A5074180301- Influenza Virus Research Studies
- Respiratory viral infections research
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
- Solid-state spectroscopy and crystallography
- Plant Virus Research Studies
- Inorganic Fluorides and Related Compounds
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Perovskite Materials and Applications
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
Department of Agriculture
2023
Chungbuk National University
2018-2022
The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role diagnostics in impediment further spread transmission. With recent emergence novel SARS-CoV-2, availability rapid, sensitive, reliable diagnostic methods is essential for disease control. Hence, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific detection SARS-CoV-2. primer sets RT-LAMP were designed target nucleocapsid gene viral RNA, displayed limit 102...
Abstract Background In addition to seasonal influenza viruses recently circulating in humans, avian (AIVs) of H5N1, H5N6 and H7N9 subtypes have also emerged demonstrated human infection abilities with high mortality rates. Although viral infections are usually diagnosed using isolation serological/molecular analyses, the cost, accessibility, availability these methods may limit their utility various settings. The objective this study was develop optimized a multiplex detection system for...
Due to the broad spread, incidence, and genetic divergence of enteroviruses (EVs), it has been challenging deal with this virus that causes severe human diseases, including aseptic meningitis, myocarditis, encephalitis, poliomyelitis. Therefore, an efficient universal cloning system for reverse genetics highly divergent EVs contributes understanding viral pathology molecular mechanisms evolution.
Novel highly pathogenic avian influenza (HPAI) H5Nx viruses are predominantly circulating worldwide, with an increasing potential threat of outbreak in humans. It remains largely unknown how the stably maintained HPAI H5N1 suddenly altered its neuraminidase (NA) to other NA subtypes, which resulted emergence and evolution viruses. Here, we found that a combination four specific amino acid (AA) substitutions (S123P-T156A-D183N- S223 R) hemagglutinin (HA) protein consistently observed markedly...
Laninamivir (LAN) is a long-acting neuraminidase (NA) inhibitor (NAI) with similar binding profile in the influenza NA enzyme active site as those of other NAIs, oseltamivir (OS), zanamivir (ZAN), and peramivir, may share common resistance markers these NAIs. We screened viruses substitutions previously found during OS ZAN selection avian (AIVs) N3 to N9 subtypes for LAN susceptibility. Of 72 substitutions, 19 conferred LAN, which ranged from 11.2- 549.8-fold-decreased inhibitory activity...
The threat posed by coronaviruses to human health has necessitated the development of a highly specific and sensitive viral detection method that could differentiate between currently circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) other SARS-related (SARSr-CoVs). In this study, we developed peptide nucleic acid (PNA)-based real-time quantitative polymerase chain reaction (RT-qPCR) assay targeting N gene efficiently discriminate SARS-CoV-2 from SARSr-CoVs in clinical...
The reverse genetics (RG) system of influenza A viruses is well established. However, the conventional sequence-dependent method for cloning genome segments time-consuming and requires multiple processes (eg. enzyme digestion ligation) exhibits low efficiency compared to sequence-independent method. In this study, we improved into pHW2000 vector an RG by incorporating a circular polymerase extension (CPEC) approach which only 2 steps (reverse transcription one-pot CPEC-PCR) takes about 4...
An amendment to this paper has been published and can be accessed via the original article.