A5018742701- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Biochemical and Molecular Research
- Advanced Fluorescence Microscopy Techniques
- Drug Transport and Resistance Mechanisms
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Cancer Research and Treatments
- Porphyrin Metabolism and Disorders
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Protein Structure and Dynamics
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Cancer-related Molecular Pathways
- Microbial Community Ecology and Physiology
- Sulfur Compounds in Biology
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Cancer Cells and Metastasis
- Enzyme Production and Characterization
Dalian Minzu University
2016-2025
Inner Mongolia University of Science and Technology
2025
Minzu University of China
2017-2024
Sichuan Agricultural University
2024
Chinese Academy of Sciences
2015-2023
Dalian Institute of Chemical Physics
2015-2023
Qingdao University
2022
Affiliated Hospital of Qingdao University
2022
State Ethnic Affairs Commission
2013-2014
Zhejiang Marine Fisheries Research Institute
2013
Differentially from other kinds of Ras, oncogenic K-Ras, which is mutated approximately 30% human cancer, does not induce apoptosis and senescence. Here, we provide the evidence that K-Ras abrogates p53 function expression through induction Ataxia telangiectasia-mutated Rad3-related mediated Snail stabilization. directly binds to DNA binding domain diminishes tumor-suppressive p53. Thus, elimination si-RNA can in K-Ras-mutated cells, whereas mutant suppress regardless status. Chemicals,...
Interleukin-17 receptor A (IL17RA) is a critical mediator of pro-inflammatory cytokine signaling and key immune checkpoint in autoimmune diseases. While monoclonal antibodies targeting IL17RA have demonstrated clinical efficacy, their high costs, complexity production, lack oral bioavailability present significant limitations. In response to these challenges, we developed AL-8(0), novel peptide specifically designed inhibit the IL17A-IL17RA pathway. AL-8(0) was synthesized with purity...
Fluorescent proteins (FPs) are widely used as optical probes in molecular and cell biology. tKeima is a tetrameric, large Stokes shift red fluorescent protein the ancestral of mt-Keima, which applied pH-sensitive probe. While pH sensitivity mt-Keima well characterized, pH-dependent properties have not been comprehensively elucidated. To obtain better understanding effects on tKeima, its emission intensity at various levels was measured, crystal structure 4.0 determined resolution 2.2 Å. The...
Macrolide-specific efflux pump MacAB-TolC has been identified in diverse Gram-negative bacteria including Escherichia coli. The inner membrane transporter MacB requires the outer factor TolC and periplasmic adaptor protein MacA to form a functional tripartite complex. In this study, we used chimeric containing tip region of α-barrel investigate role with regard its interaction MacA. formed stable complex MacA, formation was abolished by substitution at functionally essential residues located...
MacB is a noncanonic ABC-type transporter within Gram-negative bacteria, which responsible both for the efflux of macrolide antibiotics and secretion heat-stable enterotoxin II. In Escherichia coli, requires membrane fusion protein MacA multifunctional outer channel TolC to pump substrates external medium. Sequence analysis suggested that has relatively large periplasmic region. To gain insight into how assembles with TolC, we determined crystal structure region Actinobacillus...
Sensitive and accurate detection of specific metal ions is important for sensor development can advance analytical science support environmental human medical examinations. Fluorescent proteins (FPs) be quenched by spectroscopically show a unique fluorescence-quenching sensitivity, suggesting their potential application as FP-based biosensors. Since the characteristics fluorescence quenching are difficult to predict, spectroscopic analysis new FPs Here we reported structural metal-induced...
The Q-426 strain isolated from compost samples has excellent antifungal activities against a variety of plant pathogens. However, the complete genome is still unclear, which limits potential application Q-426.Genome sequencing revealed that contains single circular chromosome 4,086,827 bp in length, with 4691 coding sequences and an average GC content 46.3%. high degree collinearity B. velezensis FZB42, SQR9, amyloliquefaciens DSM7, was reidentified as based on homology analysis results....
Fusobacterium nucleatum is a lesion-associated obligate anaerobic pathogen of destructive periodontal disease; it also implicated in the progression and severity colorectal cancer. Four genes (FN0625, FN1055, FN1220, FN1419) F. are involved producing hydrogen sulfide (H2S), which plays an essential role against oxidative stress. The molecular functions Fn1419 known, but their mechanisms remain unclear. We determined crystal structure at 2.5 Å, showing unique conformation PLP-binding site...
Staphylococcus aureus AgrC is an important component of the agr quorum-sensing system. a membrane-embedded histidine kinase that thought to act as sensor for recognition environmental signals and transduction into cytoplasm. However, difficulty expressing purifying functional membrane proteins has drastically hindered in-depth understanding molecular structures physiological functions these proteins. Here, we describe high-yield expression purification AgrC, analyze its activity. A...
The Keima family comprises large Stokes shift fluorescent proteins that are useful for dual-color fluorescence cross-correlation spectroscopy and multicolor imaging. tKeima is a tetrameric protein serves as the ancestor both dKeima mKeima. spectroscopic properties of have been previously reported; however, its structural basis molecular not yet elucidated. In this study, we present crystallographic results tKeima. purified spontaneously crystallized after purification without further...
Periplasmic membrane-fusion proteins (MFPs) are an essential component of the multidrug efflux pump in Gram-negative bacteria. They play a crucial role bridging outer membrane porin TolC and two distinct types inner transporters. The MFP MacA bridges ABC-type transporter MacB TolC. from pathogenic bacterium Actinobacillus actinomycetemcomitans was expressed Escherichia coli B834 (DE3) recombinant protein purified using Ni–NTA affinity, Q anion-exchange gel-filtration chromatography....
Trichlorobenzene isomers with different molecular structures (1,2,3-, 1,2,4- and 1,3,5-trichlorobenzene) were used as shape luminescence tuners of C70 nano/microcrystals. nano/microcrystals morphologies (nano/microrods, nano/microsheet clusters nano/microballs) synthesized. nano/microballs fabricated using 1,3,5-trichlorobenzene have a simple cubic (sc) structure, obtained other solvents face-centered-cubic (fcc) structure. Meanwhile, the introduction trichlorobenzenes in enhanced their PL...
Fluorescent proteins exhibit fluorescence quenching by specific transition metals, suggesting their potential as fluorescent protein-based metal biosensors. Each protein exhibits unique spectroscopic properties and mechanisms for metals. Therefore, the metal-induced analysis of various new would be important step towards development such Here, we first report structural yellow ZsYellow, following its quenching. Spectroscopic showed that ZsYellow exhibited a high degree Cu2+. During...
The universal stress proteins (USP) family member UspE is a tandem-type USP that consists of two Usp domains. expression levels the Escherichia coli (E. coli) become elevated in response to oxidative and DNA damaging agents, including exposure mitomycin C, cadmium, hydrogen peroxide. It has been shown UspA members are survival factors during cellular growth arrest. structures functions control E. animal hosts. While several have known structures, structure remains be elucidated.To understand...