-characterized model for studying Prunus species. Here we explore the evolutionary history of peach using large-scale SNP data set generated from 58 high-coverage genomes cultivated closely related relatives, including 44 newly re-sequenced accessions 14 previous study. Our analyses suggest that originated about 2.47 Mya in southwest China glacial refugia by uplift Tibetan plateau. exploration genomic selection...
Abstract Background Structural variations (SVs), a major resource of genomic variation, can have profound consequences on phenotypic yet the impacts SVs remain largely unexplored in crops. Results Here, we generate high-quality de novo genome assembly for flat-fruit peach cultivar and produce comprehensive SV map peach, as high proportion sequence is occupied by heterozygous genome. We conduct population-level analyses that indicate undergone strong purifying selection during domestication,...
Pectobacterium spp. are necrotrophic bacterial plant pathogens of the family Pectobacteriaceae, responsible for a wide spectrum diseases important crops and ornamental plants including soft rot, blackleg, stem wilt. P. carotovorum is genetically heterogeneous species consisting three valid subspecies, subsp. brasiliense (Pcb), (Pcc), odoriferum (Pco). Thirty-two strains had their whole genomes sequenced, first complete genome Pco another circular Pcb, as well high-coverage sequences 30...
Abstract A narrow genetic basis in modern cultivars and strong linkage disequilibrium peach ( Prunus persica ) has restricted resolution power for association studies this model fruit species, thereby limiting our understanding of economically important quality traits including flavor. Here, we present a high-quality genome assembly Chinese landrace, Longhua Shui Mi (LHSM), representative the Cling peaches that have been central global improvement. We also map resequencing data 564...
Salicylic acid (SA) and reactive oxygen species (ROS) are two well-defined inducers of leaf senescence. Here, we identified a novel WRKY transcription factor gene WSR1 (WRKY regulating SA ROS 1) in Brassica napus (rapeseed) promoting production, which eventually led to senescence thereafter. Its expression increased senescing leaves. Ca2+-dependent protein kinase (CPK) 5 -6 interacted with phosphorylated BnaWSR1. Overexpression phosphomimic BnaWSR1 (BnaWSR1ca) rapeseed protoplasts elicited...
Rice bean (Vigna umbellata) is an underexploited domesticated legume crop consumed for dietary protein in Asia, yet little known about the genetic diversity of this species. Here, we present a high-quality reference genome rice landrace (FF25) built using PacBio long-read data and Hi-C chromatin interaction map, assess phylogenetic position speciation time within Vigna genus. We sequence 440 landraces (two core collections), GWAS based on growth sites at three widely divergent latitudes...
Background . The aim of this study is to investigate whether (-)-epigallocatechin-3-gallate (EGCG) can prevent the UA-induced inflammatory effect human umbilical vein endothelial cells (HUVEC) and involved mechanisms in vitro. Methods HUVEC were subjected uric acid (UA) with or without EGCG treatment. RT-PCR western blots performed determine level inflammation marker. antioxidant activity was evaluated by measuring scavenged reactive oxygen species (ROS). Functional studies role Notch-1...
Expressed sequence tag (EST) derived simple repeats (SSRs, microsatellites) were screened and identified from 3863 almond 10 185 peach EST sequences, the spectra of SSRs in non-redundant sequences investigated after assembly. One hundred seventy-eight (12.07%) 497 (9.97%) detected. The EST-SSR occurs every 4.97 kb ESTs 6.57 peach, with di- trinucleotide repeat motifs are most abundant both ESTs. Twenty one EST-SSRs thereafter, developed used together 7 genomic SSRs, to study genetic...
Chinese cabbage (Brassica rapa subsp. pekinensis) suffers from soft rot disease caused by Pectobacterium carotovorum (Pc). To uncover the mechanisms underlying defense response of to Pc, we constructed a suppression subtractive hybridization (SSH) library Pc-infected and obtained 1 919 non-redundant expressed sequence tags (ESTs), which were used for cDNA microarray. We detected 800 differentially genes (DEGs) in at different time points post-Pc inoculation, further confirmed quantitative...
Pectobacterium carotovorum, a causal agent of vegetable soft rot, contains three valid subspecies: P. carotovorum subsp. (Pcc), brasiliensis (Pcb), and odoriferum (Pco). Using 16S rDNA sequencing genus-specific PCR, we identified 72 strains from Chinese cabbage, bok choy, celery assessed their pathogenicity on cabbage petioles potato tubers. Based phylogenetic analysis pmrA sequences confirmation by subspecies-specific the were divided into 18 Pcc, 29 Pco, 25 Pcb. Several characteristic...
Abstract Background The aberrant expression of genes involved in androgen metabolism and genetic contribution are unclear hypospadias. Methods We compared gene profiles by RNA sequencing from five non‐hypospadiac foreskins, mild hypospadiac severe foreskins. In addition, to identify rare coding variants with large effects on hypospadias risk, we carried out whole exome three patients a family. Results average receptor ( AR ) CYP19A1 were significantly decreased p < .01) .05), whereas...
Bacterial soft rot is an important disease of Chinese cabbage (Brassica rapa L. ssp. pekinensis) in China and many other countries. Four pectinolytic bacterial strains (WBC1, WBC6, WBC9, WBC11) were isolated from soft-rotted Beijing, China. Based on 16S rDNA pmrA gene sequence analyses, multilocus analysis (MLSA), genomic average nucleotide identity (ANI) analysis, these four identified as Pectobacterium polaris. This species, previously reported potato countries not including China, a new...
Modulation of n-3 fatty acids on genetic susceptibility to type 2 diabetes (T2D) is still not clear. In a case-control study 622 Chinese T2D patients and 293 healthy controls, risk score (GRS) was created based nine variants. Logistic regression used examine the interaction GRS with erythrocyte phospholipid for risk. Every 1-unit (corresponding 1 allele) increase in associated 12% (Odds ratio (OR): 1.12; 95% confidence intervals (CI): 1.04–1.20) higher T2D. Compared lowest quartile,...
Abstract Objective: To investigate the roles of transcription factors (TFs) in etiology complex human birth defects, including neural tube defects (NTDs), congenital heart diseases (CHDs), and hypospadias. Methods: We examined overlap genetically associated genes among NTDs, CHDs, then compared expression profiles these based on all detected disease-associated TFs. The differentially expressed TFs that we obtained were further subjected to functional enrichment analysis elucidate their role...
Previous genome-wide association studies have identified variants in the diacylglycerol kinase kappa (DGKK) gene associated with hypospadias populations of European descent. However, no DGKK were confirmed to be a recent Han Chinese study population, likely due limited number single-nucleotide polymorphisms (SNPs) included analysis. In this study, we aimed address inconsistent results and evaluate between population through more comprehensive analysis variants. We conducted analyses for 17...
Abstract Background Hypospadias risk–associated gene variants have been reported in populations of European descent using genome-wide association studies (GWASs). There is little known at present about any possible hypospadias risk associations Han Chinese populations. Methods To systematically investigate patients, we performed the first GWAS a cohort consisting 197 moderate-severe cases and 933 unaffected controls. Suggestive loci ( p < 1 × 10 − 4 ) were replicated 118 383 controls, as...