A5071874745- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- MicroRNA in disease regulation
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Metal-Catalyzed Oxygenation Mechanisms
- Microtubule and mitosis dynamics
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Redox biology and oxidative stress
- Ubiquitin and proteasome pathways
- Porphyrin Metabolism and Disorders
- Biochemical and Molecular Research
- Peptidase Inhibition and Analysis
- Plant nutrient uptake and metabolism
- Natural product bioactivities and synthesis
- Library Science and Administration
- Muscle Physiology and Disorders
- Cancer-related Molecular Pathways
- Cellular transport and secretion
- HIV Research and Treatment
- Fungal and yeast genetics research
- Cellular Mechanics and Interactions
Korea University
2008-2024
The University of Texas Southwestern Medical Center
2016-2023
Southwestern Medical Center
2023
Korea Basic Science Institute
2023
Korea Institute of Science and Technology
2023
Korean Association Of Science and Technology Studies
2023
Friedrich Miescher Institute
2023
Okinawa Institute of Science and Technology Graduate University
2023
Gwangju Institute of Science and Technology
2023
Abstract Plant thioredoxins (Trxs) participate in two redox systems found different cellular compartments: the NADP-Trx system (NTS) cytosol and mitochondria ferredoxin-Trx (FTS) chloroplast, where they function as regulators by regulating activity of various target enzymes. The identities master that maintain homeostasis modulate timed development through have remained completely unknown. Here, we show proteins consisting a single cystathionine β-synthase (CBS) domain pair stabilize plant...
Abstract Defects in plasma membrane repair can lead to muscle and heart diseases humans. Tripartite motif-containing protein (TRIM)72 (mitsugumin 53; MG53) has been determined rapidly nucleate vesicles at the site of damage, but underlying molecular mechanisms remain poorly understood. Here we present structure Mus musculus TRIM72, a complete model TRIM E3 ubiquitin ligase. We demonstrated that interaction between TRIM72 phosphatidylserine-enriched membranes is necessary for its oligomeric...
MicroRNAs regulate the expression of many proteins and require specific maturation steps. Primary microRNA transcripts (pri-miRs) are cleaved by Microprocessor, a complex containing RNase Drosha its partner protein, DGCR8. Although DGCR8 is known to bind heme, molecular role heme in pri-miR processing unknown. Here we show that critical for Microprocessor process pri-miRs with high fidelity. Furthermore, degree inherent dependence varies different pri-miRs. Heme-dependent fail properly...
Abstract The spindle checkpoint maintains genomic stability and prevents aneuploidy. Unattached kinetochores convert the latent open conformer of protein Mad2 (O-Mad2) to active closed (C-Mad2), bound Cdc20. C-Mad2–Cdc20 is incorporated into mitotic complex (MCC), which inhibits anaphase-promoting complex/cyclosome (APC/C). C-Mad2-binding p31 comet ATPase TRIP13 promote MCC disassembly silencing. Here, using nuclear magnetic resonance (NMR) spectroscopy, we show that catalyze conversion...
Anther formation and dehiscence are complex pivotal processes in reproductive development. The secondary wall thickening endothecial cells of the anther is a known prerequisite for successful dehiscence. However, many gaps remain our understanding regulatory mechanisms underlying planta, including possible role jasmonic acid (JA) H(2)O(2) cells. Here, we report that cystathionine β-synthase domain-containing protein CBSX2 located chloroplast plays critical cell walls endothecium during...
Cystathione beta-synthase domain-containing protein 2 (CDCP2) from Arabidopsis thaliana has been overexpressed and purified to homogeneity. As an initial step towards three-dimensional structure determination, crystals of recombinant CDCP2 have obtained using polyethylene glycol 8000 as a precipitant. The diffracted 2.4 A resolution synchrotron radiation belonged the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters = b 56.360, c 82.596 A, alpha beta 90, gamma 120 degrees ....
Ski7 (superkiller protein 7) plays a critical role in the mRNA surveillance pathway. The C-terminal fragment of (residues 520–747) from Saccharomyces cerevisiae was heterologously expressed Escherichia coli and purified to homogeneity. It successfully crystallized preliminary X-ray data were collected 2.0 Å resolution using synchrotron radiation. crystal belonged trigonal space group, either P 3 1 21 or 2 21, with unit-cell parameters = b 73.5, c 83.6 Å. asymmetric unit contains one molecule...
The nitrilase superfamily, including 13 branches, plays various biological functions in signaling molecule synthesis, vitamin metabolism, small-molecule detoxification, and posttranslational modifications. Most of the mammals yeasts have Nit1 Nit2 proteins, which belong to nitrilase-like (Nit) branch superfamily. Recent studies suggested that is a metabolite repair enzyme, whereas shows ω-amidase activity. In addition, are as putative tumor suppressors through different ways mammals. Yeast...
Abstract Plasma membrane repair defects cause human muscle and heart diseases. In the early stage of repair, TRIM72/MG53 nucleates vesicles to damaged site within seconds. Despite therapeutic potential TRIM72/MG53, molecular mechanisms are poorly understood. Here, we present structure TRIM72, first complete model a TRIM E3 ligase. Structure-guided functional analysis revealed unique phospholipid recognition mode in which pair PRYSPRY domains interacts with negatively charged membranes....
Streptococcus pneumoniae is the major cause of pneumonia, meningitis and septicemia [1], [2].Pneumococcal infections are a global health problem worldwide.The search novel combat strategies urgently needed.Phosphoglycerate kinase (PGK) from S. enzyme in gycolytic pathway, also important as surface protein.We have solved structure PGK pneumoniae.Further investigations will reveal role virulence factor.This information could be relevant fight against infection diseases caused by pneumococcus
SessionsC635 of the oligonucleotide in presence a variety metal ions [2] (Mg 2+ , Mn Ca Ni Sr Ba Cd Cu Co(NH 3 ) 6 3+ Zn y Li + to stabilize structure crystal.By X-ray diffraction we obtained patterns with low resolution from which fi rst stage, it was only possible determine cell parameters and space group.The best crystals were cadmium has high affi nity for adenine bases.Contrary common behavior A-T rich oligonucleotides, when hairpin been stabilized are at 21°C, while using other...