A5036481835- Influenza Virus Research Studies
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Immune Response and Inflammation
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- COVID-19 epidemiological studies
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Protein Structure and Dynamics
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Vector-borne infectious diseases
- Long-Term Effects of COVID-19
Chungbuk National University
2015-2025
Vrije Universiteit Brussel
2023
Medical Research Institute
2013-2021
Cheongju University
2013-2021
The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role diagnostics in impediment further spread transmission. With recent emergence novel SARS-CoV-2, availability rapid, sensitive, reliable diagnostic methods is essential for disease control. Hence, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific detection SARS-CoV-2. primer sets RT-LAMP were designed target nucleocapsid gene viral RNA, displayed limit 102...
Background. Although Middle East Respiratory Syndrome coronavirus (MERS-CoV) is characterized by a risk of nosocomial transmission, the detailed mode transmission and period virus shedding from infected patients are poorly understood. The aims this study were to investigate potential role environmental contamination MERS-CoV in healthcare settings define viable MERS patients.
Abstract Background In addition to seasonal influenza viruses recently circulating in humans, avian (AIVs) of H5N1, H5N6 and H7N9 subtypes have also emerged demonstrated human infection abilities with high mortality rates. Although viral infections are usually diagnosed using isolation serological/molecular analyses, the cost, accessibility, availability these methods may limit their utility various settings. The objective this study was develop optimized a multiplex detection system for...
ABSTRACT Adaptation of influenza A viruses to a new host species usually involves the mutation one or more eight viral gene segments, and molecular basis for range restriction is still poorly understood. To investigate changes that occur during adaptation low-pathogenic avian virus subtype commonly isolated from migratory birds mammalian host, we serially passaged avirulent wild-bird H5N2 strain A/Aquatic bird/Korea/W81/05 (W81) in lungs mice. The resulting mouse-adapted (ma81) was highly...
Due to the limitation of rapid development specific antiviral drug or vaccine for novel emerging viruses, an accurate and diagnosis is a key manage virus spread. We developed efficient method with high specificity Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT-LAMP). A set six LAMP primers (F3, B3, FIP, BIP, LF (Loop-F), LB (Loop-B) were designed using sequence nucleocapsid (N) gene optimized...
As the SARS-CoV-2 pandemic remains uncontrolled owing to continuous emergence of variants concern, there is an immediate need implement most effective antiviral treatment strategies, especially for risk groups. Here, we evaluated therapeutic potency nirmatrelvir, remdesivir and molnupiravir, their combinations in infected K18-hACE2 transgenic mice. Systemic mice with each drug (20 mg/kg) resulted slightly enhanced efficacy yielded increased life expectancy only about 20-40% survival....
Dabie bandavirus (severe fever with thrombocytopenia syndrome virus [SFTSV]) induces an immunopathogenic disease a high fatality rate; however, the mechanisms underlying its clinical manifestations are largely unknown. In this study, we applied targeted proteomics and single-cell transcriptomics to examine differential immune landscape in SFTS patient blood. Serum immunoprofiling identified low-risk high-risk clusters of patients based on inflammatory cytokine levels, which corresponded...
Recurrent spillovers of α- and β-coronaviruses (CoV) such as severe acute respiratory syndrome (SARS)-CoV, Middle East syndrome-CoV, SARS-CoV-2, possibly human CoV have caused serious morbidity mortality worldwide. In this study, six receptor-binding domains (RBDs) derived from β-CoV that are considered to originated animals cross-infected humans were linked a heterotrimeric scaffold, proliferating cell nuclear antigen (PCNA) subunits, PCNA1, PCNA2, PCNA3. They assemble create stable mosaic...
As the pandemic (H1N1) 2009 influenza virus continues to infect human populations globally, reports on epidemiologically linked animal infections are also rise. Since December 2009, 2009-like viruses have been isolated in pigs from different swine farms of South Korea. Genetic and phylogenetic analyses viral segments demonstrated several events human-to-swine transmission with no apparent signs reassortment. These were supported by serological surveillance pig sera collected April December,...
Neuraminidase inhibitors (NAIs) have been widely used to control influenza virus infection, but their increased use could promote the global emergence of resistant variants. Although various mutations associated with NAI resistance identified, amino acid substitutions that confer multidrug undiminished viral fitness remain poorly understood. We therefore screened a known mutation(s) currently approved NAIs oseltamivir, zanamivir, and peramivir by assessing recombinant viruses mutant...
MicroRNAs (miRNAs) are known to regulate various biological processes, including expression of cellular gene and virus-induced inflammation. Recently, studies have indicated that some miRNAs could influenza virus replication. Due differential sensitivities A strains different species (avian mammalian), variations in host responses may be observed. Therefore, we investigated compared the differences global miRNA mouse lungs infected with wild type low pathogenicity A/Aquatic...
Several subtypes of avian influenza viruses (AIVs) are emerging as novel human pathogens, and the frequency related infections has increased in recent years. Although neuraminidase (NA) inhibitors (NAIs) only class antiviral drugs available for therapeutic intervention AIV-infected patients, studies on NAI resistance among AIVs have been limited, markers poorly understood. Previously, we identified unique substitutions N3, N7, N9 NA subtypes. Here, report profiles that confer N4, N5, N6, N8...
ABSTRACT Due to dual susceptibility both human and avian influenza A viruses, pigs are believed be effective intermediate hosts for the spread production of new viruses with pandemic potential. In early 2008, two swine H5N2 were isolated from our routine surveillance in Korea. The sequencing phylogenetic analysis surface proteins revealed that Sw/Korea/C12/08 Sw/Korea/C13/08 derived Eurasian lineage. However, although isolate is an entirely avian-like virus, avian-swine-like reassortant PB2,...
Gene mutations and reassortment are key mechanisms by which influenza A virus acquires virulence factors. To evaluate the role of viral polymerase replication machinery in producing virulent pandemic (H1N1) 2009 viruses, we generated various point mutants (PB2, 627K/701N; PB1, expression PB1-F2 protein; PA, 97I) reassortant viruses with sources reverse genetics. Although produced no significant change pathogenicity, between A/California/04/09 (CA04, H1N1) current human animal variants...
Highly pathogenic avian influenza (HPAI) A(H5N6) and A(H5N8) virus infections resulted in the culling of more than 37 million poultry Republic Korea during 2016/17 winter season. Here we characterize two representative viruses, A/Environment/Korea/W541/2016 [Em/W541(H5N6)] A/Common Teal/Korea/W555/2017 [CT/W555(H5N8)], evaluate their zoonotic potential various animal models. Both Em/W541(H5N6) CT /W555(H5N8) are novel reassortants derived from gene pools wild bird viruses present migratory...
<title>Abstract</title> SARS-CoV-2 variants resistant to current antivirals remain a significant threat, particularly in high-risk patients. Although nirmatrelvir and ensitrelvir both target the viral 3CL protease (M<sup>pro</sup>), their distinct susceptibility profiles may allow alternative therapeutic approaches. Here, we identified novel deletion mutation at glycine 23 (Δ23G) M<sup>pro</sup> that conferred substantial resistance (~ 35-fold) while paradoxically increasing 8-fold). This...
Efficient worldwide swine surveillance for influenza A viruses is urgently needed; the emergence of a novel reassortant pandemic H1N1 (pH1N1) virus in 2009 demonstrated that can be direct source and potential prevalent populations must monitored. We used ferret model to assess pathogenicity transmissibility predominant Korean triple-reassortant (TRSw) H1N2 H3N2 genetically related North American strains. Although most TRSw were moderately pathogenic, one [A/Swine/Korea/1204/2009; Sw/1204...
Since the first emergence of SARS-CoV-2 variant in late 2020, novel variants have been continuously introduced to human population, causing severe public health threats. In general, because these acquire new genetic mutation/s, it is critical analyze biological function viruses that such mutations can confer.
Understanding the mammalian pathogenesis and interspecies transmission of HPAI H5N8 virus hinges on mapping its adaptive markers. We used deep sequencing to track these markers over five passages in murine lung tissue. Subsequently, we evaluated growth, selection, RNA load eight recombinant viruses with By leveraging an integrated non-linear regression model, quantitatively determined influence adaptation, expression hosts. Furthermore, our findings revealed that interplay can lead...
The vaccination program against the 2009 pandemic H1N1 influenza virus (2009 H1N1) provided a unique opportunity to determine if immune responses vaccine were affected by recent, prior seasonal virus. In present study, we studied in subjects who either received within 3 months or did not. Following vaccination, previously given exhibited significantly lower antibody responses, as determined hemagglutination inhibition assay, than had not vaccination. This result is compatible with phenomenon...