A5029573493- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Insect symbiosis and bacterial influences
- Biofuel production and bioconversion
- Polyamine Metabolism and Applications
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Fungal Biology and Applications
- Fermentation and Sensory Analysis
- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- Microbial Metabolism and Applications
- Polysaccharides and Plant Cell Walls
- Plant Pathogenic Bacteria Studies
- Tannin, Tannase and Anticancer Activities
- Ferroptosis and cancer prognosis
- Sugarcane Cultivation and Processing
- Autophagy in Disease and Therapy
South China Agricultural University
2019-2024
Key Laboratory of Guangdong Province
2022-2023
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2019-2023
Shaoguan University
2023
Flavonoids are polyphenolic secondary metabolites that function as signaling molecules, allopathic compounds, phytoalexins, detoxifying agents and antimicrobial defensive compounds in plants. Blast caused by the fungus Magnaporthe oryzae is a serious disease affecting rice cultivation. In this study, we revealed natural flavonoid, tangeretin, substantially delays formation of M. appressoria blocks development blast lesions on Our data suggest tangeretin has antioxidant activity interferes...
The rice blast fungus Magnaporthe oryzae has been known to produce the phytohormone auxin/IAA from its hyphae and conidia, but detailed biological function biosynthesis pathway is largely unknown. By sequence homology, we identified a complete indole-3-pyruvic acid (IPA)-based IAA in M. oryzae, consisting of tryptophan aminotransferase (MoTam1) indole-3-pyruvate decarboxylase (MoIpd1). In comparison wild type, production was significantly reduced motam1Δ mutant, further moipd1Δ mutant....
In this study, we isolated an endophytic Burkholderia gladioli strain, named CGB10, from sugarcane leaves. B. CGB10 displayed strong inhibitory activity against filamentous growth of fungal pathogens, one which is Sporisorium scitamineum that causes smut, a major disease affecting the quality and production in tropical subtropical regions. could effectively suppress smut under field conditions, without itself causing any obvious damage or disease, thus underscoring great potential as...
The sugarcane smut fungus Sporisorium scitamineum is bipolar and produces sporidia of two different mating types. During infection, haploid cells opposite types can fuse to form dikaryotic hyphae that colonize plant tissue. Mating filamentation are therefore essential for S. pathogenesis. In this study, we obtained one T-DNA insertion mutant disrupted in the gene encoding pheromone response factor (Prf1), hereinafter named SsPRF1, scitamineum, via Agrobacterium tumefaciens-mediated...
Sugarcane smut caused by the basidiomycete fungus S. scitamineum leads to massive economic losses in sugarcane plantation globally. Dikaryotic hyphae formation (filamentous growth) and biofilm are two important aspects pathogenesis, yet molecular regulation of these processes was not as extensively investigated that model pathogenic fungi, e.g., Candida albicans , Ustilago maydis or Cryptococcus neoformans .
Sporisorium scitamineum is the fungal pathogen causing severe sugarcane smut disease that leads to massive economic losses globally. S. invades host cane by dikaryotic hyphae, formed after sexual mating of two haploid sporidia opposite type. Therefore, mating/filamentation critical for pathogenicity, while its molecular mechanisms remain largely unknown. The AGC (cyclic AMP [cAMP]-dependent protein kinase 1 [protein A {PKA}], cGMP-dependent [PKG], and C [PKC]) family a group serine/threonine...
Abstract Sugarcane smut fungus Sporisorium scitamineum produces polyamines putrescine (PUT), spermidine (SPD), and spermine (SPM) to regulate sexual mating/filamentous growth critical for pathogenicity. Besides de novo biosynthesis, intracellular levels of could also be modulated by oxidation. In this study, we identified two annotated polyamine oxidation enzymes (SsPAO SsCuAO1) in S. . Compared the wild type ( MAT‐1 ), ss1pao Δ ss1cuao1 mutants were defective sporidia growth,...
The ascomycete fungus Magnapothe oryzae is the causal agent of rice blast disease, leading to severe loss in cultivated production worldwide. In this study, we identified a conserved type 2 glycosyltransferase named MoGt2 M. . mogt2 Δ targeted gene deletion mutants exhibited pleiotropic defects vegetative growth, conidiation, stress response, hyphal appressorium-mediated penetration, and pathogenicity. Furthermore, domains are critical for function. comparative transcriptome analysis...
The basidiomycetous fungus Sporisorium scitamineum causes sugarcane smut that leads to severe economic losses in the major growing areas China, India and Brazil, etc. Autophagy is a conserved pathway eukaryotes for bulk degradation cellular recycling, was shown be important fungal cell growth, development, pathogenicity. However, physiological function of autophagy has not been studied S. scitamineum. In this study, we identified Atg8 protein, named as SsAtg8 characterized its function. Our...
Sporisorium scitamineum, the basidiomycetous fungus causing sugarcane smut that leads to severe losses in quantity and quality, undergoes sexual mating form dikaryotic hyphae capable of invading host cane. Therefore, suppressing formation would potentially be an effective way prevent infection by fungus, following disease symptom development. The phytohormone methyl jasmonate (MeJA) has been showed induce plant defense against insects microbial pathogens. In this study, we verified exogenous...
Sporisorium scitamineum, the basidiomycetous fungus that causes sugarcane smut and leads to severe losses in quantity quality, undergoes sexual mating form dikaryotic hyphae capable of invading host cane. Therefore, suppressing formation would potentially be an effective way prevent infection by fungus, following disease symptom developments. The phytohormone methyl jasmonate (MeJA) has been shown induce plant defenses against insects microbial pathogens. In this study, we will verify...
Abstract Sugarcane smut caused by Sporisorium scitamineum seriously impairs sugarcane production and quality. Sexual mating/filamentation is a critical step of S. pathogenesis, yet the regulatory mechanisms are not fully understood. In this study, we identified SsAGA , SsODC SsSAMDC genes, which involved in polyamine biosynthesis . Deletion led to complete loss filamentous growth after sexual mating, deletion or reduced filamentation. Double resulted auxotrophy for putrescine (PUT)...