A5027388422- Protein Hydrolysis and Bioactive Peptides
- Invertebrate Immune Response Mechanisms
- Antimicrobial Peptides and Activities
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
- Neurobiology and Insect Physiology Research
- Food Quality and Safety Studies
- Neuropeptides and Animal Physiology
- Aquaculture Nutrition and Growth
- Marine Sponges and Natural Products
- Nutrition, Health and Food Behavior
- Insect Utilization and Effects
- Echinoderm biology and ecology
- Agriculture, Soil, Plant Science
- Insect and Pesticide Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Insect Resistance and Genetics
- Insect behavior and control techniques
- Marine Bivalve and Aquaculture Studies
- Bioactive Compounds and Antitumor Agents
- Fish Ecology and Management Studies
- Insect and Arachnid Ecology and Behavior
- Zebrafish Biomedical Research Applications
- Physiological and biochemical adaptations
- Epigenetics and DNA Methylation
Korea University
2024
Pukyong National University
2005-2023
University of Seoul
2022
Pusan National University
2007-2019
Yonsei University College of Dentistry
2017
Yonsei University
2017
University of Massachusetts Chan Medical School
2009-2014
Soonchunhyang University
2008-2014
Seoul National University
1996
The melanization reaction induced by activated phenoloxidase in arthropods must be tightly controlled because of excessive formation quinones and systemic damage to the hosts. However, molecular mechanism which phenoloxidase-induced melanin synthesis is regulated vivo largely unknown. It known that Spätzle-processing enzyme a key production cleaved Spätzle from pro-Spätzle Drosophila Toll pathway. Here, we provide biochemical evidence Tenebrio molitor converts both 79-kDa prophenoloxidase...
The Drosophila NF-kappaB transcription factor Relish is an essential regulator of antimicrobial peptide gene induction after gram-negative bacterial infection. a bipartite precursor protein, with N-terminal Rel homology domain and C-terminal IkappaB-like domain, similar to mammalian p100 p105. Unlike these homologs, endoproteolytically cleaved infection, allowing the module translocate nucleus. Signal-dependent activation Relish, including cleavage, requires both IkappaB kinase (IKK)...
The insect Toll signaling pathway is activated upon recognition of Gram-positive bacteria and fungi, resulting in the expression antimicrobial peptides via NF-kappaB-like transcription factor. This activation mediated by a serine protease cascade leading to processing Spätzle, which generates functional ligand receptor. Recently, we identified three proteases mediating induced lysine-type peptidoglycan bacteria. However, identities downstream components Gram-negative-binding protein 3...
The recognition of lysine-type peptidoglycans (PG) by the PG complex has been suggested to cause activation serine protease cascade leading processing Spätzle and subsequent Toll signaling pathway. So far, two proteases involved in pathway have identified. One is a modular functioning as an initial enzyme be recruited into complex. other Drosophila (SPE), terminal that converts pro-protein its processed form capable binding receptor. However, it remains unclear how signal transferred...
Recognition of lysine-type peptidoglycan by recognition protein (PGRP)-SA provokes the activation Toll and prophenoloxidase pathways. Here we reveal that a soluble fragment peptidoglycan, long glycan chain with short stem peptides, is potent activator Drosophila pathway cascade in beetle Tenebrio molitor. Using this fragment, present biochemical evidence clustering PGRP-SA molecules on required for cascade. We subsequently highlight lysozyme-mediated partial digestion highly cross-linked...
Abstract Aims To investigate whether fatty liver per se is an independent risk factor of incident type 2 diabetes mellitus (T2DM) in Koreans. Methods We examined the clinical and laboratory data 5372 non‐diabetic participants (3670 men 1702 women; age 46.8 ± 8.4 years, range 20–79 years) who underwent voluntary medical check‐ups 2000 follow‐up examinations 2005. Results Two hundred thirty‐three (4.3%) developed T2DM after 5 years. Univariate analysis showed that development was associated...
Serpins are known to be necessary for the regulation of several serine protease cascades. However, mechanisms how serpins regulate innate immune responses invertebrates not well understood due uncertainty identity proteases targeted by serpins. We recently reported molecular activation three protease-mediated Toll and melanin synthesis cascades in a large beetle, Tenebrio molitor. Here, we purified novel (SPN40, SPN55, SPN48) from hemolymph T. These made specific serpin-serine pairs with...
Intensification of aquaculture has led to frequent occurrence disease outbreaks. To deal with this issue antibiotics are a widely-preferred control strategy, but one that poses risks the environment and humans, if used indiscriminately. In pursuit an alternative, probiotics have emerged recently among viable alternatives for health management in aquaculture. The prophylactic use farmed shellfish species, i.e., shrimp, prawn, crab, crayfish, oyster abalone, been demonstrated enhance...
The Riptortus-Burkholderia symbiotic system is an experimental model for studying the molecular mechanisms of insect-microbe gut symbiosis. When midgut Riptortus pedestris was investigated by light and transmission electron microscopy, lumens crypts that harbor colonizing Burkholderia symbionts were occupied extracellular matrix consisting polysaccharides. This observation prompted us to search symbiont genes involved in induction biofilm formation examine whether biofilms are necessary...
Many insects possess symbiotic bacteria that affect the biology of host. The level symbiont population in host is a pivotal factor modulates biological outcome association. Hence, should be maintained at proper by host's control mechanisms. Several mechanisms for controlling intracellular symbionts have been reported, while extracellular gut are poorly understood. bean bug Riptortus pedestris harbors betaproteobacterial genus Burkholderia midgut organ designated M4 region. We found M4B...
Calcitonin (CT) is a peptide hormone released by the thyroid gland that regulates blood Ca2+ levels in mammals. The CT gene alternatively spliced, with one transcript encoding and another CT-like neuropeptide calcitonin-gene related (α-CGRP), which powerful vasodilator. Other CT-related peptides vertebrates include adrenomedullin, amylin intermedin, also act as smooth muscle relaxants. evolutionary origin of CT-type has been traced to bilaterian common ancestor protostomes deuterostomes...
Recent studies suggest that vertebrate and invertebrate defensins have evolved from two independent ancestors, both could share origins with animal toxins. Here, we purified novel sea anemone neurotoxin (BDS)-like antimicrobial peptides (AMPs)-Crassicorin-I its putative homolog (Crassicorin-II)-from the pharynx extract of an anthozoan (Urticina crassicornis). Based on structural analyses cDNA cloning, mature Crassicorin-I represents a cationic AMP likely generated precursor comprising 40...
Abstract Neuropeptides that act as muscle relaxants have been identified in chordates and protostomian invertebrates but little is known about the molecular identity of neuropeptides deuterostomian (e.g. echinoderms) are ‘evolutionary intermediates’ protostomes. Here, we used apical starfish Patiria pectinifera to assay for myorelaxants extracts this species. A hexadecapeptide with amino acid sequence Phe‐Gly‐Lys‐Gly‐Gly‐Ala‐Tyr‐Asp‐Pro‐Leu‐Ser‐Ala‐Gly‐Phe‐Thr‐Asp was designated myorelaxant...
Controversies still exist regarding the relative contributions of insulin resistance and β-cell dysfunction to pathogenesis Type 2 diabetes in different populations. We examined associations baseline function indices with development Koreans.We analysed clinical laboratory data 17 878 Korean adults (age 20-79 years) who underwent routine medical examinations a median interval 3.5 years (range 2.5-4.7 years). Using homeostasis model assessment, (HOMA-IR) (HOMA-%B) at were assessed.Those...