A5058472231- Amino Acid Enzymes and Metabolism
- Sulfur Compounds in Biology
- Folate and B Vitamins Research
- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Bacteriophages and microbial interactions
- Amyotrophic Lateral Sclerosis Research
- Clostridium difficile and Clostridium perfringens research
- Ga2O3 and related materials
- Oral microbiology and periodontitis research
- Polyamine Metabolism and Applications
- Neurological diseases and metabolism
- Bacterial Genetics and Biotechnology
- Redox biology and oxidative stress
- Cholinesterase and Neurodegenerative Diseases
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Advanced X-ray Imaging Techniques
- Prion Diseases and Protein Misfolding
Seoul National University
2019-2025
Biocon (Switzerland)
2023
Zero to Three
2023
The formation of superoxide dismutase 1 (SOD1) filaments has been implicated in amyotrophic lateral sclerosis (ALS). Although the disulfide bond formed between Cys57 and Cys146 active state well studied, role reduced cysteine residues, Cys6 Cys111, SOD1 filament remains unclear. In this study, we investigated residues by determining comparing cryoelectron microscopy (cryo-EM) structures wild-type (WT) C6A/C111A under thiol-based reducing metal-depriving conditions, starting with protein...
Bacillus cereus, a Gram-positive aerobic bacterium commonly found in soil, food, and water, forms endospores that can withstand harsh environmental conditions. The are encased protective spore coat consisting of multiple layers proteins, among which, CotE serves as crucial morphogenetic protein within the outer coat. In this study, we observed homotrimeric underwent further oligomerization induced by Ca2+ was subsequently dissociated dipicolinic acid, compound released from core during...
Abstract Eukaryotic Cu, Zn-superoxide dismutase (SOD1) is primarily responsible for cytotoxic filament formation in amyotrophic lateral sclerosis (ALS) neurons. Two cysteine residues SOD1 form an intramolecular disulfide bond. This study aims to explore the molecular mechanism of by overoxidation sporadic ALS (sALS). In this study, we determined crystal structure double mutant (C57D/C146D) that mimics disulfide-forming residues. The revealed open and relaxed conformation loop IV containing...
Many enzymes require pyridoxal 5’-phosphate (PLP) as an essential cofactor and share active site residues in mediating diverse enzymatic reactions. Methionine can be converted into cysteine by cystathionine γ-lyases (CGLs) through a transsulfuration reaction dependent on PLP. In bacteria, MccB, also known YhrB, exhibits CGL activity that cleaves the C–S bond of at γ position. this study, we determined crystal structure MccB from Staphylococcus aureus its apo- PLP-bound forms. The structures...
Cryo-electron microscopy (cryo-EM) is now the first choice to determine high-resolution structures of huge protein complexes. Grids with two-dimensional arrays holes covered a carbon film are typically used in cryo-EM. Although semi-automatic plungers available, notable trial-and-error still required obtain suitable grid specimen. Herein, we introduce new method thin ice specimens using real-time measurement liquid amounts cryo-EM grids. The grids for strongly diffracted laser light, and...
The formation of uniform vitreous ice is a crucial step in the preparation samples for cryogenic electron microscopy (cryo-EM). Despite rapid technological progress EM, controlling thickness on sample grids with reproducibility remains major obstacle to obtaining high-quality data cryo-EM imaging. commonly employed classical blotting process faces problem excess water that cannot be absorbed by filter paper, resulting thick and heterogeneous ice. In this study, we propose novel approach...
The HlyBD-TolC complex, belonging to the type I secretion system (T1SS), is found in many pathogenic Gram-negative bacteria.This complex comprises three components: ABC-type inner membrane transporter HlyB, periplasmic adaptor HlyD, and outer channel TolC.Recent crystal cryo-EM structures have shed light on stoichiometry of HlyB hexamer HlyD functional complex.In this study, we performed a modeling study using AI-based structure prediction program Alphafold-multimer gain deeper understanding...