Yaws, caused by Treponema pallidum ssp. pertenue, is reportedly endemic in Ghana. Mass distribution of azithromycin now the cornerstone WHO yaws eradication campaign. at a lower target dose was previously undertaken two regions Ghana for control trachoma. Ongoing reporting raises possibility that resistance may have emerged T. or alternative infections be responsible some reported cases. We conducted cross-sectional survey thirty communities districts where MDA trachoma had been conducted....

Summary The ferric iron‐binding protein (Fbp) expressed by pathogenic Neisseria spp. has been proposed to play a central role in the high‐affinity acquisition of iron from human transferrin. results this investigation provide evidence that Fbp participates process as functional analogue Gram‐negative periplasmic‐binding component, which operates part general active transport for receptor‐mediated, Known properties are correlated with those other well‐characterized proteins, including...

ABSTRACT Macrolide treatment failure in syphilis patients is associated with a single point mutation (either A2058G or A2059G) both copies of the 23S rRNA gene Treponema pallidum strains. The conventional method for detection mutations uses nested PCR combined restriction enzyme digestions, which laborious and time-consuming. We initially developed TaqMan-based real-time duplex assay mutation, upon discovery A2059G we modified into triplex format to simultaneously detect mutations. detected...

The rice blast fungus Magnaporthe oryzae has been known to produce the phytohormone auxin/IAA from its hyphae and conidia, but detailed biological function biosynthesis pathway is largely unknown. By sequence homology, we identified a complete indole-3-pyruvic acid (IPA)-based IAA in M. oryzae, consisting of tryptophan aminotransferase (MoTam1) indole-3-pyruvate decarboxylase (MoIpd1). In comparison wild type, production was significantly reduced motam1Δ mutant, further moipd1Δ mutant....

A multiplex polymerase chain reaction (M-PCR) assay that simultaneously detects the three major causes of genital ulcer disease (GUD), Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus, was used to evaluate swab specimens for 38 sequential patients with GUD at a Thai sexually transmitted clinic. Subjects received clinical diagnoses syndromic treatment. Swab H. ducreyi cultures M-PCR were obtained. No positive. Of specimens, 31 (81.6%) positive HSV, 1 (2.3%) both HSV T. none...

ABSTRACT We report on a case of gastric syphilis in patient with chronic dyspepsia. The diagnosis was established by serology and the demonstration spirochetes diffusely inflammed mucosa staining fluorescent monoclonal antibody specific for pathogenic treponemes detection treponemal DNA sequences real-time PCR.

In Brief Objectives: The objectives of this study were to evaluate the use a real-time multiplex polymerase chain reaction (M-PCR) assay differentiate between trachoma and lymphogranuloma venereum (LGV) biovars Chlamydia trachomatis validate its performance with conventional genotyping method. Study: Swab specimens from 115 patients anorectal symptoms or syndromes associated LGV tested by M-PCR results compared PCR-based restriction fragment length polymorphism analysis major outer membrane...

The experimental demonstrations of light-emitting diode (LED) fabrication with surface plasmon (SP) coupling the radiating dipoles in its quantum wells are first reviewed. SP a dipole can create an alternative emission channel through radiation for enhancing effective internal efficiency when intrinsic non-radiative recombination rate is high, reducing external droop effect at high current injection levels, and producing partially polarized LED output by inducing polarization-sensitive...

Ulcer material from consecutive patients attending clinics in Antananarivo, Madagascar, was tested using multiplex polymerase chain reaction (M-PCR) to detect Treponema pallidum, Haemophilus ducreyi, and herpes simplex virus. Sera were for syphilis IgG IgM antibodies Chlamydia trachomatis by microimmunofluorescence testing (MIF). By M-PCR, 33% of 196 had chancroid, 29% syphilitic ulcers, 10% genital herpes; 32% the ulcer specimens M-PCR negative. Compared with serology 72% sensitive 83%...

Soil-borne beneficial microbes establish symbioses with plant hosts and play key roles during growth development therein. In this study, two fungal strains, FLP7 B9, were isolated from the rhizosphere microbiome associated Choy Sum (Brassica rapa var. parachinensis) barley (Hordeum vulgare), respectively. Sequence analyses of internal transcribed spacer 18S ribosomal RNA genes combined colony conidial morphology identified B9 to be Penicillium citrinum strains/isolates. Plant–fungus...

The iron-storage protein bacterioferritin (Bfr) from Neisseria gonorrhoeae strain F62 was identified in cell-free extracts and subsequently purified by column chromatography. Gonococcal Bfr had an estimated molecular mass of 400 kDa gel filtration; however, analysis SDS-PAGE revealed that it composed 18 (BfrA) 22 (BfrB) subunits. DNA encoding BfrB amplified PCR using degenerate primers derived the N-terminal amino acid sequence a C-terminal Escherichia coli Bfr. bfrA obtained genome walking...

Previous work in 3 subjects infected for 2 weeks indicated that experimental infection with Haemophilus ducreyi recruits CD4 cells to the skin at pustular stage of disease. In order describe kinetics host response, 23 were sites a standardized dose H ducreyi. Subjects biopsied 1 or 4 days after inoculation when they developed painful lesion (days 7–14). Papules and pustules contained predominant T cell infiltrate consisted CD45RO αβ lineage. Both papules mixed helper type cytokine mRNA...

In Brief Objective: The objective of this study was to determine predictors Trichomonas vaginalis among women and their partners in Moshi, Tanzania. Study Design: Women (N = 1440) 588) were interviewed specimens for detection T. sexually transmitted infections (STIs) collected. Results: Prevalence 10.7% 6.3% men. Having a partner with the strongest risk factor (adjusted odds ratio [OR], 19.44; 95% confidence interval [CI], 7.84–48.25) men OR, 19.01; CI, 6.8–52.40). Risk infection increased...

In this paper, the feasibility and effectiveness of using an air-plasma torch to clot blood are studied. The emission spectroscopy temperature measurement show that produces abundant atomic oxygen in plasma effluent. Anticoagulated whole-blood samples (10 mulscr ) were tested for two cases: 1) sample exposed effluent 2) a heated airflow; both cases, increase was controlled be about same. No indication coagulation observed second case; on other hand, first case when directly effluent, shell...

Abstract The female genital tract (FGT) provides a means of entry to pathogens, including HIV, yet immune cell populations at this barrier between host and environment are not well defined. We initiated study healthy women characterize resident T in the lower FGT from lavage patient-matched peripheral blood investigate potential mechanisms HIV sexual transmission. Surprisingly, we observed CD4 were primarily CCR7hi, consistent with central memory or recirculating phenotype. In addition,...

Bisdemethoxycurcumin (BDMC) exhibits biological activities including anticancer and anti-metastasis in human cancer cell lines, but there is no available information to show whether BDMC suppresses migration invasion of cervical cells.Wound-healing, migration, invasion, zymography, western blotting assays were used investigate the effects on HeLa cells vitro.BDMC reduced total viable number a dose-dependent manner. The wound-healing assay suppressed movement cells. Furthermore, trans-well...

We measured the temperature-dependent photoluminescence (PL) decay time of mixed system free exciton (FX) and donor-bound (DX0) in a ZnO thin film. From cw PL spectra, we also calibrated individual spectral widths integrated intensities FX DX0 by fitting spectra with four Lorentzian functions. The radiative lifetime was then based on thermal quenching rate total intensity system. With data, lifetimes were estimated using theoretical relation between width. results lifetime, considering...

The authors analyze the contribution of screening quantum-confined Stark effect (QCSE) to emission enhancement behavior in process surface plasmon (SP) coupling with an InGaN∕GaN quantum well (QW), which is 20nm away from a Ag thin film that supports SP. From measurements excitation power dependent photoluminescence and time-resolved (TRPL) spectroscopy, fitting TRPL data based on rate-equation model, it found when level high, QCSE not only contributes significantly but also increases SP...

A portable low temperature air plasma torch was used to control bleeding from wounds. As animal models, two pigs were in the tests: one for treatment, and other as untreated control. Plasma effects on times of three types wounds (straight cut cross ham area, a hole saphenous vein an ear) examined. The results that this shortened time these about 3 min 18 s, 4 13 88 s 15 respectively. Emission spectroscopy performed explore reactive species carried by effluent torch. show carries abundant...

ABSTRACT We used PCR assays to determine the etiology of genital ulcers in patients presenting a sexually transmitted disease clinic Dakar, Senegal, and evaluated ability two tests ( groEL recD ) serological (adsorption enzyme immunoassay [EIA] lipooligosaccharide [LOS] EIA) detect current Haemophilus ducreyi infection. found that this population, H. , T. pallidum herpes simplex virus HSV DNA were detected 56, 15, 13% 39 ulcer specimens, respectively, was 60% (3 5) samples from ulcerated...

ABSTRACT We report a case of yaws in patient with puritic cutaneous eruption who was initially suspected infection monkeypox. The diagnosis established by real-time PCR and sequencing specific treponemal DNA sequences. This is the first describing use to identify Treponema pallidum subsp. pertenue -specific sequences active yaws.