A5058577911- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Nuclear Structure and Function
- Escherichia coli research studies
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Ubiquitin and proteasome pathways
- Invertebrate Immune Response Mechanisms
- Redox biology and oxidative stress
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- Trace Elements in Health
- Drug Transport and Resistance Mechanisms
- DNA Repair Mechanisms
- Aquaculture disease management and microbiota
- Diet, Metabolism, and Disease
- Folate and B Vitamins Research
- Cancer-related gene regulation
- Cancer Research and Treatments
- Genomics and Chromatin Dynamics
Seoul National University
2016-2025
Biocon (Switzerland)
2023
Can Tho University
2016-2021
Pusan National University
2006-2016
Busan Medical Center
2012-2013
Chung-Ang University
2012
International Drug Development
2010
Centre National de la Recherche Scientifique
2008
Institut de Chimie des Substances Naturelles
2008
University of Manchester
2008
Survivin, an apoptosis inhibitor/cell-cycle regulator, is critically required for suppression of and ensuring normal cell division in the G2/M phase cycle. It highly expressed a cycle-regulated manner localizes together with caspase-3 on microtubules within centrosomes. Whether survivin physiologically relevant caspase inhibitor has been unclear due to difficulties obtaining correctly folded finding right conditions inhibition assay. In this study, recombinant, active human was Escherichia...
The melanization reaction induced by activated phenoloxidase in arthropods must be tightly controlled because of excessive formation quinones and systemic damage to the hosts. However, molecular mechanism which phenoloxidase-induced melanin synthesis is regulated vivo largely unknown. It known that Spätzle-processing enzyme a key production cleaved Spätzle from pro-Spätzle Drosophila Toll pathway. Here, we provide biochemical evidence Tenebrio molitor converts both 79-kDa prophenoloxidase...
Wnt/beta-catenin signaling plays a central role in development and is also involved diverse array of diseases. Binding Wnts to the coreceptors Frizzled LRP6/5 leads phosphorylation PPPSPxS motifs intracellular region inhibition GSK3beta bound scaffold protein Axin. However, it remains unknown how specifically inhibited upon Wnt stimulation. Here, we show that overexpression LRP6 containing Ser/Thr rich cluster motif impairs activity cells. Synthetic peptides strongly inhibit vitro only when...
Crystal structure of RNA helicase domain from genotype 1b hepatitis C virus has been determined at 2.3 Å resolution by the multiple isomorphous replacement method. The consists three domains that form a Y-shaped molecule. One is NTPase containing two highly conserved NTP binding motifs. Another an motif. third helical contains no β-strand. molecule distinctively separated other forming interdomain cleft into which single stranded can be modeled. A channel found between pair symmetry-related...
The insect Toll signaling pathway is activated upon recognition of Gram-positive bacteria and fungi, resulting in the expression antimicrobial peptides via NF-kappaB-like transcription factor. This activation mediated by a serine protease cascade leading to processing Spätzle, which generates functional ligand receptor. Recently, we identified three proteases mediating induced lysine-type peptidoglycan bacteria. However, identities downstream components Gram-negative-binding protein 3...
The recognition of lysine-type peptidoglycans (PG) by the PG complex has been suggested to cause activation serine protease cascade leading processing Spätzle and subsequent Toll signaling pathway. So far, two proteases involved in pathway have identified. One is a modular functioning as an initial enzyme be recruited into complex. other Drosophila (SPE), terminal that converts pro-protein its processed form capable binding receptor. However, it remains unclear how signal transferred...
Recognition of lysine-type peptidoglycan by recognition protein (PGRP)-SA provokes the activation Toll and prophenoloxidase pathways. Here we reveal that a soluble fragment peptidoglycan, long glycan chain with short stem peptides, is potent activator Drosophila pathway cascade in beetle Tenebrio molitor. Using this fragment, present biochemical evidence clustering PGRP-SA molecules on required for cascade. We subsequently highlight lysozyme-mediated partial digestion highly cross-linked...
Significance In gram-negative bacteria, OxyR is the master peroxide sensor that regulates transcription of defense genes in response to a low level cellular H 2 O via rapid kinetic reaction. this study, we present first, our knowledge, full-length structures peroxide-sensing regulator together with an oxidation intermediate-mimicking structure. The show all structural features describing tetrameric assembly and bound molecule near conserved cysteine. Combining results, reveal step-by-step...
Hutchinson-Gilford progeria syndrome (HGPS) is a rare autosomal dominant genetic disease that caused by silent mutation of the LMNA gene encoding lamins A and C (lamin A/C). The G608G generates more accessible splicing donor site than does WT produces an alternatively spliced product called progerin, which also expressed in normal aged cells. In this study, we determined progerin binds directly to lamin A/C induces profound nuclear aberrations. Given observation, performed random screening...
Abstract Nuclear structure and function are governed by lamins, which intermediate filaments that mostly consist of α-helices. Different lamin assembly models have been proposed based on low resolution fragmented structures. However, their mechanisms still poorly understood at the molecular level. Here, we present crystal a long human fragment 3.2 Å allows visualization features full-length protein. The shows an anti-parallel arrangement two coiled-coil dimers, is important for process. We...
The methylation of histone H3 lysine 79 (H3K79) is an active chromatin marker and prominent in actively transcribed regions the genome; however, demethylase H3K79 remains unknown despite intensive research. Here, we show that KDM2B, also known as FBXL10 a member Jumonji C family proteins for its H3K36 activity, di- trimethyl demethylase. We demonstrate KDM2B induces transcriptional repression HOXA7 MEIS1 via occupancy promoters demethylation H3K79. Furthermore, genome-wide analysis suggests...
SARS-CoV-2, the coronavirus strain that initiated COVID-19 pandemic, and its subsequent variants present challenges to vaccine development treatment. As evades host innate immune response at initial stage of infection, disease can have a long nonsymptomatic period. The uridylate-specific endoribonuclease Nsp15 processes viral genome for replication cleaves polyU sequence in RNA interfere with system. This study screened natural compounds vitro identify inhibitors against from SARS-CoV-2....
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolism. Compared with many different amylases that are able to hydrolyze only α-d-(1,4)-glycosidic bonds, maltogenic exhibit catalytic versatility: α-d-(1,4)- α-d-(1,6)-glycosidic bonds transglycosylation oligosaccharides C3-, C4-, or C6-hydroxyl groups various acceptor mono- disaccharides. It has been speculated property enzymes is linked additional ∼130 residues at N terminus absent other typical...
Bacterial Delta5-3-ketosteroid isomerase (KSI) catalyzes a stereospecific isomerization of steroid substrates at an extremely fast rate, overcoming large disparity pKa values between catalytic residue and its target. The crystal structures KSI from Pseudomonas putida the enzyme in complex with equilenin, analogue reaction intermediate, have been determined 1.9 2.5 A resolution, respectively. reveal that side chains Tyr14 Asp99 (a newly identified residue) form hydrogen bonds directly...
Differentially from other kinds of Ras, oncogenic K-Ras, which is mutated approximately 30% human cancer, does not induce apoptosis and senescence. Here, we provide the evidence that K-Ras abrogates p53 function expression through induction Ataxia telangiectasia-mutated Rad3-related mediated Snail stabilization. directly binds to DNA binding domain diminishes tumor-suppressive p53. Thus, elimination si-RNA can in K-Ras-mutated cells, whereas mutant suppress regardless status. Chemicals,...
Escherichia coli AcrAB-TolC is a multidrug efflux pump that expels wide range of toxic substrates. The dynamic nature the binding or low affinity between components has impeded elucidation how three assemble in functional state. Here, we created fusion proteins composed AcrB, transmembrane linker, and two copies AcrA. protein exhibited acridine pumping activity, suggesting reflects structure vivo. To discern assembling mode with TolC, AcrBA was incubated TolC chimeric containing aperture tip...