A5038212876- Enzyme Structure and Function
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Hemoglobin structure and function
- Advanced X-ray Imaging Techniques
- biodegradable polymer synthesis and properties
- Muscle Physiology and Disorders
- Proteins in Food Systems
- Microplastics and Plastic Pollution
- Heat shock proteins research
- Polymer crystallization and properties
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- X-ray Spectroscopy and Fluorescence Analysis
- Medical Imaging Techniques and Applications
- Porphyrin Metabolism and Disorders
- Force Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- X-ray Diffraction in Crystallography
- Microtubule and mitosis dynamics
Gifu University
2008-2024
SPring-8
2002-2019
RIKEN
1999-2008
University of Oxford
2008
Tokyo Institute of Technology
2006-2008
Shinshu University
2008
University of Warwick
2008
The University of Tokyo
2008
Osaka University
1989-2007
RIKEN Advanced Science Institute
2007
To investigate protein folding dynamics in terms of compactness, we developed a continuous-flow mixing device to make small-angle x-ray scattering measurements with the time resolution 160 micros and characterized radius gyration (R(g)) two intermediates cytochrome c (cyt c). The early intermediate possesses approximately 20 A R(g), which is smaller by 4 than that acid-unfolded state. R(g) later 18 A, close molten globule Considering alpha-helix content (f(H)) intermediates, clarified...
RIKEN beamline I (BL45XU) is an undulator with two branches. One for protein crystallography (PX) and the other small-angle x-ray scattering (SAXS). The beam split into branches by a diamond monochromator so that experiments can be done simultaneously [Yamamoto et al . (1995) Rev. Sci. Instrum. 66 , 1833-1835]. SAXS branch was designed studying weak interaction of proteins or subunits fibrous solutions especially using hydrostatic pressure. optics makes use good parallelism in order to...
The characterization of protein folding dynamics in terms secondary and tertiary structures is important elucidating the features intraprotein interactions that lead to specific folded structures. Apomyoglobin (apoMb), possessing seven helices termed A–E, G, H native state, has a intermediate composed A, helices, whose formation submillisecond time domain not been clearly characterized. In this study, we used rapid-mixing device combined with circular dichroism small-angle x-ray scattering...
The subunits of the dihydrolipoyl acetyltransferase (E2) component mammalian pyruvate dehydrogenase complex can form a 60-mer via association C-terminal I domain E2 at vertices dodecahedron. Exterior to this inner core structure, has (E1)-binding followed by two lipoyl domains, all connected mobile linker regions. assembled structure also includes (E3)-binding protein (E3BP) that binds its I′ domain. E3BP similarly regions connecting an E3-binding and composition E2·E3BP was thought be 60...
Abstract We constructed chimeric proteins that consist of two green fluorescent protein variants, EBFP and EGFP, connected by flexible linkers, (GGGGS) n ( = 3∼4), helical (EAAAK) 2∼5). The conformations the with various linkers were evaluated using small‐angle X‐ray scattering (SAXS). SAXS experiments showed introducing short 2∼3) causes multimerization, while longer 4∼5) solvate monomeric proteins. With moderate‐length 4), observed radius gyration R g ) maximum dimension D max 38.8 Å 120...
The annealing behavior of poly(l-lactic acid) (PLLA) and poly(d-lactic (PDLA) single crystals was investigated in order to reveal the mechanism chain diffusion their solid states, below melting points. structural changes sedimented single-crystal mats on continuous heating were followed real time by synchrotron small-angle X-ray scattering (SAXS) wide-angle diffraction (WAXD) measurements. crystal homopolymer showed a discontinuous thickening at an temperature each point. experimental...
In small-angle X-ray scattering experiments at high-brilliant synchrotron sources, protein aggregation results from radiation damage. The radiation-induced of lysozyme in solution was qualitatively evaluated based on forward and radii gyration. did not change below 400 Gy increased exponentially above this dose. is only seen beyond the critical dose rate, `dilution effect' known radiology also observed. Mass spectroscopy exposed to a monochromatic beam show any cleavage polypeptide chain....
Biodegradable fibers of poly[(R)-3-hydroxybutyrate-co-8%-(R)-3-hydroxyvalerate] (P(3HB-co-8%-3HV)) produced from wild-type bacteria were prepared by melt-spinning, followed in an amorphous state quenching near the glass transition temperature (Tg). High tensile strength over 1 GPa one-step drawing with small crystal nuclei grown isothermal crystallization Tg. This new technique is a very attractive method for obtaining strong low-molecular-weight polyesters bacteria. The highly ordered...
Deletion of 13 amino acids from the carboxyl terminus staphylococcal nuclease (WTSNase delta) results in a denatured, partially unfolded molecule that lacks significant persistent secondary structure but is relatively compact and monomeric under physiological conditions [Shortle & Meeker (1989) Biochemistry 28, 936-944; Flanagan et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 748-752]. Because these other properties SNase delta polypeptide, it useful model system for investigating...
Characterization of the conformational landscapes for proteins with different secondary structures is important in elucidating mechanism protein folding. The folding trajectory single-chain monellin composed a five-stranded β-sheet and helix was investigated by using pH-jump from alkaline unfolded to native state. kinetic changes overall size shape were measured circular dichroism spectroscopy small-angle x-ray scattering, respectively. formation tertiary structure monitored intrinsic...
Uniaxially oriented films with high tensile strength were processed from ultrahigh-molecular-weight poly[(R)-3-hydroxybutyrate] (P(3HB)) by a method combining hot-drawing near the melting point of polymer and two-step-drawing at room temperature. In two-step-drawn subsequently annealed film, P(3HB) molecular chains fall into two states: 2/1 helix (α-form) planar zigzag (β-form) conformations. The mechanism for generating β-form during was investigated time-resolved synchrotron wide-...
d-threo-1-Phenyl-2-decanoylamino-3-morpholino-1-propanol (d-PDMP) is a frequently used inhibitor of glycosphingolipid biosynthesis. However, some interesting characteristics d-PDMP cannot be explained by the inhibition glycolipid synthesis alone. In present study, we showed that inhibits activation lysosomal acid lipase late endosome/lysosome specific lipid, bis(monoacylglycero)phosphate (also called as lysobisphosphatidic acid), through alteration membrane structure lipid. When added to...
CCD detectors are now widely used in many synchrotron small-angle X-ray scattering beamlines. The use of an image intensifier with cooled (XR-II + CCD) was studied, especially for solution scattering. Two samples, polystyrene latex and apoferritin, were used. These two samples have fine structure the profile due to symmetry narrow size distribution. recorded profile, comparison that obtained by a position-sensitive proportional counter (PSPC), showed XR-II has much smaller practical dynamic...