A5079916648- Bacterial Genetics and Biotechnology
- Nuclear Structure and Function
- Enzyme Structure and Function
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Redox biology and oxidative stress
- RNA Research and Splicing
- Cancer Research and Treatments
- DNA Repair Mechanisms
- Trace Elements in Health
- Sulfur Compounds in Biology
- Genomics and Chromatin Dynamics
- Salmonella and Campylobacter epidemiology
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Chemical Reactions and Isotopes
- Aquaculture disease management and microbiota
- Escherichia coli research studies
- Endoplasmic Reticulum Stress and Disease
- Muscle activation and electromyography studies
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
- Biofuel production and bioconversion
- Stroke Rehabilitation and Recovery
- Bacteriophages and microbial interactions
Korea Research Institute of Chemical Technology
2022-2024
Seoul National University
2014-2023
Pusan National University
2014
Significance In gram-negative bacteria, OxyR is the master peroxide sensor that regulates transcription of defense genes in response to a low level cellular H 2 O via rapid kinetic reaction. this study, we present first, our knowledge, full-length structures peroxide-sensing regulator together with an oxidation intermediate-mimicking structure. The show all structural features describing tetrameric assembly and bound molecule near conserved cysteine. Combining results, reveal step-by-step...
Abstract Nuclear structure and function are governed by lamins, which intermediate filaments that mostly consist of α-helices. Different lamin assembly models have been proposed based on low resolution fragmented structures. However, their mechanisms still poorly understood at the molecular level. Here, we present crystal a long human fragment 3.2 Å allows visualization features full-length protein. The shows an anti-parallel arrangement two coiled-coil dimers, is important for process. We...
Hypochlorous acid (HOCl) is generated in the immune system to kill microorganisms. In Escherichia coli , a hypochlorite-specific transcription regulator, HypT, has been characterized. HypT belongs LysR-type transcriptional regulator (LTTR) family that contains DNA-binding domain (DBD) and regulatory (RD). Here, we identified hypT gene from Salmonella enterica serovar Typhimurium determined crystal structures of full-length protein RD. The structure reveals type tetrameric assembly LTTR...
Abstract The COVID‐19 pandemic has required an expeditious advancement of innovative antiviral drugs. In this study, focused compound libraries are synthesized in 96‐ well plates utilizing modular click chemistry to rapidly discover potent inhibitors targeting the main protease (M pro ) SARS‐CoV‐2. Subsequent direct biological screening identifies novel 1,2,3‐triazole derivatives as robust M with high anti‐SARS‐CoV‐2 activity. Notably, C5N17B demonstrates sub‐micromolar inhibitory potency...
In response to microbial invasion, the animal immune system generates hypochlorous acid (HOCl) that kills microorganisms in oxidative burst. HOCl toxicity is amplified phagosome through import of copper cation (Cu2+). Escherichia coli and Salmonella, transcriptional regulator RclR senses stress induces expression RclA, -B, -C proteins involved bacterial defenses against stress. However, structures biochemical roles Rcl remain be elucidated. this study, we first examined role flavoprotein...
Nuclear lamins maintain the nuclear envelope structure by forming long linear filaments via two alternating molecular arrangements of coiled-coil dimers, known as A11 and A22 binding modes. The mode is characterized antiparallel interactions between coil 1b domains, whereas facilitated 2 domains lamin. junction A11- A22-interacting dimers in lamin tetramer produces another parallel head-tail interaction 1a C-terminal region 2, called ACN interaction. During mitosis, phosphorylation...
In this paper, design of a wearable hand exoskeleton system for exercising flexion/extension the fingers, is proposed. The was designed with simple and structure to aid finger motions in 1 degree freedom (DOF). A grasping experiment by fully-abled people performed investigate general polynomial curve obtained. To customize user, adjusted joint range motion (ROM) user optimal obtained using optimization algorithm. prototype divided into two parts (one part thumb, other rest fingers) actuated...
Salmonella enterica serovar Typhimurium (S. Typhimurium) is an intracellular pathogen that has evolved to survive in the phagosome of macrophages. The periplasmic copper-binding protein CueP was initially known confer copper resistance S. Typhimurium. Crystal structure and biochemical studies on revealed a putative binding site surrounded by conserved cysteine histidine residues. A recent study reported supplies ions Cu, Zn-superoxide dismutase (SodCII) at low concentration thus enables...
Intermediate filaments (IFs) commonly have structural elements of a central α-helical coiled-coil domain consisting coil 1a, 1b, 2, and their flanking linkers. Recently, the crystal structure long lamin A/C fragment was determined showed detailed features tetrameric unit. The further suggested new binding mode between tetramers, designated eA22, where parallel overlap 1a 2 is critical interaction. This study investigated biochemical effects genetic mutations causing human diseases, focusing...
In this paper, a hand exoskeleton system for virtual reality is proposed. As interface the hand, wearable should be able to measure finger joint angles and apply force feedback fingers with simple light design. proposed system, are measured by tendon-inspired cable mechanism. Also, another used finger. Using motor currents, cable-driven actuation applies desired fingers. That is, when zero, position controlled track changed length; needs applied, current generate force. For smooth transition...
Fusobacterium nucleatum is a lesion-associated obligate anaerobic pathogen of destructive periodontal disease; it also implicated in the progression and severity colorectal cancer. Four genes (FN0625, FN1055, FN1220, FN1419) F. are involved producing hydrogen sulfide (H2S), which plays an essential role against oxidative stress. The molecular functions Fn1419 known, but their mechanisms remain unclear. We determined crystal structure at 2.5 Å, showing unique conformation PLP-binding site...
The transcriptional activator AphB has been implicated in acid resistance and pathogenesis the food borne pathogens Vibrio vulnificus cholerae.To date, full-length crystal structure of V. cholerae determined characterized by a tetrameric assembly consisting DNA binding domain regulatory (RD).Although acidic pH low oxygen tension might be involved activation AphB, it remains unknown which ligand or stimulus activates at molecular level.In this study, we determine RD from under aerobic...
The bacterial transcriptional regulator OxyR is known to function as a two-state redox switch. senses cellular levels of H2O2 via "sensing cysteine" that switches from the reduced disulfide state upon exposure, inducing expression antioxidant genes. and states OxyR, respectively, bind extended compact regions DNA, where blocks oxidized allows transcription further induces target gene by interacting with RNA polymerase. Vibrio vulnificus OxyR2 high sensitivity encoding Prx2. In this study, we...
Most Gram-negative bacteria respond to excessive levels of H2O2 using the peroxide-sensing transcriptional regulator OxyR, which can induce expression antioxidant genes restore normality. Vibrio vulnificus has two distinct OxyRs (OxyR1 and OxyR2), are sensitive different peroxidases, Prx1 Prx2. Although OxyR1 both high sequence similarity sensitivity comparable with that other OxyR proteins, OxyR2 exhibits limited is more To investigate basis for this difference, we determined crystal...
The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa , has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as activator of the MexEF-OprN system. MexT consists an N-terminal DNA-binding domain and C-terminal regulatory (RD). Little is known regarding ligands its mechanism activation. We elucidated crystal structure RD at 2.0 Å resolution. comprised two protomer chains in dimeric arrangement. possessed arginine-rich region hydrophobic patch...
The nucleoskeletal protein lamin is primarily responsible for the mechanical stability of nucleus.The assembly process requires A11, A22, and ACN binding modes coiled-coil dimers.Although X-ray crystallography chemical cross-linking analysis A/C have provided snapshots A11 modes, mechanism entire filament remains to be explained.Here, we report a crystal structure coil 2 fragment, revealing A22 interaction at atomic resolution.The showed detailed structural features, indicating that two...
Macrophages produce itaconic acid in phagosomes response to bacterial cell wall component lipopolysaccharide eliminate invading pathogenic bacteria. Itaconic competitively inhibits the first enzyme of glyoxylate cycle. To overcome stress, bacteria employ LysR-type transcriptional regulator RipR. However, it remains unknown which molecule activates RipR pathogenesis. In this study, we determined crystal structure regulatory domain from intracellular pathogen Salmonella. The exhibited typical...
Nanoscopic investigation of bacterial cells is essential to reveal their physiological status, impacting all cellular functions. Currently, this requires labeled probes or targeted staining procedures. Herein, we report a new feature, intracellular dynamics-resolved Rayleigh scattering (IDRS), that visualizes spatiotemporal cytoplasmic transitions in unlabeled bacteria and characterizes real-time status 10 s. From single-bacterium IDRS signals, discovered unique spatial patterns multiple...
LysR-type transcriptional regulators (LTTRs) contain an N-terminal DNA binding domain (DBD) and a C-terminal regulatory (RD). Typically, LTTRs function as homotetramers. VV2_1132 was identified in
Lamins are intermediate filaments that form a 3-D meshwork in the periphery of nuclear envelope. The recent crystal structure long fragment human lamin A/C visualized tetrameric assembly unit central rod domain as polymerization intermediate. A genetic mutation S143F caused phenotype characterized by both progeria and muscular dystrophy. In this study, we determined harboring mutation. obtained revealed X-shaped interaction between units crystals, potentiated hydrophobic interactions mutated...