A5029001134- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Autophagy in Disease and Therapy
- Fungal Biology and Applications
- Plant Disease Resistance and Genetics
- Cellular transport and secretion
- Plant Parasitism and Resistance
- Ferroptosis and cancer prognosis
- Mycorrhizal Fungi and Plant Interactions
- Genetic Neurodegenerative Diseases
- Microtubule and mitosis dynamics
- Microbial Natural Products and Biosynthesis
- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- Antifungal resistance and susceptibility
- Plant Virus Research Studies
- Studies on Chitinases and Chitosanases
- Tannin, Tannase and Anticancer Activities
- Cardiomyopathy and Myosin Studies
- Insect-Plant Interactions and Control
- Plant responses to water stress
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- GABA and Rice Research
National University of Singapore
2015-2024
Temasek Life Sciences Laboratory
2015-2024
Nanyang Technological University
2010-2018
Washtenaw Community College
2012
University of Michigan
2012
UiT The Arctic University of Norway
2012
Institute of Molecular and Cell Biology
2001
Howard Hughes Medical Institute
1998
Vanderbilt University
1998
Maharaja Sayajirao University of Baroda
1995-1996
Changes in global temperatures profoundly affect the occurrence of plant diseases. It is well known that rice blast can easily become epidemic relatively warm weather. However, molecular mechanism remains unclear. In this study, we show enhanced development at a temperature (22°C) compared with normal growth (28°C) plant-determined. Comparative transcriptome analysis revealed jasmonic acid (JA) biosynthesis and signaling genes could be effectively induced by Magnaporthe oryzae 28°C but not...
Abstract Schizosaccharomyces pombe is an excellent organism in which to study cytokinesis as it divides by medial fission using F-actin contractile ring. To enhance our understanding of the cell division process, a large genetic screen was carried out 17 loci essential for were identified, 5 are novel. Mutants identifying three genes, rng3+, rng4+, and rng5+, defective organizing actin Four mutants septum deposition, initiation (sid)1, sid2, sid3, sid4, also identified characterized. Genetic...
Summary We identified the M agnaporthe oryzae avirulence effector A vr P i9 cognate to rice blast resistance gene by comparative genomics of requisite strains derived from a sequential planting method. AvrPi9 encodes small secreted protein that appears localize in biotrophic interfacial complex and is translocated host cell during infection. forms tandem array with its paralogue proximal centromeric region chromosome 7. expressed highly at early stages initiation disease. Virulent isolate...
The interaction of Magnaporthe oryzae, the rice blast fungus, and begins when M. oryzae establishes contact with host plant surface. On perception appropriate surface signals, forms appressoria initiates invasion. Pth11, an important G-protein-coupled receptor necessary for appressorium formation in contains seven transmembrane regions a CFEM (common several fungal extracellular membrane proteins) domain characteristic eight cysteine residues. We focused on gaining further insight into role...
Summary Ferroptosis, an iron‐dependent cell death process, was found to occur in Magnaporthe oryzae , and plays a key role infection‐related development therein. Ferroptosis the rice‐blast fungus confirmed based on five basic criteria. We dependence of ferroptosis ferric ions, optimized ratio‐fluorescence imaging C11‐BODIPY 581/591 as precise sensor for lipid peroxides that mediate M. . uncovered important regulatory function reduced glutathione NADPH oxidases modulating superoxide moieties...
The Woronin body is a peroxisome-derived dense-core vesicle that specific to several genera of filamentous ascomycetes, where it has been shown seal septal pores in response cellular damage. Hexagonal peroxisome (Hex1) protein was recently identified as major constituent the and be responsible for self-assembly dense core this organelle. Using mutation Magnaporthe grisea HEX1 ortholog, we define dual essential function bodies during pathogenic phase rice blast fungus. We show initially...
Summary In lower eukaryotes, beta‐oxidation of fatty acids is restricted primarily to the peroxisomes and resultant acetyl‐CoA molecules (and chain‐shortened acids) are transported via cytosol into mitochondria for further breakdown usage. Using a loss‐of‐function mutation in Magnaporthe grisea PEROXIN6 orthologue, we define an essential role peroxisomal during host invasion step rice‐blast disease. We show that Mgpex6Δ strain lacks functional incapable β‐oxidation long‐chain acids. The...
AbstractAutophagy, a conserved pathway for bulk cellular degradation and recycling in eukaryotes, regulates proper turnover of organelles, membranes certain proteins. Such regulated is important cell growth development particularly during environmental stress conditions, which act as key inducers autophagy. We found that autophagy MoATG8 were significantly induced asexual Magnaporthe oryzae. An RFP-tagged MoAtg8 showed specific localization enrichment aerial hyphae, conidiophores conidia....
Fatty acids stored as triglycerides, an important source of cellular energy, are catabolized through β-oxidation pathways predicted to occur both in peroxisomes and mitochondria filamentous fungi. Here, we characterize the function Enoyl-CoA hydratase Ech1, a mitochondrial enzyme, model phytopathogen Magnaporthe oryzae. Ech1 was found be essential for conidial germination viability older hyphae. Unlike wild-type Magnaporthe, ech1Δ failed utilize C14 fatty acid partially impeded growth on C16...
The retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, role of such events during pathogen-host interaction remains unclear. Here, we report that cargo-recognition complex (MoVps35, MoVps26 and MoVps29) is essential for appressorium-mediated host penetration by Magnaporthe oryzae, causal pathogen blast disease rice. Loss function blocked glycogen distribution turnover lipid bodies, delayed nuclear degeneration...
Magnaporthe oryzae, the ascomycete fungus that causes rice blast disease, initiates conidiation in response to light when grown on Prune-Agar medium containing both carbon and nitrogen sources. Macroautophagy/autophagy was shown be essential for M. oryzae induced specifically upon exposure but is undetectable dark. Therefore, it inferred autophagy naturally by light, rather than starvation during conidiation. However, signaling pathway(s) involved such phototropic induction of remains...
Summary Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host downregulate immunity and promote infection. Here, we uncover fascinating membrane trafficking delivery route originates from vacuolar membranes in Magnaporthe oryzae conduits interface plasma membrane. To perform such secretory/trafficking function, MoRab7 first recruits retromer complex membrane, enabling recognition of family SNARE proteins, including MoSnc1. Live‐cell imaging...
Abstract In prokaryotes and eukaryotes, multidrug resistance (MDR) transporters use energy-dependent efflux action to regulate the intracellular levels of antibiotic or xenobiotic compounds. Using mutational analysis ABC3, we define an important role for such MDR-based during host penetration step Magnaporthe grisea pathogenesis. Mutants lacking ABC3 were completely nonpathogenic but surprisingly capable penetrating thin cellophane membranes some extent. The inability abc3Δ penetrate surface...
Cyclic AMP-dependent pathways mediate the communication between external stimuli and intracellular signaling machinery, thereby influencing important aspects of cellular growth, morphogenesis differentiation. Crucial to proper function robustness these cascades is strict regulation maintenance levels cAMP through a fine balance biosynthesis (by adenylate cyclases) hydrolysis phosphodiesterases). We functionally characterized gene-deletion mutants high-affinity (PdeH) low-affinity (PdeL)...
In Magnaporthe oryzae, the causal ascomycete of devastating rice blast disease, conidial germ tube tip must sense and respond to a wide array requisite cues from host in order switch polarized isotropic growth, ultimately forming dome-shaped infection cell known as appressorium. Although role for G-protein mediated Cyclic AMP signaling appressorium formation was first identified almost two decades ago, little is about spatio-temporal dynamics cascade how signal transmitted through...
Macroautophagy-mediated glycogen catabolism is required for asexual differentiation in the blast fungus, Magnaporthe oryzae. However, function(s) of selective subtypes autophagy has not been studied therein. Here, we report that mitophagy, autophagic delivery mitochondria to vacuoles degradation, occurs during early stages conidiation. Specifically, mitophagy was evident foot cells while being undetectable aerial hyphae and/or conidiophores. We show loss MoAtg24, a sorting nexin related...
Flavonoids are polyphenolic secondary metabolites that function as signaling molecules, allopathic compounds, phytoalexins, detoxifying agents and antimicrobial defensive compounds in plants. Blast caused by the fungus Magnaporthe oryzae is a serious disease affecting rice cultivation. In this study, we revealed natural flavonoid, tangeretin, substantially delays formation of M. appressoria blocks development blast lesions on Our data suggest tangeretin has antioxidant activity interferes...