A5059889964- HIV Research and Treatment
- Analytical chemistry methods development
- Electrochemical Analysis and Applications
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Peripheral Artery Disease Management
- Enzyme Structure and Function
- Aortic Disease and Treatment Approaches
- Aortic aneurysm repair treatments
- Inorganic and Organometallic Chemistry
- Analytical Chemistry and Sensors
- Cardiac Valve Diseases and Treatments
- Ubiquitin and proteasome pathways
- Venous Thromboembolism Diagnosis and Management
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Atrial Fibrillation Management and Outcomes
- Advanced MRI Techniques and Applications
- Nuclear Structure and Function
- Advanced Battery Technologies Research
- Cerebrovascular and Carotid Artery Diseases
- Diagnosis and Treatment of Venous Diseases
- Mass Spectrometry Techniques and Applications
Frederick National Laboratory for Cancer Research
2016-2024
The Graduate University for Advanced Studies, SOKENDAI
2022
National Astronomical Observatory of Japan
2022
Mie University
2009-2022
Leidos (United States)
2016-2021
Leidos Biomedical Research Inc. (United States)
2016-2021
Kobe Kaisei Hospital
2019-2021
Government of the United States of America
2019
Nagasaki Kawatana Medical Center
2017
Fujita Health University Hospital
2016
Mutations in WNK kinases cause the human hypertensive disease pseudohypoaldosteronism type II (PHAII), but regulatory mechanisms of are not well understood. kelch-like 3 (KLHL3) and Cullin3 were also recently identified as causing PHAII. Therefore, new insights into hypertension can be gained by determining how these components interact they involved pathogenesis Here, we found that KLHL3 interacted with WNK4, induced WNK4 ubiquitination, reduced protein level. The interaction PHAII-causing...
Abstract We present two bright galaxy candidates at z ∼ 12–13 identified in our H -dropout Lyman break selection with 2.3 deg 2 near-infrared deep imaging data. These candidates, selected after careful screening of foreground interlopers, have spectral energy distributions showing a sharp discontinuity around 1.7 μ m, flat continuum 2–5 and nondetections <1.2 m the available photometric data sets, all which are consistent > 12 galaxy. An ALMA program targeting one shows tentative 4 σ...
Nucleic acid editing enzymes are essential components of the immune system that lethally mutate viral pathogens and somatically immunoglobulins, contribute to diversification lethality cancers. Among these seven human APOBEC3 deoxycytidine deaminases, each with unique target sequence specificity subcellular localization. While enzymology biological consequences have been extensively studied, mechanism by which APOBEC3s recognize edit DNA remains elusive. Here we present crystal structure a...
Abstract We present the number densities and physical properties of bright galaxies spectroscopically confirmed at z ∼ 7–14. Our sample is composed 60 spec 7–14, including recently = 12.34–14.18 with JWST, as well new confirmations 6.583–7.643 −24 < M UV −21 mag using ALMA Keck. JWST/NIRSpec observations have also revealed that very galaxy candidates 10–13 identified from ground-based telescope images before JWST are passive 3–4, emphasizing necessity strict screening spectroscopy in...
Recent studies have demonstrated the protective effects of supplementing free oxygen radical scavenging enzymes against hyperglycemia-induced embryonic malformations. In this study, glutathione (GSH)-dependent protection system in embryopathy was investigated. Rat embryos at early head-fold stage (day 9.5) cultured 66.7 mmol/l glucose for 48 h showed significant growth retardation and an increase frequency The concentration GSH activity rate-limiting GSH-synthesizing enzyme,...
The DEK proto-oncogene has been associated with human carcinogenesis-either as a fusion the CAN nucleoporin protein or when transcriptionally upregulated. Mechanisms of intracellular functions, however, have remained relatively unexplored. We recently demonstrated that expression is induced by high-risk papillomavirus (HPV) E7 in manner which dependent upon retinoblastoma function and implicated inhibition cellular senescence. Additionally, overexpression resulted significant life span...
Abstract The human APOBEC3G protein is a cytidine deaminase that generates to deoxy-uridine mutations in single-stranded DNA (ssDNA), and capable of restricting replication HIV-1 by generating viral genome. mechanism which specifically deaminates 5′-CC motifs has remained elusive since structural studies have been hampered due apparently weak ssDNA binding the catalytic domain APOBEC3G. We overcame problem highly active variant with higher affinity. Here, we present crystal structure this...
The 4E binding proteins (4E-BP1 and 4E-BP2) inhibit translation by to the limiting, proto-oncogenic initiation factor eIF4E. 4E-BPs produced in Escherichia coli had little or no folded structure, measured NMR CD. However, these inhibited reticulocyte lysate. Furthermore, they bound isolated mouse eIF4E, showing a few broader, dispersed new signals but general increase chemical shift dispersion. A peptide with sequence of 4E-BP1 residues 49−68 was sufficient bind eIF4E These results suggest...
Background Infiltration by perforin-secreting killer lymphocytes, such as T cells and natural cells, has been shown to be involved in the pathogenesis of vascular cell damage Takayasu’s arteritis. Methods Results To investigate immunological mechanisms involved, especially nature T-cell infiltration arteritis well atherosclerosis, we analyzed expression receptor (TCR) Vα Vβ genes infiltrating aortic tissue patients with atherosclerotic aneurysm polymerase chain reaction (PCR). We also...
Abstract The APOBEC3 (A3) family of human cytidine deaminases is renowned for providing a first line defense against many exogenous and endogenous retroviruses. However, the ability these proteins to deaminate deoxycytidines in ssDNA makes A3s double-edged sword. When overexpressed, can mutate genomic DNA resulting variety cancers. Although sequence context mutating varies among A3s, mechanism substrate specificity not well understood. To characterize A3A, systematic approach was used...
Abstract Great effort has been devoted to discovering the basis of A3G-Vif interaction, key event HIV’s counteraction mechanism evade antiviral innate immune response. Here we show reconstitution complex and subsequent A3G ubiquitination in vitro report cryo-EM structure at 2.8 Å resolution using solubility-enhanced variants Vif. We present an atomic model interface, which assembles via known amino acid determinants. This assembly is not achieved by protein-protein interaction alone, but...
Abstract APOBEC3 (or A3) enzymes have emerged as potential therapeutic targets due to their role in introducing heterogeneity viruses and cancer, often leading drug resistance. Inhibiting these has remained elusive initial phosphodiester (PO)-linked DNA-based inhibitors lack cellular stability potency. We enhanced both potency nuclease of 2′-deoxyzebularine (dZ) substrate-based oligonucleotide targeting two critical A3s: A3A A3G. While replacing the phosphate backbone with phosphorothioate...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTIdentification by NMR Spectroscopy of Residues at Contact Surfaces in Large, Slowly Exchanging Macromolecular ComplexesHiroshi Matsuo, Kylie J. Walters, Kenta Teruya, Takeyuki Tanaka, George T. Gassner, Stephen Lippard, Yoshimasa Kyogoku, and Gerhard WagnerView Author Information Department Biological Chemistry Molecular Pharmacology, Harvard Medical School 240 Longwood Avenue, Boston, Massachusetts 02115 Committee on Higher Degrees...