Glioblastoma is the most common brain tumor. Median survival in unselected patients <10 months. The tumor harbors stem-like cells that self-renew and propagate upon serial transplantation mice, although clinical relevance of these has not been well documented. We have performed first genome-wide analysis directly relates gene expression profile nine enriched populations glioblastoma stem (GSCs) to five identically isolated cultivated from normal adult human brain. Although two cell types...

The nuclear pore complex is a transport channel embedded in the envelope and made up of 30 different components termed nucleoporins (Nups). In addition to their classical role transport, subset Nups has conserved regulation transcription via direct binding chromatin. molecular details this function remain obscure, it unknown how metazoan are recruited chromatin locations or what steps they regulate. Here, we demonstrate genome-wide physical association between Nup98 histone-modifying...

Sonic hedgehog (Shh) signaling patterns the vertebrate spinal cord by activating a group of transcriptional repressors in distinct neural progenitors somatic motor neuron and interneuron subtypes. To identify action this network, we performed genome-wide analysis regulatory actions three key ventral determinants mammalian tube patterning: Nkx2.2, Nkx6.1 Olig2. Previous studies have demonstrated that each factor acts predominantly as repressor, at least part, to inhibit alternative progenitor...

Cockayne syndrome is an inherited premature aging disease associated with numerous developmental and neurological defects, mutations in the gene encoding CSB protein account for majority of cases. Accumulating evidence suggests that functions transcription regulation, addition to its roles DNA repair, those defects this transcriptional activity might contribute clinical features syndrome. Transcription profiling studies have so far uncovered CSB-dependent effects on expression; however,...

Chalcone is a polyphenolic compound found abundantly in natural plant components. They have been acclaimed as potential antitumor compounds multiple tumor cells. However, not much attention has paid to elucidate its mechanism of action. Here, chalcone was demonstrated trigger endoplasmic reticulum (ER) stress-induced apoptosis through sulfonation IRE1α by ER-localized NADPH oxidase 4 (NOX4). IRE1α-sulfonation at cysteine residue shown induce "regulated IRE1α-dependent decay" (RIDD) mRNA...

Poly(lactide) (PLA) has received tremendous attention recently from researchers and industrialists due to its ability solve environmental problems related plastic pollution. However, PLA's brittleness, poor thermal stability, low elongation at break, melt processing prevent use in a broader spectrum of applications. Herein, we produced very tough thermally more stable PLA stereocomplex by simply mixing with organoalkoxysilane. The PLA/silane (sc-PLA-silane) composite was prepared simple...

To obtain fully degradable and super-tough poly(lactide-co-glycolide) (PLGA) blends, biodegradable star-shaped PCL-b-PDLA plasticizers were synthesized using natural originated xylitol as initiator. These blended with PLGA to prepare transparent thin films. Effects of added on mechanical, morphological, thermodynamic properties PLGA/star-shaped blends investigated. The stereocomplexation strong cross-linked network between PLLA segment PDLA effectively enhanced interfacial adhesion matrix....

Motivated by the widespread proliferation of wireless networks employing directional antennas, we study some capacitated covering problems arising in these networks. Geometrically, area covered a antenna with parameters α,ρ,r is set points polar coordinates (r,θ) such that r ≤ and α θ + ρ. Given customers, their positions on plane bandwidth demands, problem considered here to cover all customers minimum number antennas demands assigned an stays within bound. We consider two settings this In...

Polyethersulfone (PES) has outstanding thermal and dimensional stability. It is considered an engineering thermoplastic. However, its high coefficient of expansion (CTE) hinders use in automobiles, microelectronics, flexible display areas. To overcome (CTE), recent studies have focused on reducing CTE improving mechanical properties by adding nano-sized fillers or materials. The addition nanofiller nanofibrils to the PES matrix often a positive effect properties, making it substrate. obtain...

We investigate the cycles in transcription network of Saccharomyces cerevisiae. Unlike a similar Escherichia coli, it contains many cycles. characterize properties these and their place regulatory mechanism cell. Almost all cerevisiae are contained single strongly connected component, which we call LSCC (L for "largest"), except cycle two factors. The fact that includes almost is well explained by random graph with same in- out-degrees nodes. Among different physiological conditions, cell...

The topology of beta-sheets is defined by the pattern hydrogen-bonded strand pairing. Therefore, predicting hydrogen bonded partners a fundamental step towards beta-sheet topology. At same time, finding correct very difficult due to long range interactions involved in Additionally, patterns aminoacids involved, formations are general and therefore use for computational recognition specific contacts between strands. In this work, we report new pairing algorithm. To address above mentioned...

We study local search algorithms for networks with heterogeneous edge weights, testing them on scale-free and Erd\"os-R\'enyi networks. assume that the location of destination node is discovered when it two edges away, cost additive. It was previously shown a strategy preferring high-degree nodes reduces average over simple random walk. In prior work, case costs are randomly distributed, different preference investigated [high betweenness centrality (LBC)], found to be superior in networks,...

Abstract Background It has been proposed that secondary structure information can be used to classify (to some extend) protein folds. Since this method utilizes very limited about the structure, it is not surprising a higher error rate than approaches use full 3D fold description. On other hand, comparing of structures computing intensive. This raises question what extend decreased with each new source information, especially if still simple alignment algorithms. We consider whether closed...

This paper presents a power management system of the dye-sensitized solar cell (DSSC) for ubiquitous sensor network (USN) applications. The charge pump with luminance-controlled oscillator regulates load impedance DSSC to track maximum point (MPP) under various light intensities. low drop-out regulator hysteresis comparator supplies intermittent pulses that are wide enough USN communicate host transponder even dim conditions. With MPP tracking, approximately 50% more is harvested over range...

We investigated the question if CSB (Cockayne Syndrome complementation B) protein actively regulates gene transcription and how mutations in affect that regulatory role. Here we describe processed interpreted ChIP-seq data (deposited Gene Expression Omnibus with accession number GSE50171) obtained during an investigation of question, this analysis assisted generation hypothesis were subsequently validated using other types experiment.

이 연구의 목적은 교육대학교 및 사범대학에서 진로교육과 관련하여 운영 중인 강좌에서 다루고 있는 내용을 분석하여 교원 양성기관에서 진로교육 관련 교사교육에 시사점을 도출하는 데 있다. 주된 내용은 ① 초·중등 초․중등교원 양성기관의 교육과정 현황 분석, ② 교원양성기관에서의 내용 영역 도출, ③ 강좌의 ④ 대한 시사점 도출로, 문헌 분석 교원양성기관의 강좌 강의계획서 분석을 통하여 도출하였다. 결과, 첫째, 초등학교에서의 진로교육개념, 초등학교의 진로교육에서 교사의 역할 등의 실제로 모의수업을 통해 경험해 보는 강의가 필요하다. 또한, 초등 단계의 목표 중 중요한 부분의 하나가 긍정적인 직업관 형성이라는 점을 고려하여 그에 내용도 중요하게 다루어질 필요가 둘째, 중등교원 양성기관에서는 교직이수 대학원 과정보다 체계적으로 운영된다고 볼 수 사범대학 내에서도 교원의 진로교육역량을 개발하기 위한 교육 프로그램에 인식과 실천이 미흡하다고 예비교사의 강화하기 위해서는 예비교사가 필수로...

이 연구는 중학교 및 고등학교의「진로와 직업」교과서를 교과서별, 학교급별 비교 분석을 통해「진로와 직업」교과서 간 차별성과 함께 연계성 차이점을 파악하기 위한 목적으로 수행되었다. 이를 위해 2015 개정「진로와 4종, 고등학교 총 8종을 대상으로 교과서의 구성 체제와 내용 체제를 중심으로 실시하였다. 분석 결과 개별 체제에 대한 종합적인 모습과 차이를 확인할 수 있었으며 통해 다음과 같은 연구 결과를 도출하였다. 첫째, 「진로와 직업」교과서는 대영역별 비율에 있어 뚜렷한 보이는 것으로 나타났으며, 이는「진로와 직업」교과목표를 잘 반영한 결과라 볼 있다. 둘째, 고등학교「진로와 직업」교과서는「진로와 직업」교과 교육과정의 핵심개념과 내용요소를 전반적으로 충실히 반영하고 있으나, 교육과정 상에 제시된 내용요소가 모호하고 학교급 차이가 두드러지지 않는 경우에는 교과서별 상이한 학습내용, 학교급간 중복된 학습내용이 나타나는 것을 있었다. 셋째, 상이하여도 다루고 있는 학습 요소가...

Abstract We assembled a panel of 28 tissue pairs human and mouse with RNA-Seq data on gene expression. focused genes no 1-to-1 homology, because they pose special challenges. In this way, we identified expression patterns that identify explain differences between the two species suggest target for therapeutic applications. Here mention three examples. One pattern is observed by defining aggregate immunoglobulin (which have homology) as measure different levels an immune response. Lung, used...

Motivation: Differentiation of cells into different cell types involves many chromatin modifications, and mapping these modifications is a key computational task as researchers uncover aspects that process. Modifications associated with heterochromatin formation pose new challenges in this context because we must define very broad regions have only moderately stronger signal than the rest chromatin. Lamin-associated domains (LADs) are prime example such regions. Results: We present...