A5058516691- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Animal Virus Infections Studies
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Vector-Borne Animal Diseases
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Virology and Viral Diseases
- Cervical Cancer and HPV Research
- Parvovirus B19 Infection Studies
- Antimicrobial Resistance in Staphylococcus
- RNA and protein synthesis mechanisms
- COVID-19 epidemiological studies
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Bacterial biofilms and quorum sensing
- Biochemical Analysis and Sensing Techniques
- COVID-19 diagnosis using AI
Andong National University
2023-2024
Korea Research Institute of Bioscience and Biotechnology
2014-2023
Seoul National University
2023
Korea University of Science and Technology
2016-2022
Konkuk University
2021
Korea University
2019
Wageningen University & Research
2019
Vietnam National University of Agriculture
2019
Sejong University
2019
St. Jude Children's Research Hospital
2011-2017
Avian Flu in Ferrets A recent outbreak of avian H7N9 influenza humans eastern China has been closely monitored for any evidence human-to-human transmission and its potential sparking a pandemic. Zhu et al. (p. 183 , published online 23 May) examined the behavior virus ferret, mammalian model human influenza. The was excreted by ferrets could be transmitted readily contact but displayed limited capacity airborne infectivity. pathology is similar to H1N1, it seems that factors other than...
African swine fever is one of the most dangerous diseases swine. We confirmed 2019 outbreak in Vietnam by real-time reverse transcription PCR. The causative strain belonged to p72 genotype II and was 100% identical with viruses isolated China (2018) Georgia (2007). International prevention control collaboration needed.
The endemicity of highly pathogenic avian influenza (HPAI) A(H5N1) viruses in Asia has led to the generation reassortant H5 strains with novel gene constellations. A newly emerged HPAI A(H5N8) virus caused poultry outbreaks Republic Korea 2014. Because emerging high-pathogenicity continue pose public health risks, it is imperative that their pathobiological properties be examined. Here, we characterized A/mallard duck/Korea/W452/2014 (MDk/W452(H5N8)), a representative virus, and evaluated...
Since severe acute respiratory syndrome (SARS) and Middle East (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, transmission, methods for a rapid accurate differential diagnosis are important. Therefore, the aim of this study was develop duplex real-time reverse transcription (RT)-PCR method simultaneous detection these viruses. Primers probes that target conserved spike S2 region human SARS-CoV, MERS-CoV, their related bat CoVs were...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed the detection of SARS-CoV-2, advanced sensing methods are required diagnosis, screening, and surveillance disease 2019 (COVID-19). Here, we report surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), magnetic beads SARS-CoV-2. The selected pair against SARS-CoV-2...
Influenza virus entry is mediated by the acidic-pH-induced activation of hemagglutinin (HA) protein. Here, we investigated how a decrease in HA pH (an increase acid stability) influences properties highly pathogenic H5N1 influenza mammalian hosts. We generated isogenic A/Vietnam/1203/2004 (H5N1) (VN1203) viruses containing either wild-type protein (activation 6.0) or an HA2-K58I point mutation (K to I at position 58) 5.5). The VN1203-HA2-K58I had replication kinetics similar those VN1203...
Neuraminidase inhibitors (NAIs) have been widely used to control influenza virus infection, but their increased use could promote the global emergence of resistant variants. Although various mutations associated with NAI resistance identified, amino acid substitutions that confer multidrug undiminished viral fitness remain poorly understood. We therefore screened a known mutation(s) currently approved NAIs oseltamivir, zanamivir, and peramivir by assessing recombinant viruses mutant...
Significance The endonuclease domain within the influenza virus heterotrimeric replication machinery is essential and represents an attractive drug target. It important to understand structural basis of potential inhibitor resistance, design appropriate inhibitors prioritize candidates that are unlikely cause rapid development clinically-relevant resistance mutations. Using a prototypical (L-742,001), we used mutagenesis select for competent resistant mutants studied functional observed...
Bat species around the world have recently been recognized as major reservoirs of several zoonotic viruses, such severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East (MERS-CoV), Nipah virus and Hendra virus. In this study, consensus primer-based reverse transcriptase polymerase chain reactions (RT-PCRs) high-throughput sequencing were performed to investigate viruses in bat faecal samples collected at 11 natural habitat sites from July December 2015 Korea. Diverse...
ABSTRACT A balance between the functions of influenza virus surface proteins hemagglutinin (HA) and neuraminidase (NA) is thought to be important for transmission viruses humans. Here we describe two pandemic H1N1 viruses, A/swine/Virginia/1814-1/2012 A/swine/Virginia/1814-2/2012 (pH1N1 low -1 -2, respectively), that were isolated from swine symptomatic influenza. The enzymatic activity NA these was almost undetectable, while HA binding affinity α2,6 sialic acids greater than highly...
Highly pathogenic avian influenza (HPAI) A(H5N6) and A(H5N8) virus infections resulted in the culling of more than 37 million poultry Republic Korea during 2016/17 winter season. Here we characterize two representative viruses, A/Environment/Korea/W541/2016 [Em/W541(H5N6)] A/Common Teal/Korea/W555/2017 [CT/W555(H5N8)], evaluate their zoonotic potential various animal models. Both Em/W541(H5N6) CT /W555(H5N8) are novel reassortants derived from gene pools wild bird viruses present migratory...
The mounting of a specific immune response against the human papillomavirus type 16 E7 protein (HPV16 E7) is important for eradication HPV16 E7-expressing cancer cells from cervical mucosa. To induce mucosal by oral delivery antigen, we expressed antigen on surface Lactobacillus casei employing novel display system in which poly-gamma-glutamic acid (gamma-PGA) synthetase complex A (PgsA) Bacillus subtilis (chungkookjang) was used as an anchoring motif. After expression confirmed Western...
Efficient worldwide swine surveillance for influenza A viruses is urgently needed; the emergence of a novel reassortant pandemic H1N1 (pH1N1) virus in 2009 demonstrated that can be direct source and potential prevalent populations must monitored. We used ferret model to assess pathogenicity transmissibility predominant Korean triple-reassortant (TRSw) H1N2 H3N2 genetically related North American strains. Although most TRSw were moderately pathogenic, one [A/Swine/Korea/1204/2009; Sw/1204...
The bat paramyxovirus B16-40 was first isolated in Korea this study. Using the virus, we could obtain not only genomic information, but also several biological characteristics of virus. In phylogenetic analysis, virus found to belong recently proposed genus Shaanvirus. Through sequence analyses and vitro testing, have haemagglutinin-neuraminidase (HN) protein as one structural proteins. When mouse antiserum generated against tested, it cross-reactive human parainfluenza 1 an indirect...
Bovine coronavirus (BCoV) is a causative agent of respiratory and enteric diseases in cattle calves. BCoV infection was also evident captive wild ruminants. Recently, water deer are recognized as the most common wildlife to approach farmhouses livestock barns Korea. Therefore, we investigated 77 nasal swab samples from non-captive (Hydropotes inermis) between November 2016 September 2017 identified three positive for coronavirus, indicating potential shedding. The full genomic sequences were...
Abstract Point-of-care tests (POCT) for pathogens are considered important low-resource countries and facilities. Although lateral flow immunoassays (LFIA) have many advantages including speed ease of use, their sensitivity is limited without specific equipment. Furthermore, response cannot be enhanced through enzymatic reactions. Owing to these limitations, LFIAs not yet been generally adopted as the standard protocol in vitro analysis infectious pathogens. We aimed develop a novel...
The ongoing coronavirus disease 2019 (COVID-19) pandemic and its overlap with the influenza season lead to concerns over severe caused by virus acute respiratory syndrome 2 (SARS-CoV-2) co-infections. Using a Syrian hamster co-infection model, SARS-CoV-2 A/California/04/2009 (H1N1), we found (a) more in co-infected animals compared those infected alone but not infection alone; (b) altered hematological changes only animals; (c) tropism tracts of animals. Overall, our study revealed that is...