A5078918772- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Malaria Research and Control
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Pain Mechanisms and Treatments
- HIV Research and Treatment
- Botulinum Toxin and Related Neurological Disorders
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- Respiratory viral infections research
- Neurological disorders and treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Trypanosoma species research and implications
- Primate Behavior and Ecology
- Hair Growth and Disorders
- Research on Leishmaniasis Studies
- Dermatologic Treatments and Research
- Vagus Nerve Stimulation Research
- Animal Disease Management and Epidemiology
- Nicotinic Acetylcholine Receptors Study
- interferon and immune responses
- Facial Rejuvenation and Surgery Techniques
Institut Pasteur Korea
2011-2016
Chungnam National University
2006-2011
University of the Philippines Diliman
2005
Nagasaki University
2005
St. Luke's Medical Center
2003-2005
Chikungunya virus (CHIKV) is a mosquito-borne arthrogenic alphavirus that causes acute febrile illness in humans accompanied by joint pains and many cases, persistent arthralgia lasting weeks to years. The re-emergence of CHIKV has resulted numerous outbreaks the eastern hemisphere, threatens expand foreseeable future. Unfortunately, no effective treatment currently available. present study reports use resazurin cell-based high-throughput assay, an image-based high-content assay identify...
To demonstrate the differences of clinical features and hematologic abnormalities between dengue fever (DF) hemorrhagic (DHF), 359 pediatric patients admitted St. Luke's Medical Center in Quezon City, 1999 2001 Metro Manila, adjoining provinces Philippines, with a laboratory-confirmed virus infection were evaluated. One third had DHF, most these without shock. Restlessness, epistaxis, abdominal pain more associated DHF. The platelet count was significantly lower DHF group than DF before...
Serum samples from 54 monkeys were collected healthy individuals in a monkey farm Luzon island, Philippines, 1999, and examined by IgM-capture ELISA indirect IgG for the presence of dengue (DEN), Japanese encephalitis (JE) chikungunya (CHIK) viruses. The positive rates IgM 3.7, 35.2 14.8% against DEN, JE CHIK, respectively. Higher obtained when was used: 100% flaviviruses (JE or DEN) 59.3% CHIK virus. results indicate high prevalence flavivirus infections such as lesser virus infections,...
Human African trypanosomiasis (HAT) is a vector-transmitted tropical disease caused by the protozoan parasite Trypanosoma brucei. High-throughput screening (HTS) of small-molecule libraries in whole-cell assays one most frequently used approaches drug discovery for infectious diseases. To aid efforts HAT, SYBR Green assay was developed T. brucei 384-well format. This semi-automated cost- and time-effective, robust, reproducible. The compared to resazurin library 4000 putative kinase...
Although the public health impact of dengue is increasing rapidly, mechanism thrombocytopenia in this disease remains unknown. To elucidate mechanism, relationship between platelet-associated IgG (PAIgG) and platelet count 53 patients acute phase secondary virus infection was investigated a prospective-hospital-based study. A significant inverse correlation two parameters found these patients, while no observed healthy volunteers. The low baseline counts during 12 with significantly...
Dengue virus is a mosquito-borne flavivirus that has large impact in global health. It considered as one of the medically important arboviruses, and developing preventive or therapeutic solution remains top priority medical scientific community. Drug discovery programs for potential dengue antivirals have increased dramatically over last decade, largely part to introduction high-throughput assays. In this study, we developed an image-based high-throughput/high-content assay (HT/HCA) using...
Background Tumor innervation has been shown to be utilized by some solid cancers support tumor initiation, growth, progression, and metastasis, as well confer resistance immune checkpoint blockade through suppression of antitumor immunologic responses. Since botulinum neurotoxin type A1 (BoNT/A1) blocks neuronal cholinergic signaling, its potential use an anticancer drug in combination with anti-PD-1 therapy was investigated four different syngeneic mouse models.Methods Mice implanted breast...
Hyaluronic acid (HA) is the most common injectable dermal filler used for soft-tissue augmentation, and can be removed non-surgically by directly injecting hyaluronidase. In this study, hyaluronidase-mediated degradation of different types HA fillers implanted subcutaneously at back hairless mice having residence time four days or three months were compared.Two sites female with 0.1-mL one seven (NLL, NL, NDL, NVL, ND, JUVX+, RESLYFT) injected 30 IU 60 hyaluronidase per after reaching a 4 91...
BACKGROUND A new complexing protein-free botulinum toxin Type (CBoNT) with the same mechanism of action as complex onabotulinumtoxinA (OBoNT) and incobotulinumtoxinA (IBoNT) was recently developed. OBJECTIVE To compare local paresis chemodenervation efficacy 3 different preparations in mice. MATERIALS AND METHODS Efficacy duration CBoNT, OBoNT, IBoNT after a single intramuscular injection to right gastrocnemius evaluated by digit abduction score (DAS) compound muscle potential (CMAP) assays....
Botulinum neurotoxin type A (BoNT/A) causes muscle paralysis by blocking cholinergic signaling at neuromuscular junctions and is widely used to temporarily correct spasticity-related disorders deformities. The paralytic effects of BoNT/A are time-limited require repeated injections regular intervals achieve long-term therapeutic benefits. Differences in the level duration effectivity among various products can be attributed their unique manufacturing processes, formulation,...
Dengue virus (DV) is a mosquito-borne pathogen capable of infecting multiple target organs in the human host. Infection with any one four dengue serotypes can cause an acute febrile illness known as Fever (DF), while subsequent infection heterologous serotype or highly virulent strain result more severe and sometimes lethal Hemorrhagic (DHF) Shock Syndrome (DSS). According to World Health Organization, approximately 2.5 billion people live endemic countries, estimated 50 million cases...
Due to the similar symptoms with dengue fever (DF) and hemorrhagic (DHF), Chikungunya (CHIK) virus infections were diagnosed as DF or DHF. In this study, we examined more than 300 serum samples collected from dengue-suspected patients in San Lazaro Hospital St. Luke's Medical Center. Reverse transcriptase-polymerase chain reaction (RT-PCR) antigen sandwich enzyme-linked immunosorbent assay (ELISA) applied for CHIK detection. IgM-indirect immunofluorescent antibody test (IgM-IFA), IgM capture...