A5054943017- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Plant Gene Expression Analysis
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Transgenic Plants and Applications
- Nitrogen and Sulfur Effects on Brassica
- Plant and animal studies
- Insect and Pesticide Research
- Microbial Metabolites in Food Biotechnology
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Biochemical and Molecular Research
- Plant biochemistry and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
Seoul National University
2012-2023
Vietnam National University Ho Chi Minh City
2020
Le Hong Phong High School for the Gifted
2020
Jeonbuk National University
2015
Seowon University
2007
Myongji University
2007
University of Georgia
1989-1995
Massachusetts General Hospital
1995
Methyl jasmonate is a plant volatile that acts as an important cellular regulator mediating diverse developmental processes and defense responses. We have cloned the novel gene JMT encoding S-adenosyl-l-methionine:jasmonic acid carboxyl methyltransferase (JMT) from Arabidopsis thaliana. Recombinant protein expressed in Escherichia coli catalyzed formation of methyl jasmonic with K(m) value 38.5 microM. RNA was not detected young seedlings but rosettes, cauline leaves, developing flowers. In...
Abstract AtMYB44 belongs to the R2R3 MYB subgroup 22 transcription factor family in Arabidopsis (Arabidopsis thaliana). Treatment with abscisic acid (ABA) induced transcript accumulation within 30 min. The gene was also activated under various abiotic stresses, such as dehydration, low temperature, and salinity. In transgenic carrying an promoter-driven β-glucuronidase (GUS) construct, strong GUS activity observed vasculature leaf epidermal guard cells. Transgenic overexpressing is more...
Summary Jasmonates play important roles in development, stress responses and defense plants. Here, we report the results of a study using functional genomics approach that identified rice basic helix‐loop‐helix domain gene, OsbHLH148 , conferred drought tolerance as component jasmonate signaling module rice. transcript levels were rapidly increased by treatment with methyl (MeJA) or abscisic acid, abiotic stresses including dehydration, high salinity, low temperature wounding. Transgenic...
The presence of a specific binding site for hepta-beta-glucoside elicitor phytoalexin accumulation has been demonstrated in soybean microsomal membranes. A tyramine conjugate the elicitor-active was prepared and radiolabeled with 125I. labeled hepta-beta-glucoside-tyramine used as ligand assays total membrane fraction from roots. Binding saturable, reversible, an affinity (apparent Kd = 7.5 x 10(-10) M) comparable concentration required biological activity. single class sites found....
The plastid transformation approach offers a number of unique advantages, including high-level transgene expression, multi-gene engineering, containment, and lack gene silencing position effects. extension technology to monocotyledonous cereal crops, rice, bears great promise for the improvement agronomic traits, efficient production pharmaceutical or nutritional enhancement. Here, we report promising step towards stable in rice. We produced fertile transplastomic rice plants demonstrated...
The abilities of a family chemically synthesized oligo-beta-glucosides, ranging in size from hexamer to decamer, induce phytoalexin accumulation soybean cotyledons were investigated determine which structural elements the oligoglucosides are important for their biological activity. results assays established that following motif is necessary oligo-beta-glucosides have high elicitor activity: [formula; see text] branched trisaccharide at nonreducing end was found be essential maximum...
The Arabidopsis thaliana transcription factor gene AtMYB44 was induced within 10 min by treatment with methyl jasmonate (MeJA). Wound-induced expression of the observed in local leaves, but not distal illustrating jasmonate-independent induction at wound sites. abolished mutants insensitive to (coi1), ethylene (etr1), or abscisic acid (abi3-1) when treated corresponding hormones. Moreover, various growth hormones and sugars also rapid transcript accumulation. Thus, activation appears be any...
Plants remember what they have experienced and are thereby able to confront repeated stresses more promptly strongly. A subset of the drought responsive genes, called stress memory displayed greatly elevated levels under recurrent conditions. To screen for a set genes in soybean (Glycine max L.), we designed 180K DNA chip comprising 60-bp probes synthesized situ examine 55,589 loci. Through microarray analysis using chip, identified 2,162 2,385 with than 4-fold increases or decreases...
ABSTRACT A DNA fragment encoding two enzymes leading to trehalose biosynthesis, maltooligosyltrehalose synthase (BvMTS) and trehalohydrolase (BvMTH), was cloned from the nonpathogenic bacterium Brevibacterium helvolum . The open reading frames for proteins are 2,331 1,770 bp long, respectively, overlap by four nucleotides. Recombinant BvMTS , BvMTH fusion gene BvMTSH constructed insertion of an adenylate in overlapping region, were expressed Escherichia coli Purified protein catalyzed...
Drought stress memory in plant can alter their physiological, biochemical and molecular to a subsequent stress. An experiment was conducted determine parameters of soybean seedlings under drought memory. 14-days-old were subjected three consecutive water deficit phases (D1, D2, D3), each phase recovered by re-watering (R1, R2, R3), control watering daily (R0). Leave from these collected analyzed. Significantly increasing contents soluble sugar proline observed at the first After re-water...
Tocopherols, essential components of the human diet, are synthesized exclusively by photosynthetic organisms. To increase tocopherol content increasing total flux to biosynthetic pathway, genes encoding Arabidopsis homogentisate phytyltransferase (HPT/VTE2) and cyclase (TC/VTE1) were constitutively overexpressed in lettuce (Lactuca sativa L.). Total transgenic plants overexpressing either was increased more than 2fold mainly due an γ-tocopherol. However, chlorophyll HPT/VTE2 TC/VTE1 lines...
Arabidopsis RNA polymerase II (RNAPII) C-terminal domain (CTD) phosphatases regulate stress-responsive gene expression and plant development via the dephosphorylation of serine (Ser) residues CTD.Some these (CTD phosphatase-like 1 (CPL1) to CPL3) negatively ABA stress responses.Here, we isolated AtCPL5, a cDNA encoding protein containing two CTD phosphatase domains (CPDs).• To characterize analyzed patterns subcellular localization, investigated various phenotypes AtCPL5overexpressors...
AtMYB44 is a member of the R2R3 MYB subgroup 22 transcription factors and regulates diverse cellular responses in Arabidopsis thaliana. We performed quadruple 9-merbased protein binding microarray (PBM) analysis, which revealed that full-size recognized bound to consensus sequence AACnG, where n represents A, G, C or T. The was confirmed by electrophoretic mobility shift assay (EMSA) with truncated containing N-terminal side domain. This result indicates domain alone sufficient exhibit...