A5037119938- SARS-CoV-2 and COVID-19 Research
- Bacillus and Francisella bacterial research
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
- Viral Infections and Outbreaks Research
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Animal Virus Infections Studies
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Vibrio bacteria research studies
- Escherichia coli research studies
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- COVID-19 Clinical Research Studies
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Probiotics and Fermented Foods
- Viral Infections and Immunology Research
- Tea Polyphenols and Effects
- Cytokine Signaling Pathways and Interactions
- vaccines and immunoinformatics approaches
- Clostridium difficile and Clostridium perfringens research
International Vaccine Institute
2012-2023
Korea Disease Control and Prevention Agency
2018-2021
Korea Center for Disease Control and Prevention
2018
Seoul National University
1993-2017
Catholic University of Korea
2007-2009
Seoul St. Mary's Hospital
2008
Probiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates they responsible functions livestock. The present study demonstrated lipoteichoic acid of Lactobacillus plantarum (Lp.LTA) confers anti-inflammatory responses porcine intestinal epithelial line, IPEC-J2. A synthetic analog viral...
SARS-CoV-2, the coronavirus strain that initiated COVID-19 pandemic, and its subsequent variants present challenges to vaccine development treatment. As evades host innate immune response at initial stage of infection, disease can have a long nonsymptomatic period. The uridylate-specific endoribonuclease Nsp15 processes viral genome for replication cleaves polyU sequence in RNA interfere with system. This study screened natural compounds vitro identify inhibitors against from SARS-CoV-2....
Abstract Background Numerous vaccine strategies are being advanced to control SARS-CoV-2, the cause of COVID-19 pandemic. EuCorVac-19 (ECV19) is a recombinant protein nanoparticle that displays SARS-CoV-2 receptor-binding domain (RBD) on immunogenic nanoliposomes. Methods Initial study phase 2 randomized, observer-blind, placebo-controlled trial assess immunogenicity, safety, and tolerance ECV19 was carried out between July October 2021. Two hundred twenty-nine participants were enrolled at...
<title>Abstract</title> Adjuvants play a crucial role in enhancing vaccine-induced immune responses by shaping the magnitude and quality of humoral cellular immunity. However, mechanism through which different adjuvants modulate effector functions is not fully understood. Here, we developed an International Vaccine Institute liposome-based adjuvant (ILA) comprehensively compared profiles mice following administration SARS-CoV-2 spike (S) protein formulated with either ILA or aluminum...
Although the 56-kDa protein of Rickettsia tsutsugamushi has been presumed to play important roles in generating protective immunity against scrub typhus, studies this have impeded. We used recombinant R. Boryong fused with maltose-binding Escherichia coli (MBP-Bor56) analyze its ability induce a C3H/HeDub murine model. Intraperitoneal immunization mice MBP-Bor56 resulted an increase 50% minimal lethal dose more than 160 times compared that for control mice. Splenic mononuclear cells from...
The 56-kDa protein of Rickettsia tsutsugamushi, which is located on the rickettsial surface, has been shown to be an immunodominant antigen. gene that encodes R. tsutsugamushi Boryong (bor56) was cloned. Sequencing revealed open reading frame 1,602 bp encoding 534 amino acids with a molecular weight 56,803. (Bor56) expressed as fusion maltose-binding Escherichia coli by deleting 252 from 5' end and subcloning it into StuI site pIH821. recombinant purified amylose column chromatography for...
Although chemotherapy remains among the best treatment options for most cancers, adjuvant therapies such as dendritic cell (DC)-based immunotherapy have been added to protocols destroy residual tumour cells. Combination with low-dose temozolomide (TMZ) followed by vaccination TAT-survivin-pulsed DCs enhanced T-cell responses specific survivin and improved survival rate, compared DC alone or TMZ alone. Moreover, antigen-specific immunity appears be mediated CD8(+) T cells, determined in vitro...
The genes encoding the 56-kDa polypeptides were amplified by polymerase chain reaction from genomic DNAs of three serotypes Rickettsia tsutsugamushi, Gilliam, Karp, and Boryong. products cloned into expression vector pIH821, recombinant antigens expressed in Escherichia coli as fusion proteins with maltose-binding protein. purified affinity chromatography for sensitization sheep erythrocytes. evaluated 89 serum specimens health blood donors, 94 scrub typhus patients, 31 patients other...
The development of safe and effective vaccines to prevent SARS-CoV-2 infections remains an urgent priority worldwide. We have used a recombinant vesicular stomatitis virus (rVSV)-based prime-boost immunization strategy develop COVID-19 vaccine candidate. constructed VSV genomes carrying exogenous genes resulting in the production avirulent rVSV full-length spike protein (SF), S1 subunit, or receptor-binding domain (RBD) plus envelope (E) SARS-CoV-2. Adding honeybee melittin signal peptide...
Abstract In human intestinal epithelial cells, IFN-γ induces BAFF expression through the signaling pathway of JAK/STAT and GAS, in promoter region gene. plays an important role development B cells. Here, we investigated effect on induced soluble membrane-bound production a dose- time-dependent manner. IFN-γ-induced release from polarized cells was observed apical basolateral compartments. JAK I inhibitor suppressed expression. Moreover, enhanced STAT1 phosphorylation IRF-1. Transient...
Although dendritic cells (DC) have been well demonstrated as a strong cellular adjuvant for tumor vaccine, there are several limitations clinical application. A protein‐based vaccine using potent is an appealing approach antigen‐specific immunotherapy because of their simplicity, safety, efficacy and capacity repeated administration. CpG‐oligodeoxynucleotides (ODN) used adjuvants to stimulate innate adaptive immune responses cancer treatment. The authors evaluated the effects CpG‐ODN in...
RNA electroporation as a gene delivery method is more feasible and safer compared with viral vectors. RNA-loaded dendritic cells (DC) have been used to induce T cell responses against tumor rejection antigens B can also act antigen-presenting for cellular vaccines. In this study, we DC, after carcinoembryonic antigen (CEA) RNA, their capacity generate cytotoxic lymphocytes antitumor immunity. Vaccination using these induced levels of IFN-gamma-secreting comparable those by DC. Intravenous...
Abstract P.1. or gamma variant also known as the Brazil variant, is one of variants concern (VOC) which appears to have high transmissibility and mortality. To explore potency CT-P59 monoclonal antibody against P.1 we tried conduct binding affinity, in vitro neutralization, vivo animal tests. In assays revealed that able neutralize spite reduction its affinity a RBD (receptor domain) mutant protein including K417T/E484K/N501Y neutralizing activity pseudoviruses live viruses. contrast, hACE2...
Growing concerns about unpredictable influenza pandemics require a broadly protective vaccine against diverse strains. One of the promising approaches was T cell‐based vaccine, but narrow breadth ‐cell immunity due to immunodominance hierarchy established by previous infection and efficacy only mild challenge condition are important hurdles overcome. To model in humans an experimental setting, influenza‐primed C 57 BL /6 mice were chosen boosted with mixture vaccinia recombinants,...
Abstract Background Bacillus ancthracis causes cutaneous, pulmonary, or gastrointestinal forms of anthrax . B. anthracis is a pathogenic bacterium that potentially to be used in bioterrorism because it can produced the form spores. Currently, protective antigen (PA)-based vaccines are being for prevention anthrax, but necessary develop more safe and effective due their prolonged immunization schedules adverse reactions. Methods We selected lipoprotein GBAA0190, potent inducer host immune...
As the number of large-scale studies involving multiple organizations producing data has steadily increased, an integrated system for a common interoperable format is needed. In response to coronavirus disease 2019 (COVID-19) pandemic, global efforts are underway develop vaccines and therapeutics. We therefore observing explosion in proliferation COVID-19 data, interoperability highly requested institutions participating simultaneously pandemic research.In this study, laboratory information...