Fatty acids stored as triglycerides, an important source of cellular energy, are catabolized through β-oxidation pathways predicted to occur both in peroxisomes and mitochondria filamentous fungi. Here, we characterize the function Enoyl-CoA hydratase Ech1, a mitochondrial enzyme, model phytopathogen Magnaporthe oryzae. Ech1 was found be essential for conidial germination viability older hyphae. Unlike wild-type Magnaporthe, ech1Δ failed utilize C14 fatty acid partially impeded growth on C16...

Polarized growth is essential for cellular development and function requires coordinated organization of the cytoskeletal elements. Tea4, an important polarity determinant, regulates localized F-actin assembly bipolar in fission yeast directional mycelial Aspergillus. Here, we characterize Tea4 rice blast fungus Magnaporthe oryzae (MoTea4). Similar to its orthologs, MoTea4-green fluorescent protein (MoTea4-GFP) showed punctate distribution confined zones, particularly tips, aerial hyphae,...

Magnaporthe oryzae, which causes the devastating rice-blast disease, invades its host plants via a specialized infection structure called appressorium. Previously, we showed that ATP-Binding Cassette 3 transporter is necessary for appressorial function (host penetration) in M. oryzae. However, thus far, molecular basis underlying impaired abc3Δ remains elusive. We hypothesized appressoria accumulate excessive amounts of specific efflux substrate(s) Abc3 devised an innovative yeast-based...

Two-component signal transduction (TCST) pathways play crucial roles in many cellular functions such as stress responses, biofilm formation and sporulation. The histidine phosphotransferase (HPt), which is an intermediate phosphotransfer protein a two-component system, transfers phosphate group to phosphorylatable aspartate residue the target protein(s), up-regulates stress-activated MAP kinase cascades. Most fungal genomes carry single copy of gene coding for HPt, are potential antifungal...

A new gene-tagging method (marker fusion tagging [MFT]) is demonstrated for Neurospora crassa and Magnaporthe oryzae. Translational fusions between the hygromycin B resistance gene various markers are inserted into genes of interest by homologous recombination to produce chromosomally encoded proteins. This can tags at any position create deletion alleles that maintain N- C-terminal sequences. We show utility MFT producing enhanced green fluorescent protein (EGFP) in proteins localized...

The outer kinetochore DASH complex (also known as the Dam1 complex) ensures proper spindle structure and chromosome segregation. While protein requirement diverges among different yeasts, its role in filamentous fungi has not yet been investigated. We studied dynamics of middle (Mis12) (Dam1 Ask1) proteins fungal pathogen, Magnaporthe oryzae, which undergoes multiple cell cycle-linked developmental transitions. Mis12 was constitutively present nucleus, Ask1 were recruited only during...

A key question that has remained unanswered is how pathogenic fungi switch from vegetative growth to infection-related morphogenesis during a disease cycle. Here, we identify fungal oxylipin analogous the phytohormone jasmonic acid (JA), as principal regulator of such developmental isotropic and pathogenicity in rice-blast fungus Magnaporthe oryzae. Using specific inhibitors mutant analyses, determined molecular function intrinsic jasmonates M. oryzae pathogenesis. Loss 12-Oxo-phytodienoic...

During mixed-species invasive candidiasis - an emerging threat in healthcare presence of Candida albicans is near-essential for host colonisation by C. glabrata . However, the molecular communication underlying such intriguing inter-species interaction largely unexplored. Here, we show that secretes a unique small protein Yhi1 induces hyphal growth , which essential tissue invasion. Notably, this Yhi1-based specific to and when compared with other common species Our structure-function...

Abstract A key question that has remained unanswered is how pathogenic fungi switch from vegetative growth to infection-related morphogenesis during a disease cycle. Here, we identify fungal oxylipin analogous the well-known phytohormone jasmonic acid, as principal signal responsible for such developmental pathogenicity in rice-blast fungus Magnaporthe oryzae . We explored molecular function(s) of intrinsic acid differentiation M. via OPR1 , which encodes 12-Oxo-phytodienoic Acid Reductase...

Aging is associated with altered mitochondrial function, which dependent on the magnesium (Mg+2 ) ion flux. The molecular mechanism underlying Mg+2 homeostasis, especially during aging has not been well understood. We previously demonstrated that absence of a vacuolar transporter Mnr2 accelerates cell death in older part colony Magnaporthe oryzae presumably due to an homeostasis. Here, we show localization as dynamic puncta at membrane, cells. Such are often localized close proximity...

Abstract Plant cell wall acts as a primary barrier for microbial pathogens during infection. A degrading enzyme thus may be crucial virulence factor, it aid the pathogen in successful host invasion. Nine genes coding feruloyl esterases (Fae), likely involved plant degradation, have been annotated genome of cereal-blast fungus Magnaporthe oryzae . However, role any Fae pathogenicity M. remains hitherto under explored. Here, we identified FAE1 gene (MGG_08737) that was significantly...

Fungal plant pathogens constantly evolve and deploy novel peptide metabolite effectors to break down resistance adapt new host plants. The blast fungal pathogen Pyricularia oryzae is a single species subdivided into multiple host-specific lineages. This specialization likely due secreted effectors, including effectors. Here, we mined 68 genomes of P. , belonging six lineages, identify secondary (SM) biosynthetic gene clusters (BGCs) associated with specialization. A similarity network...

Abstract Fungal plant pathogens constantly evolve and deploy novel peptide metabolite effectors to break down resistance adapt new host plants. The blast fungal pathogen Pyricularia oryzae is a single species subdivided into multiple host-specific lineages that have evolved through gain and/or loss of virulence effector related genes chromosomal rearrangement. Here, we mined 68 genomes P. , belonging six lineages, identify secondary (SM) biosynthetic gene clusters (BGCs) likely associated...

Abstract The outer kinetochore DASH/DAM complex ensures proper spindle structure and chromosome segregation. While DASH protein requirement diverges among different yeasts, its role in filamentous fungi has not been investigated so far. We studied the dynamics of middle (Mis12) (Dam1 Ask1) proteins fungal pathogen, Magnaporthe oryzae, which undergoes multiple cell cycle linked developmental transitions. Both Dam1 Ask1, unlike Mis12, were recruited to nucleus specifically during mitosis. was...

Abstract Aging is associated with altered mitochondrial function. Mitochondrial function dependent on the magnesium (Mg +2 ) ion flux. The molecular mechanism underlying Mg homeostasis, especially during aging has not been well understood. We previously demonstrated that absence of a vacuolar transporter Mnr2 accelerates cell death in older part colony Magnaporthe oryzae presumably due to an homeostasis. Localization as dynamic puncta at membrane cells further suggests its association aged...