A5070735649- Genetics, Aging, and Longevity in Model Organisms
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Wnt/β-catenin signaling in development and cancer
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Biocrusts and Microbial Ecology
- Advanced Vision and Imaging
- Connective tissue disorders research
- Bacterial Genetics and Biotechnology
- Congenital heart defects research
- Vibrio bacteria research studies
- Epigenetics and DNA Methylation
- Genetic Neurodegenerative Diseases
- Signaling Pathways in Disease
- Circadian rhythm and melatonin
- RNA and protein synthesis mechanisms
- Cell death mechanisms and regulation
- Cardiomyopathy and Myosin Studies
- Ubiquitin and proteasome pathways
- Advanced Image Processing Techniques
- Listeria monocytogenes in Food Safety
Ashoka University
2011-2024
National University of Singapore
2002-2019
Temasek Life Sciences Laboratory
2010-2012
Bioprocessing Technology Institute
2007
National Institutes of Health
1993
National Cancer Institute
1993
Epithelial (E)-cadherin-mediated cell-cell junctions play important roles in the development and maintenance of tissue structure multicellular organisms. E-cadherin adhesion is thus a key element cellular microenvironment that provides both mechanical biochemical signaling inputs. Here, we report vitro reconstitution junction-like structures between native living cells extracellular domain (E-cad-ECD) supported membrane. Junction formation this hybrid live cell-supported membrane...
In many eukaryotes, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring. Despite central role this ring in cytokinesis, mechanism F-actin accumulation is not fully understood. paper, we investigate during Schizosaccharomyces pombe using lifeact as a probe to monitor actin dynamics. Previous work has shown that actomyosin assembled de novo at division site. Surprisingly, find significant fraction was recruited from formin-Cdc12p nucleated long cables...
Significance Salmonella resides as an intracellular pathogen in eukaryotic hosts and causes diarrhea or typhoid fever. In a small but alarming proportion of infected humans, exists asymptomatically biofilms, basis for long-term disease transmission. Although biofilms have been observed chronically mice, mechanisms underlying why how such multicellular aggregates are formed vivo remain poorly understood. We exposed optically transparent Caenorhabditis elegans to the progression infection...
Syncytial architecture is an evolutionarily-conserved feature of the germline many species and plays a crucial role in their fertility. However, mechanism supporting syncytial organization largely unknown. Here, we identify corset-like actomyosin structure within Caenorhabditis elegans, surrounding common rachis. Using laser microsurgery, demonstrate that contractility this generates tension both plane rachis surface perpendicular to it, opposing membrane tension. Genetic pharmacological...
A novel reverse genetic approach termed 'marker reconstitution mutagenesis' was designed to generate mutational allelic series in genes of interest. This consists two simple steps which utilize selective markers. First, using one marker, a partial fragment another marker gene is inserted adjacently interest by homologous recombination. Second, random mutations are introduced precisely into the interest, together with latter successfully tested for several fission yeast Schizosaccharomyces...
Cell fate determination in the early mammalian embryo is regulated by multiple mechanisms. Recently, genes involved vesicular trafficking have been shown to play an important role cell choice, although regulation of their expression remains poorly understood. Here we demonstrate for first time that endocytosis associated (EAGs) are repressed through a novel, dual mechanism mouse embryonic stem cells (mESCs). This involves action Polycomb Repressive Complex, PRC2, as well post-transcriptional...
Biochemical signaling arising from mechanical force-induced physical changes in biological macromolecules is a critical determinant of key physiological processes across all lengths and time scales. Recent studies have deepened our understanding how mechano-transduction regulates somatic tissues such as those alveolar, gastrointestinal, embryonic, skeleto-muscular systems. The germline an organism has heterogeneous composition - germ cells at different stages maturation mature gametes, often...
The regulatory and catalytic subunits of cAMP-dependent protein kinase (PKA) were coexpressed within the same bacterial cell using a polycistronic T7 expression vector encoding Chinese hamster cDNAs for type I (RI) alpha (C alpha) PKA. Basal active RI/C holoenzyme in BL21(DE3) strain Escherichia coli caused severe growth inhibition resulting extremely small colony size. Several lines evidence demonstrate that this requires PKA cAMP: (i) phenotype is dependent on cAMP since it not seen...
SUMMARY In metazoan cells, the delicate equilibrium between proliferative cell division and inter-cellular adhesion is crucial for achieving growth while maintaining tissue integrity homeostasis. The transmembrane receptor, E-cadherin, not only facilitates cell-cell but modulates cytokinesis by impeding cortical F-actin flow inhibiting actomyosin contractility. C. elegans zygote, newly established interfaces following lack E-cadherin/HMR-1 (Padmanabhan et al., 2017b). mechanisms responsible...