A5011729658- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Poxvirus research and outbreaks
- Biochemical and Structural Characterization
- RNA modifications and cancer
- Plant Virus Research Studies
- Electron and X-Ray Spectroscopy Techniques
- HIV Research and Treatment
- Virus-based gene therapy research
- Animal Virus Infections Studies
- interferon and immune responses
- Chemical Synthesis and Analysis
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Antibiotic Resistance in Bacteria
- Extracellular vesicles in disease
- Toxin Mechanisms and Immunotoxins
- Heat shock proteins research
- Protein Structure and Dynamics
- Supramolecular Self-Assembly in Materials
- Molecular Biology Techniques and Applications
Sungkyunkwan University
2023-2025
Okinawa Institute of Science and Technology Graduate University
2020-2023
Korea Basic Science Institute
2013-2023
The University of Texas Southwestern Medical Center
2023
Korea Institute of Science and Technology
2023
Korean Association Of Science and Technology Studies
2023
Friedrich Miescher Institute
2023
Korea University
2023
Gwangju Institute of Science and Technology
2023
Southwestern Medical Center
2023
Cryo-EM structure of human connexin 31.3 hemichannel reveals the selective permeability mechanism.
We examined six Arabidopsis thaliana genes from the DJ-1/PfpI superfamily for similarity to recently characterized bacterial and animal glyoxalases. Based on their sequence similarities, were classified into two sub-groups consisting of homologs human DJ-1 gene PH1704 Pyrococcus horikoshii. Unlike other species, all A. have tandem domains, which may been created by duplication. The AtDJ-1 proteins (a-f) expressed in Escherichia coli enzymatic assays with glyoxals. DJ-1d protein, belongs...
p62/SQSTM1 is the key autophagy adapter protein and hub of multi-cellular signaling. It was recently reported that N-end rule pathways are linked via p62. However, exact recognition mode degrading substrates regulation p62 in autophagic pathway remain unknown. Here, we present complex structures between ZZ-domain various type-1 type-2 N-degrons. The binding employed interaction with N-degrons differs from by classic N-recognins. also determined oligomerization PB1 domain can control...
Escherichia coli AcrAB-TolC is a multidrug efflux pump that expels wide range of toxic substrates. The dynamic nature the binding or low affinity between components has impeded elucidation how three assemble in functional state. Here, we created fusion proteins composed AcrB, transmembrane linker, and two copies AcrA. protein exhibited acridine pumping activity, suggesting reflects structure vivo. To discern assembling mode with TolC, AcrBA was incubated TolC chimeric containing aperture tip...
Edible plants have been widely used in traditional therapeutics because of the biological activities their natural ingredients, including anticancer, antioxidant, and anti-inflammatory properties. Plant sap contains such medicinal substances secondary metabolites provide unique chemical structures that contribute to therapeutic efficacy. extracts are known contain a variety extracellular vesicles (EVs) but effects EVs on various cancers not investigated. Here, we extracted from four...
Abstract Defects in plasma membrane repair can lead to muscle and heart diseases humans. Tripartite motif-containing protein (TRIM)72 (mitsugumin 53; MG53) has been determined rapidly nucleate vesicles at the site of damage, but underlying molecular mechanisms remain poorly understood. Here we present structure Mus musculus TRIM72, a complete model TRIM E3 ubiquitin ligase. We demonstrated that interaction between TRIM72 phosphatidylserine-enriched membranes is necessary for its oligomeric...
In contrast to most enveloped viruses, poxviruses produce infectious particles that do not acquire their internal lipid membrane by budding through cellular compartments. Instead, poxvirus immature are generated from atypical crescent-shaped precursors whose architecture and composition remain contentious. Here we describe the 2.6 Å crystal structure of vaccinia virus D13, a key structural component outer scaffold viral crescents. D13 folds into two jellyrolls decorated head domain novel...
Abstract Great effort has been devoted to discovering the basis of A3G-Vif interaction, key event HIV’s counteraction mechanism evade antiviral innate immune response. Here we show reconstitution complex and subsequent A3G ubiquitination in vitro report cryo-EM structure at 2.8 Å resolution using solubility-enhanced variants Vif. We present an atomic model interface, which assembles via known amino acid determinants. This assembly is not achieved by protein-protein interaction alone, but...
Fluorescent nanodiamonds (FNDs) are very promising fluorophores for use in biosystems due to their high biocompatibility and photostability. To overcome tendency aggregate physiological solutions, which severely limits the biological applications of FNDs, we developed a new non-covalent coating method using block copolymer, PEG-b-P(DMAEMA-co-BMA), or proteins such as BSA HSA. By simple mixing copolymer with cationic DMAEMA hydrophobic BMA moieties can strongly interact anionic on FND...
Abstract Podophages that infect gram-negative bacteria, such as Pectobacterium pathogen ΦM1, encode tail assemblies too short to extend across the complex cell wall. To overcome this, podophages a large protein (ejectosome) packaged inside viral capsid and correspondingly ejected during infection form transient channel spans periplasmic space. Here, we describe ejectosome of bacteriophage ΦM1 resolution 3.32 Å by single-particle cryo-electron microscopy (cryo-EM). The core consists...
The COVID-19 pandemic has intensified economic hardships, with potential negative impacts on food insecurity and infant feeding beliefs practices. relationship between practices during the is not yet fully understood. Neither how these relationships changed over course of waves pandemic. We examined in a cohort infants born various New York City (NYC). conducted cross-sectional analysis enrolled from birth into Mother-Baby Outcomes (COMBO) study March 2020 to May 2024. measured prior 30 days...
Bacillus cereus, a Gram-positive aerobic bacterium commonly found in soil, food, and water, forms endospores that can withstand harsh environmental conditions. The are encased protective spore coat consisting of multiple layers proteins, among which, CotE serves as crucial morphogenetic protein within the outer coat. In this study, we observed homotrimeric underwent further oligomerization induced by Ca2+ was subsequently dissociated dipicolinic acid, compound released from core during...
Natural medicinal plants have attracted considerable research attention for their potential as effective drugs. The roots, leaves and stems of the plant, Dendropanax morbifera, which is endemic to southern regions Asia, long been used a folk medicine treat variety diseases. However, sap this plant has not widely studied its bioactive properties yet be clearly elucidated. Here, we isolated extracellular vesicles from D. morbifera with goal improving intracellular delivery efficiency clinical...
Actin bundles are key factors in the mechanical support and dynamic reorganization of cytoskeleton. High concentrations multivalent counterions promote bundle formation through electrostatic attraction between actin filaments that negatively charged polyelectrolytes. In this study, we evaluate how physiologically relevant divalent cations affect mechanical, dynamic, structural properties bundles. Using a combination total internal reflection fluorescence microscopy, transmission electron...
Much attention has been focused on the design and synthesis of potent, cationic antimicrobial peptides (AMPs) that possess both anti-inflammatory activities. However, their development into therapeutic agents limited mainly due to large size (12 50 residues in length) poor protease stability.In an attempt overcome issues described above, a set ultra-short, His-derived (HDAMPs) developed for first time. Through systematic tuning pendant hydrophobic alkyl tails at N(π)- N(τ)-positions His,...