A5100644148- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Fire effects on ecosystems
- Immune Cell Function and Interaction
- Labor market dynamics and wage inequality
- Immune Response and Inflammation
- Plant Virus Research Studies
- Retirement, Disability, and Employment
- Phytochemicals and Antioxidant Activities
- Food Quality and Safety Studies
- Traditional Chinese Medicine Studies
- Biosensors and Analytical Detection
- Healthcare and Venom Research
- Virology and Viral Diseases
Institute for Basic Science
2023-2024
Chungbuk National University
2014-2023
Korea Research Institute of Bioscience and Biotechnology
2011-2020
Seoul National University
2018-2020
Celltrion (South Korea)
2020
Korea Center for Disease Control and Prevention
2020
Hallym University
2020
Korea Disease Control and Prevention Agency
2020
National University College
2020
New Generation University College
2020
Background. Although Middle East Respiratory Syndrome coronavirus (MERS-CoV) is characterized by a risk of nosocomial transmission, the detailed mode transmission and period virus shedding from infected patients are poorly understood. The aims this study were to investigate potential role environmental contamination MERS-CoV in healthcare settings define viable MERS patients.
Abstract Background In addition to seasonal influenza viruses recently circulating in humans, avian (AIVs) of H5N1, H5N6 and H7N9 subtypes have also emerged demonstrated human infection abilities with high mortality rates. Although viral infections are usually diagnosed using isolation serological/molecular analyses, the cost, accessibility, availability these methods may limit their utility various settings. The objective this study was develop optimized a multiplex detection system for...
The endemicity of highly pathogenic avian influenza (HPAI) A(H5N1) viruses in Asia has led to the generation reassortant H5 strains with novel gene constellations. A newly emerged HPAI A(H5N8) virus caused poultry outbreaks Republic Korea 2014. Because emerging high-pathogenicity continue pose public health risks, it is imperative that their pathobiological properties be examined. Here, we characterized A/mallard duck/Korea/W452/2014 (MDk/W452(H5N8)), a representative virus, and evaluated...
Given the current coronavirus disease 2019 (COVID-19) pandemic, coinfection of severe acute respiratory syndrome 2 (SARS-CoV-2) and influenza A virus (IAV) is a major concern for public health. However, immunopathogenic events occurring with coinfections SARS-CoV-2 IAV remain unclear. Here, we report pathogenic immunological consequences H1N1 in K18-hACE2 transgenic mouse model. Compared single infection or IAV, not only prolonged primary period but also increased immune cell infiltration...
Cases of laboratory-confirmed SARS-CoV-2 reinfection have been reported in a number countries. Further, the level natural immunity induced by infection is not fully clear, nor it clear if primary protective against reinfection. To investigate potential association between serum antibody titres and SARS-CoV-2, ferrets with different levels NAb after were subjected to heterologous strain. All reinfected showed active virus replication upper respiratory gastro-intestinal tracts. However, high...
Abstract Few studies have used a longitudinal approach to describe the immune response SARS-CoV-2 infection. Here, we perform single-cell RNA sequencing of bronchoalveolar lavage fluid cells longitudinally obtained from SARS-CoV-2-infected ferrets. Landscape analysis lung microenvironment shows distinct changes in cell proportions and characteristics compared uninfected control, at 2 5 days post-infection (dpi). Macrophages are classified into 10 subpopulations with transcriptome among...
While the seroprevalence of SARS-CoV-2 in healthy people does not differ significantly among age groups, those aged 65 years or older exhibit strikingly higher COVID-19 mortality compared to younger individuals. To further understand differing manifestations patients different ages, three groups ferrets are infected with SARS-CoV-2. Although is isolated from all regardless age, (≥3 old) show viral loads, longer nasal virus shedding, and more severe lung inflammatory cell infiltration,...
During the 2021/2022 winter season, we isolated highly pathogenic avian influenza (HPAI) H5N1 viruses harbouring an amino acid substitution from Asparagine(N) to Aspartic (D) at residue 193 of hemagglutinin (HA) receptor binding domain (RBD) migratory birds in South Korea. Herein, investigated characteristics N193D HA-RBD A/CommonTeal/Korea/W811/2021[CT/W811] virus by using recombinant engineered via reverse genetics (RG). A affinity assay revealed that CT/W811 increases α2,6 sialic...
Dabie bandavirus (severe fever with thrombocytopenia syndrome virus [SFTSV]) induces an immunopathogenic disease a high fatality rate; however, the mechanisms underlying its clinical manifestations are largely unknown. In this study, we applied targeted proteomics and single-cell transcriptomics to examine differential immune landscape in SFTS patient blood. Serum immunoprofiling identified low-risk high-risk clusters of patients based on inflammatory cytokine levels, which corresponded...
Neuraminidase inhibitors (NAIs) have been widely used to control influenza virus infection, but their increased use could promote the global emergence of resistant variants. Although various mutations associated with NAI resistance identified, amino acid substitutions that confer multidrug undiminished viral fitness remain poorly understood. We therefore screened a known mutation(s) currently approved NAIs oseltamivir, zanamivir, and peramivir by assessing recombinant viruses mutant...
MicroRNAs (miRNAs) are known to regulate various biological processes, including expression of cellular gene and virus-induced inflammation. Recently, studies have indicated that some miRNAs could influenza virus replication. Due differential sensitivities A strains different species (avian mammalian), variations in host responses may be observed. Therefore, we investigated compared the differences global miRNA mouse lungs infected with wild type low pathogenicity A/Aquatic...
A novel genotype of H5N6 influenza viruses was isolated from migratory birds in South Korea during November 2016. Domestic outbreaks this virus were associated with die-offs wild near reported poultry cases Chungbuk province, central Korea. Genetic analysis and animal studies demonstrated that the Korean are highly pathogenic avian (HPAI) these reassortants at least three different subtypes (H5N6, H4N2 H1N1).
Omicron has become the globally dominant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant, creating additional challenges due to its ability evade neutralization. Here, we report that neutralizing antibodies against variants are undetected following COVID-19 infection with ancestral or past SARS-CoV-2 variant viruses after two-dose mRNA vaccination. Compared vaccination, a three-dose vaccination course induces broad antibody responses improved durability different...
Highly pathogenic avian influenza (HPAI) A(H5N6) and A(H5N8) virus infections resulted in the culling of more than 37 million poultry Republic Korea during 2016/17 winter season. Here we characterize two representative viruses, A/Environment/Korea/W541/2016 [Em/W541(H5N6)] A/Common Teal/Korea/W555/2017 [CT/W555(H5N8)], evaluate their zoonotic potential various animal models. Both Em/W541(H5N6) CT /W555(H5N8) are novel reassortants derived from gene pools wild bird viruses present migratory...
Various treatments and agents had been reported to inactivate RNA viruses. Of these, thermal inactivation is generally considered an effective cheap method of sample preparation for downstream assays. The purpose this study establish a safe SARS-CoV-2 without compromising the amount amplifiable viral genome necessary clinical diagnoses. In study, we demonstrate infectivity genomic stability SARSCoV- 2 by at both 56°C 65°C. results substantiate that viable readily inactivated when incubated...
Dabie bandavirus (DBV) is an emerging tick-borne virus that causes severe fever with thrombocytopenia syndrome (SFTS) in infected patients. Human SFTS symptoms progress from fever, fatigue, and muscle pain to the depletion of white blood cells platelets fatality rates up 30%. The recent spread its vector tick over 20 states United States increases potential for outbreaks beyond East Asia. Thus, development vaccine control this rapidly a high priority. In study, we applied self-assembling...
Abstract In 2009, the global outbreak of an influenza pandemic emphasized need for effective vaccine adjuvant. this study, we examined efficacy poly-γ-glutamic acid/chitosan (PC) nanogel as adjuvant vaccine. PC significantly enhanced antigen-specific cross-presentation and cytotoxic T lymphocyte (CTL) activity. Compared with alum, protective H1N1 (pH1N1) was substantially increased by nanogel, hemagglutination-inhibition titers, CTL activity, earlier virus clearance after homologous...
Black ginseng (BG, CJ EnerG), prepared via nine repeated cycles of steaming and drying fresh ginseng, contains more accessible acid polysaccharides smaller less polar ginsenosides than red (RG) processed only once. Because RG exhibits the ability to increase host protection against viral respiratory infections, we investigated antiviral effects BG. Mice were orally administered either BG or extract at 10 mg/kg bw daily for two weeks. then infected with a A(H1N1) pdm09 (A/California/04/2009)...
ABSTRACT The migratory waterfowl of the world are considered to be natural reservoir influenza A viruses. Of 16 hemagglutinin subtypes avian viruses, H6 subtype is commonly perpetuated in its hosts and concern due potential a precursor highly pathogenic viruses by reassortment. During routine surveillance, we isolated an unconventional H6N5 virus. Experimental infection mice revealed that this isolate replicated efficiently lungs, subsequently spread systemically, caused lethality. also...
We investigated influenza A(H5N6) viruses from migratory birds in Chungnam and Gyeonggi Provinces, South Korea following a reported die-off of poultry nearby provinces November 2017. Genetic analysis virulence studies chickens ducks identified our isolate December 2017 as novel highly pathogenic avian virus. It resulted reassortment between the virulent H5N8 strain with N6 gene low-pathogenic H3N6 virus Netherlands.