A5077369234- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
- Physiological and biochemical adaptations
- Mosquito-borne diseases and control
- Neurobiology and Insect Physiology Research
- Immune Cell Function and Interaction
- Iron Metabolism and Disorders
- Malaria Research and Control
- Plant Molecular Biology Research
- Genetics, Aging, and Longevity in Model Organisms
Hanyang University
2017-2024
University of Pennsylvania
2024
Howard Hughes Medical Institute
2024
blood cells, called hemocytes, are classified into plasmatocytes, crystal and lamellocytes based on the expression of a few marker genes cell morphologies, which inadequate to classify complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes states involved cycle, antimicrobial response, metabolism together with identification intermediate states. Further, discovered rare...
Abstract The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis immunity. Three types hemocytes have characterized in gland: plasmatocytes, lamellocytes, crystal cells, which are analogous to vertebrate myeloid yet molecular underpinnings gland less investigated. Here, we use single-cell RNA sequencing comprehensively analyze heterogeneity developing discover...
Insect respiration has long been thought to be solely dependent on an elaborate tracheal system without assistance from the circulatory or immune cells
Drosophila hemocytes, like those of mammals, are given rise from two distinctive phases during both the embryonic and larval hematopoiesis. Embryonically derived mostly composed macrophage-like plasmatocytes, largely identified by genetic markers. However, cellular diversity distinct functions possible subpopulations within plasmatocytes have not been explored in larvae. Here, we show that exhibit differential expressions Hemolectin (Hml) Peroxidasin (Pxn) development. Moreover, removal...
Drosophila hemocytes are akin to mammalian myeloid blood cells that function in stress and innate immune-related responses. A multi-potent progenitor population responds local signals systemic by expanding the number of functional cells. Here we show mechanisms demonstrate an integration environmental carbon dioxide (CO2) oxygen (O2) inputs initiate a cascade signaling events, involving multiple organs, as response when levels these two important respiratory gases fall below threshold. The...
Drosophila hemocytes serve as the primary defense system against harmful threats, allowing animals to thrive. Hemocytes are often compared vertebrate innate immune cells due observed functional similarities between two. However, have primarily been established based on a limited number of genes and their homologies. Thus, systematic analysis using transcriptomic data could offer novel insights into hemocyte function provide new perspectives evolution system. Here, we performed cross-species...
Iron is an essential divalent ion for aerobic life. Life has evolved to maintain iron homeostasis normal cellular and physiological functions therefore imbalances in levels exert a wide range of consequences. Responses dysregulation blood development, however, remain elusive. Here, we found that critical differentiation Drosophila cells the larval hematopoietic organ, called lymph gland. Supplementation chelator, bathophenanthroline disulfate (BPS) results excessive crystal cell This...
Summary Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal and lamellocytes based on the expression of a few marker genes cell morphologies, which inadequate to classify complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes states involved cycle, antimicrobial response, metabolism together with identification intermediate states. Further,...
Abstract The nuclear factor binding the κ light chain in B-cells (NFκB) is involved a wide range of cellular processes including development, growth, innate immunity, and sleep. However, genetic studies role specific NFκB transcription factors sleep have been limited. Drosophila fruit flies carry three genes encoding factors, Dorsal, Dorsal Immunity Factor (Dif), Relish. We previously found that loss Relish gene from fat body suppressed daily nighttime sleep, abolished infection-induced Here...
SUMMARY Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis immunity. Three types mature hemocytes have characterized in gland: plasmatocytes, lamellocytes, crystal cells, which are analogous to vertebrate myeloid cells. Here, we used single-cell RNA sequencing comprehensively analyze heterogeneity developing discovered novel hemocyte types, stem-like prohemocytes,...
Summary The Drosophila lymph gland houses blood progenitors that give rise to myeloid-like cells. Initially, proliferate, but later become quiescent maintain multipotency before differentiation. Despite the identification of various factors involved in maintenance, cellular mechanism regulating progenitor quiescence remains elusive. Here, we reveal expression nitric oxide synthase progenitors, generating for post-translational S-nitrosylation protein cysteine residues. activates...
Drosophila hemocytes serve as the primary defense system required for animals to thrive and overcome harmful threats. These cells are often compared of vertebrate innate immune due observed functional similarities between two. However, these have been primarily established based on a limited number genes. Thus, systematic analysis using transcriptomic data could offer novel insights into hemocyte function at level provide new perspectives evolution system. Here, we performed cross-species...