A5003753354- Advanced Electron Microscopy Techniques and Applications
- Connexins and lens biology
- Enzyme Structure and Function
- Electron and X-Ray Spectroscopy Techniques
- Mass Spectrometry Techniques and Applications
- Receptor Mechanisms and Signaling
- Supramolecular Self-Assembly in Materials
- interferon and immune responses
- Ion Channels and Receptors
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Molecular Communication and Nanonetworks
- Bacterial Genetics and Biotechnology
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Advanced Fluorescence Microscopy Techniques
- Genomics and Phylogenetic Studies
- Quantum Dots Synthesis And Properties
- Heat shock proteins research
- Escherichia coli research studies
- ATP Synthase and ATPases Research
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- melanin and skin pigmentation
- RNA and protein synthesis mechanisms
Korea Basic Science Institute
2015-2024
Chonnam National University Hospital
2024
Korea University
2020-2023
Korea Institute of Science and Technology
2023
Korean Association Of Science and Technology Studies
2023
Friedrich Miescher Institute
2023
Okinawa Institute of Science and Technology Graduate University
2023
Gwangju Institute of Science and Technology
2023
The University of Texas Southwestern Medical Center
2023
Southwestern Medical Center
2023
Cryo-EM structure of human connexin 31.3 hemichannel reveals the selective permeability mechanism.
Neuropeptide Y (NPY) is highly abundant in the brain and involved various physiological processes related to food intake anxiety, as well human diseases such obesity cancer. However, molecular details of interactions between NPY its receptors are poorly understood. Here, we report a cryo-electron microscopy structure NPY-bound neuropeptide Y1 receptor (Y1R) complex with Gi1 protein. The C-terminal segment forming extended conformation binds deep into Y1R transmembrane core, where amidated...
Abstract Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). We report cryo-electron microscopy structures of Cx43 GJICh, revealing dynamic equilibrium state various channel conformations detergents and lipid nanodiscs. identify three different N-terminal helix Cx43—gate-covering (GCN), pore-lining (PLN), flexible intermediate (FIN)—that are randomly...
Connexin 36 (Cx36) is responsible for signal transmission in electrical synapses by forming interneuronal gap junctions. Despite the critical role of Cx36 normal brain function, molecular architecture junction channel (GJC) unknown. Here, we determine cryo-electron microscopy structures GJC at 2.2-3.6 Å resolutions, revealing a dynamic equilibrium between its closed and open states. In state, pores are obstructed lipids, while N-terminal helices (NTHs) excluded from pore. state with...
G-protein coupled receptors (GPCRs) and ion channels serve as key molecular switches through which extracellular stimuli are transformed into intracellular effects, it has long been postulated that direct effector molecules of the alpha subunit G-proteins (Gα). However, no complete structural evidence supporting interaction between Gα is available. Here, we present cryo-electron microscopy structures human transient receptor potential canonical 5 (TRPC5)-Gα
p62/SQSTM1 is the key autophagy adapter protein and hub of multi-cellular signaling. It was recently reported that N-end rule pathways are linked via p62. However, exact recognition mode degrading substrates regulation p62 in autophagic pathway remain unknown. Here, we present complex structures between ZZ-domain various type-1 type-2 N-degrons. The binding employed interaction with N-degrons differs from by classic N-recognins. also determined oligomerization PB1 domain can control...
Escherichia coli AcrAB-TolC is a multidrug efflux pump that expels wide range of toxic substrates. The dynamic nature the binding or low affinity between components has impeded elucidation how three assemble in functional state. Here, we created fusion proteins composed AcrB, transmembrane linker, and two copies AcrA. protein exhibited acridine pumping activity, suggesting reflects structure vivo. To discern assembling mode with TolC, AcrBA was incubated TolC chimeric containing aperture tip...
Abstract Defects in plasma membrane repair can lead to muscle and heart diseases humans. Tripartite motif-containing protein (TRIM)72 (mitsugumin 53; MG53) has been determined rapidly nucleate vesicles at the site of damage, but underlying molecular mechanisms remain poorly understood. Here we present structure Mus musculus TRIM72, a complete model TRIM E3 ubiquitin ligase. We demonstrated that interaction between TRIM72 phosphatidylserine-enriched membranes is necessary for its oligomeric...
Abstract Single‐molecule techniques are powerful microscopy methods that provide new insights into biological processes. Liquid‐phase transmission electron (LP‐TEM) is an ideal single‐molecule technique for overcoming the poor spatiotemporal resolution of optical approaches. However, LP‐TEM limited by several challenges such as electron‐beam‐induced molecular damage, difficulty in identifying biomolecular species, and a lack analytical approaches conformational dynamics. Herein, graphene...
The stressosome transduces environmental stress signals to SigB upregulate SigB-dependent transcription, which is required for bacterial viability. core composed of RsbS and at least one the RsbR paralogs. A previous cryo-electron microscopy (cryo-EM) structure RsbRA–RsbS complex determined under a D 2 symmetry restraint showed that forms pseudo-icosahedron consisting 60 STAS domains RsbRA RsbS. However, it still unclear how paralogs assemble into stressosome. Here, an assembly model...
Molecular switches alterable between two stable states by environmental stimuli, such as light and temperature, offer the potential for controlling biological functions. Here, we report a circular photoswitchable protein complex made of multiple molecules that can rapidly reversibly switch with significant conformational changes. The structural photochromic properties photoactive yellow (PYP) are harnessed to construct oligomer PYPs (coPYPs) desired sizes. Considering light-induced...
Abstract In biology, evolutionary conserved protein sequences show homologous physiological phenotypes in their structures and functions. If the has a vital function, its sequence is usually across species. However, highly there still remains small differences Upon protein–protein interaction (PPI), it observed that proteins can have different binding partners are considered to be caused by variations specific domain. Thyroglobulin (TG) most commonly found thyroid gland of vertebrates serves...
Manipulation and control of cell chemotaxis remain an underexplored territory despite vast potential in various fields, such as cytotherapeutics, sensors, even robots. Herein is achieved the chemical over chemotactic movement direction Jurkat T cells, a representative model, by construction cell-in-catalytic-coat structures single-cell nanoencapsulation. Armed with catalytic power glucose oxidase (GOx) artificial coat, nanobiohybrid cytostructures, denoted Jurkat[Lipo_GOx] , exhibit...
Abstracts Background Transmission electron microscopy (TEM) is an excellent tool for studying detailed biological structures. High-resolution structure determination now routinely performed using advanced sample preparation techniques and image processing software. In particular, correction contrast transfer function (CTF) crucial extracting high-resolution information from TEM that convoluted by imperfect imaging condition. Accurate of defocus, one the major elements constituting CTF,...
Cryo-electron microscopy (cryo-EM) is now the first choice to determine high-resolution structures of huge protein complexes. Grids with two-dimensional arrays holes covered a carbon film are typically used in cryo-EM. Although semi-automatic plungers available, notable trial-and-error still required obtain suitable grid specimen. Herein, we introduce new method thin ice specimens using real-time measurement liquid amounts cryo-EM grids. The grids for strongly diffracted laser light, and...
Abstract The selection of defocus ranges for small datasets in cryo-electron microscopy (cryo-EM) is under-researched. We present a comprehensive benchmark experiment that aimed to evaluate the relationship between contrast, defocus, and resolution, particularly context limited datasets. conducted detailed analysis beta-galactosidase, apo-ferritin, connexin-46/50 optimize pre-screening strategies cryo-EM. Our approach involved classifying micrographs based on image contrast using an...
<h3>Background:</h3> Autoimmune inflammatory rheumatic diseases (AIIRDs) are systemic conditions marked by immune responses against self-antigens, exhibiting diverse clinical manifestations and heterogeneous pathophysiology among patients. Despite this variability, the lack of personalized diagnostics therapies remains a challenge. Immunophenotyping has been widely used various experimental methods, offering dynamic quantitative insight into immunological status individual...