A5042331909- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Bacterial Genetics and Biotechnology
- Advanced Glycation End Products research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Sperm and Testicular Function
- Protein Structure and Dynamics
- Concrete and Cement Materials Research
- Biochemical and Structural Characterization
- Nutrition, Genetics, and Disease
- Genetic and phenotypic traits in livestock
- Microbial Metabolic Engineering and Bioproduction
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Innovative concrete reinforcement materials
- Genomics and Phylogenetic Studies
- Renal and related cancers
Konkuk University
2016-2025
Seoul National University
1996-2025
Electronics and Telecommunications Research Institute
2005-2024
Konkuk University Medical Center
2005-2024
The University of Texas at Austin
2023-2024
Korea Advanced Institute of Science and Technology
2013-2023
Samsung (South Korea)
2006-2023
Korea University
2017-2023
Wesleyan University
2021
Ajou University
2005-2020
For 10,000 years pigs and humans have shared a close complex relationship. From domestication to modern breeding practices, shaped the genomes of domestic pigs. Here we present assembly analysis genome sequence female Duroc pig (Sus scrofa) comparison with wild from Europe Asia. Wild emerged in South East Asia subsequently spread across Eurasia. Our results reveal deep phylogenetic split between European Asian boars ∼1 million ago, selective sweep indicates selection on genes involved RNA...
Jung-Hyun Lee, Jong Bhak and their colleagues report the whole-genome sequencing de novo assembly of a male minke whale genome, as well genome sequences three additional whales, fin whale, bottlenose dolphin finless porpoise. Their comparative analysis across cetaceans provides insights into adaptation to an aquatic lifestyle. The shift from terrestrial life by whales was substantial evolutionary event. Here we Our genomic identified expansion in lineage gene families associated with...
Human DJ-1 is a genetic cause of early-onset Parkinson's disease (PD), although its biochemical function unknown. We report here that human and homologs the mouse Caenorhabditis elegans are novel types glyoxalase, converting glyoxal or methylglyoxal to glycolic lactic acid, respectively, in absence glutathione. Purified proteins exhibit typical Michaelis-Menten kinetics, which were abolished completely mutants essential catalytic residues, consisting cysteine glutamic acid. The presence...
The overuse of antibiotics plays a major role in the emergence and spread multidrug-resistant bacteria. A molecularly targeted, specific treatment method for bacterial pathogens can prevent this problem by reducing selective pressure during microbial growth. Herein, we introduce nonviral strategy delivering genome editing material targeting antibacterial resistance. We apply CRISPR-Cas9 system, which has been recognized as an innovative tool highly efficient engineering different organisms,...
Human DJ-1 and Escherichia coli Hsp31 belong to ThiJ/PfpI family, whose members contain a conserved domain. is associated with autosomal recessive early onset parkinsonism molecular chaperone. Structural comparisons between DJ-1, Hsp31, an Archaea protease, member of lead the identification chaperone activity proteolytic Hsp31. Moreover, provide insights into how functional diversity realized in proteins that share evolutionarily On basis possible role pathogenesis Parkinson's disease...
The relationship between polymorphism of the bovine lymphocyte Ag (BoLA)-DRB3 gene and resistance susceptibility to persistent lymphocytosis (PL) caused by leukemia virus (BLV) was investigated. Exon 2 BoLA-DRB3 cloned from animals with BoLA haplotypes previously found be associated PL. Sequence analysis revealed presence amino acids Glu-Arg (ER) at putative binding residues 70 71 only in This correlation confirmed a case control study (n = 26) using an allele-specific polymerase chain...
Hsp31 encoded by hchA is known as a heat-inducible molecular chaperone. Although structure studies revealed that has putative catalytic triad consisting of Asp-214, His-186 and Cys-185, its enzymatic function, besides weak amino-peptidase activity, still unknown. We found displays glyoxalase activity catalyses the conversion methylglyoxal (MG) to d-lactate without an additional cofactor. The was completely abolished in hchA-deficient strain, confirming relationship between gene vivo....
Significance Pigs have many features that make them attractive as biomedical models, especially in regenerative medicine. Here, we introduced inactivating mutations simultaneously into both alleles of the recombination activating gene ( RAG ) 2 fibroblasts derived from minipigs and then used somatic-cell nuclear transfer to produce RAG2 −/− cloned animals with a severe immune deficiency (SCID) phenotype lacking T B cells. When human induced pluripotent (iPS) cells were injected these SCID...
Graphene, a two-dimensional carbon sheet with single-atom thickness, shows immense promise in several nanoscientific and nanotechnological applications, including sensors, catalysis, biomedicine. Although studies have shown the cytotoxicity of graphene oxide different cell types, there are no comprehensive on human embryonic kidney (HEK293) cells that include transcriptomic analysis an vitro investigation into mechanisms following exposure to oxide. Therefore, we exposed HEK293...
Solid tumors are frequently associated with resistance to chemotherapy because the fraction of hypoxic tumor cells is substantial. To understand underlying mechanism hypoxia on silver nanoparticle (AgNPs)-induced apoptosis, expression hypoxia-inducible factor (HIF)-1α, a hallmark hypoxia, was measured in presence and absence AgNPs. The results showed that HIF-1α upregulated after AgNPs treatment under both normoxic conditions. Cell viability assays promoted cell death cancer but not...
Summary The starvation‐induced csgD gene of Salmonella typhimurium encodes for the positive transcriptional regulator extracellular matrix components curli fimbriae and cellulose. To analyse regulatory elements promoter ( PcsgD ) response genetic studies combined with in vitro experiments were performed. Six binding sites (D1 to D6) OmpR, a regulator, identified by gel shifts DNase I footprints. While ompR is required expression, OmpR‐P D2 centred immediately upstream D1 at position −70.5...
After the knock-out (KO) of α1,3 galactosyltransfease (Gal-T), Hanganutziu-Deicher antigen became a major "non-Gal antigen" that is implicated in subsequent xenograft rejection. For deletion non-Gal antigen, we successfully produced zinc finger nuclease (ZFN)-mediated monoallelic/biallelic male and female CMP-N-acetylneuraminic acid hydroxylase (CMAH) KO miniature pigs: efficiency gene targeting (41.7%) was higher when donor DNA used with ZFN than those alone (9.1%). Monoallelic pigs had no...
AbstractAbstractSilver nanoparticles (AgNPs) are widely used as an antibiotic agent in textiles, wound dressings, medical devices, and appliances such refrigerators washing machines. The increasing use of AgNPs has raised concerns about their potential risks to human health. Therefore, this study was aimed determine the impact germ cell specific complications mice. administration results toxicity mice; however, a more detailed understanding effects on cells remains poorly understood. Here,...
Abstract Background Insects and animals can recognize surrounding environments by detecting thousands of chemical odorants. Olfaction is a complicated process that begins in the olfactory epithelium with specific binding volatile odorant molecules to dedicated receptors (ORs). OR proteins are encoded largest gene superfamily mammalian genome. Results We report here whole genome analysis receptor genes S. scrofa using conserved motifs known protein sequences from diverse species. identified...
Pigs (Sus scrofa) provide relevant biomedical models to dissect complex diseases due their anatomical, genetic, and physiological similarities with humans. Aberrant DNA methylation has been linked many of these is associated gene expression; however, the functional differences between porcine human patterns are largely unknown. RNA was isolated from eight tissue samples (fat, heart, kidney, liver, lung, lymph node, muscle, spleen) adult female Duroc utilized for pig genome sequencing...
The rapid development of nanotechnology has led to the use silver nanoparticles (AgNPs) in biomedical applications, including antibacterial, antiviral, anti-inflammatory, and anticancer therapies. molecular mechanism AgNPs-induced cytotoxicity not been studied thoroughly using a combination cellular assays RNA sequencing (RNA-Seq) analysis. In this study, we prepared AgNPs myricetin, an anti-oxidant polyphenol, their effects on NIH3T3 mouse embryonic fibroblasts as vitro model system explore...