A5053282932- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Catalytic Processes in Materials Science
- Catalysts for Methane Reforming
- Catalysis and Oxidation Reactions
- Catalysis and Hydrodesulfurization Studies
- Viral Infections and Outbreaks Research
- Electrocatalysts for Energy Conversion
- Vector-borne infectious diseases
- Migration, Refugees, and Integration
- Malaria Research and Control
- Zeolite Catalysis and Synthesis
- Virology and Viral Diseases
- Plant Virus Research Studies
- Dengue and Mosquito Control Research
- HIV Research and Treatment
- Hydraulic Fracturing and Reservoir Analysis
- Migration and Labor Dynamics
- Social and Intergroup Psychology
- Fuel Cells and Related Materials
- Mesoporous Materials and Catalysis
- Viral Infectious Diseases and Gene Expression in Insects
- Electrodeposition and Electroless Coatings
- Vibrio bacteria research studies
- Vector-Borne Animal Diseases
Osaka University of Human Sciences
2022-2025
IRYO Sosei University
2024
Osaka University
1969-2022
Kyoto University
2022
Rikkyo University
2020-2021
Kogakuin University
2008-2020
Seikei University
2017-2020
Tohoku University
1979-2018
Eneos (Japan)
2014-2018
Japan Society for the Promotion of Science London
2018
In this study, the complete genomic sequence of chikungunya virus (CHIK; S27 African prototype) was determined and presence an internal polyadenylation [I-poly(A)] site confirmed within 3′ non-translated region (NTR) strain. The genome 11805 nucleotides in length, excluding 5′ cap nucleotide, I-poly(A) tract poly(A) tail. It comprised two long open reading frames that encoded non-structural (2474 amino acids) structural polyproteins (1244 acids). genetic location proteins predicted by...
Four primer pairs were selected on the basis of published sequence data four dengue virus serotypes so that each unique target size could be amplified for serotype by polymerase chain reaction. The procedure consists (i) RNA preparation, (ii) reverse transcription, and (iii) reaction, all which completed within 2 h in a single tube specimen. revealed ethidium bromide-stained agarose gel electrophoresis was serotype, using infected culture fluid isolates from fever or hemorrhagic patients...
Abstract A reverse transcription‐polymerase chain reaction (RT‐PCR) was developed for the detection of Chikungunya virus infection. Based on nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes , two primer sets were designed. Total RNA extracted from cell culture fluid Aedes albopictus C6/36 cells inoculated with S27 prototype virus, isolated in Tanzania 1953, Malaysian strains (MALh0198, MALh0298, MALh0398), Malaysia 1998. For both samples, expected 354‐ 294‐base pair (bp) cDNA...
A case of tick-borne encephalitis (TBE) has not been reported for many years in Japan, although a serological survey sera from domestic animals suggested the presence TBE foci Hokkaido, northern island Japan. Studies were conducted to prove an endemic focus virus Japan by means serology and isolation. In October 1993 severe dairy farmer's wife was diagnosed as TBE. Serological examination paired serum specimens showed rise neutralization antibody titer Russian spring summer virus....
Abstract Reverse transcriptase‐polymerase chain reaction (RT‐PCR) on 24 cerebrospinal fluid (CSF) specimens collected between February and August 1992 detected genome sequence of West Nile (WN) virus in 8 Japanese encephalitis (JE) a single specimen. The results, combined with the data by IgM‐ELISA CSF indicated that significant proportion acute cases Karachi, Pakistan, were caused WN infection, while JE small fraction.
The mechanism of cell death induced by West Nile virus (WNV), a causative agent human febrile syndrome and encephalitis, was investigated. WNV-infected K562 Neuro-2a cells manifested the typical features apoptosis, including shrinkage, chromatin condensation subdiploid DNA content flow cytometry. fragmentation into nucleosomal size changes in outer membrane phospholipid composition were also observed cells. UV-inactivated failed to induce above-mentioned characteristics, suggesting that...
To demonstrate the differences of clinical features and hematologic abnormalities between dengue fever (DF) hemorrhagic (DHF), 359 pediatric patients admitted St. Luke's Medical Center in Quezon City, 1999 2001 Metro Manila, adjoining provinces Philippines, with a laboratory-confirmed virus infection were evaluated. One third had DHF, most these without shock. Restlessness, epistaxis, abdominal pain more associated DHF. The platelet count was significantly lower DHF group than DF before...
The NS3 protein of Japanese encephalitis virus (JEV) contains motifs typical RNA helicase/NTPase but no helicase activity has been reported for this protein. To identify and characterize the JEV NS3, a truncated form with His-tag was expressed in Escherichia coli purified. purified showed an activity, which dependent on divalent cations ATP. An Asp-285-to-Ala substitution motif II abolished ATPase activities. These results indicate that C-terminal 457 residues are sufficient to exhibit NS3.
It was found that hydrogen recovery in a palladium membrane reactor is depressed by the back-permeation of from perm-side to reforming side near inlet reactor, where partial pressure on lower than because reaction has just started. To diminish this phenomenon, three methods, (1) use sweep gas, (2) premixing into feed, and (3) extension catalyst bed, were examined simulation as well experimentally. As result, it shown first method, if one uses steam useful fuel cell system requiring...
Serum samples from 54 monkeys were collected healthy individuals in a monkey farm Luzon island, Philippines, 1999, and examined by IgM-capture ELISA indirect IgG for the presence of dengue (DEN), Japanese encephalitis (JE) chikungunya (CHIK) viruses. The positive rates IgM 3.7, 35.2 14.8% against DEN, JE CHIK, respectively. Higher obtained when was used: 100% flaviviruses (JE or DEN) 59.3% CHIK virus. results indicate high prevalence flavivirus infections such as lesser virus infections,...