A5100761902- Influenza Virus Research Studies
- Energy and Environmental Systems
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Engineering Applied Research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Animal Virus Infections Studies
- Food Quality and Safety Studies
- Sphingolipid Metabolism and Signaling
- Vector-Borne Animal Diseases
- Wireless Communication Networks Research
- Telecommunications and Broadcasting Technologies
- Agriculture, Soil, Plant Science
- Advancements in Transdermal Drug Delivery
- Advanced Wireless Communication Techniques
- Long-Term Effects of COVID-19
- IPv6, Mobility, Handover, Networks, Security
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Refrigeration and Air Conditioning Technologies
Institute for Basic Science
2022-2025
University of Alabama at Birmingham
2010-2023
Korea University
2023
Korea Electronics Technology Institute
2023
Chungbuk National University
2013-2022
Electronics and Telecommunications Research Institute
2005-2022
Korea Institute of Industrial Technology
2019-2022
Faculty of 1000 (United States)
2021
Sunrise Medical (United States)
2021
Seoul National University of Science and Technology
2007-2020
The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role diagnostics in impediment further spread transmission. With recent emergence novel SARS-CoV-2, availability rapid, sensitive, reliable diagnostic methods is essential for disease control. Hence, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific detection SARS-CoV-2. primer sets RT-LAMP were designed target nucleocapsid gene viral RNA, displayed limit 102...
Abstract Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting receptor binding domain (RBD) of viral spike protein via antibody library constructed from peripheral blood mononuclear cells a convalescent patient. The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including D614G variant without antibody-dependent enhancement effect. Complex crystal...
ObjectivesThe aim was to determine whether various clinical specimens obtained from COVID-19 patients contain the infectious virus.MethodsTo demonstrate viable virus, we collected naso/oropharyngeal swabs and saliva, urine stool samples five performed a quantitative polymerase chain reaction (qPCR) assess viral load. Specimens positive with qPCR were subjected virus isolation in Vero cells. We also used intranasally inoculate ferrets evaluated titres nasal washes on 2, 4, 6 8 days post...
Background. Although Middle East Respiratory Syndrome coronavirus (MERS-CoV) is characterized by a risk of nosocomial transmission, the detailed mode transmission and period virus shedding from infected patients are poorly understood. The aims this study were to investigate potential role environmental contamination MERS-CoV in healthcare settings define viable MERS patients.
Due to the urgent need of a therapeutic treatment for coronavirus (CoV) disease 2019 (COVID-19) patients, number FDA-approved/repurposed drugs have been suggested as antiviral candidates at clinics, without sufficient information. Furthermore, there extensive debates over their effectiveness and safety against severe acute respiratory syndrome CoV 2 (SARS-CoV-2), suggesting that rapid preclinical animal studies are required identify potential human trials. To this end, efficacies...
Abstract Background In addition to seasonal influenza viruses recently circulating in humans, avian (AIVs) of H5N1, H5N6 and H7N9 subtypes have also emerged demonstrated human infection abilities with high mortality rates. Although viral infections are usually diagnosed using isolation serological/molecular analyses, the cost, accessibility, availability these methods may limit their utility various settings. The objective this study was develop optimized a multiplex detection system for...
The endemicity of highly pathogenic avian influenza (HPAI) A(H5N1) viruses in Asia has led to the generation reassortant H5 strains with novel gene constellations. A newly emerged HPAI A(H5N8) virus caused poultry outbreaks Republic Korea 2014. Because emerging high-pathogenicity continue pose public health risks, it is imperative that their pathobiological properties be examined. Here, we characterized A/mallard duck/Korea/W452/2014 (MDk/W452(H5N8)), a representative virus, and evaluated...
Abstract Asthma is a chronic inflammatory disease process involving the conductive airways of human lung. The dysregulated response in this may involve multiple cell-cell interactions mediated by signaling molecules, including lipid mediators. Extracellular vesicles (EVs) are membrane particles that now recognized as critical mediators communication. Here, we compared composition and presence specific airway EVs purified from bronchoalveolar lavage (BAL) fluid healthy controls asthmatic...
To investigate nationwide severe fever with thrombocytopenia syndrome virus (SFTSV) infection status, we isolated SFTSVs from patients suspected (SFTS) in 207 hospitals throughout South Korea between 2013 and April 2017. A total of 116 were 3137 SFTS-suspected patients, an overall 21.6% case fatality rate. Genetic characterization revealed that at least 6 genotypes co-circulating Korea, multiple reassortments among them. Of these, the genotype B-2 strains most prevalent, followed by F...
Background: Tacrolimus (TCR), also known as FK-506, is a biopharmaceutics classification system (BCS) class II drug that insoluble in water because of its high log P values. After dermal application, TCR remains the stratum corneum and passes through skin layers with difficulty. Purpose: The objectives this study were to develop evaluate solid lipid nanoparticles (SLNs) thermosensitive properties improve penetration retention. Methods: We prepared TCR-loaded (TCR-SLNs) different types...
Given the current coronavirus disease 2019 (COVID-19) pandemic, coinfection of severe acute respiratory syndrome 2 (SARS-CoV-2) and influenza A virus (IAV) is a major concern for public health. However, immunopathogenic events occurring with coinfections SARS-CoV-2 IAV remain unclear. Here, we report pathogenic immunological consequences H1N1 in K18-hACE2 transgenic mouse model. Compared single infection or IAV, not only prolonged primary period but also increased immune cell infiltration...
Cases of laboratory-confirmed SARS-CoV-2 reinfection have been reported in a number countries. Further, the level natural immunity induced by infection is not fully clear, nor it clear if primary protective against reinfection. To investigate potential association between serum antibody titres and SARS-CoV-2, ferrets with different levels NAb after were subjected to heterologous strain. All reinfected showed active virus replication upper respiratory gastro-intestinal tracts. However, high...
Abstract Few studies have used a longitudinal approach to describe the immune response SARS-CoV-2 infection. Here, we perform single-cell RNA sequencing of bronchoalveolar lavage fluid cells longitudinally obtained from SARS-CoV-2-infected ferrets. Landscape analysis lung microenvironment shows distinct changes in cell proportions and characteristics compared uninfected control, at 2 5 days post-infection (dpi). Macrophages are classified into 10 subpopulations with transcriptome among...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a causative agent of the CoV disease 2019 (COVID-19) pandemic, enters host cells via interaction its receptor-binding domain (RBD) spike protein with angiotensin-converting enzyme (ACE2). Therefore, RBD is promising vaccine target to induce protective immunity against SARS-CoV-2 infection.
While the seroprevalence of SARS-CoV-2 in healthy people does not differ significantly among age groups, those aged 65 years or older exhibit strikingly higher COVID-19 mortality compared to younger individuals. To further understand differing manifestations patients different ages, three groups ferrets are infected with SARS-CoV-2. Although is isolated from all regardless age, (≥3 old) show viral loads, longer nasal virus shedding, and more severe lung inflammatory cell infiltration,...
With the emergence of multiple predominant SARS-CoV-2 variants, it becomes important to have a comprehensive assessment their viral fitness and transmissibility. Here, we demonstrate that natural temperature differences between upper (33°C) lower (37°C) respiratory tract profound effects on replication Specifically, variants containing NSP12 mutations P323L or P323L/G671S exhibit enhanced RNA-dependent RNA polymerase (RdRp) activity at 33°C compared with 37°C high Molecular dynamics...
During the 2021/2022 winter season, we isolated highly pathogenic avian influenza (HPAI) H5N1 viruses harbouring an amino acid substitution from Asparagine(N) to Aspartic (D) at residue 193 of hemagglutinin (HA) receptor binding domain (RBD) migratory birds in South Korea. Herein, investigated characteristics N193D HA-RBD A/CommonTeal/Korea/W811/2021[CT/W811] virus by using recombinant engineered via reverse genetics (RG). A affinity assay revealed that CT/W811 increases α2,6 sialic...
We recently identified a previously unidentified sphingosine-1-phosphate (S1P) signaling mechanism that stimulates production of key innate immune element, cathelicidin antimicrobial peptide (CAMP), in mammalian cells exposed to external perturbations, such as UVB irradiation and other oxidative stressors provoke subapoptotic levels endoplasmic reticulum (ER) stress, independent the well-known vitamin D receptor-dependent mechanism. ER stress increases cellular ceramide one its distal...
Although the incidence of severe fever with thrombocytopenia syndrome virus (SFTSV) infection has increased from its discovery a mortality rate 10-20%, no effective vaccines are currently available. Here we describe development SFTSV DNA vaccine, immunogenicity, and protective efficacy. Vaccine candidates induce both neutralizing antibody response multifunctional SFTSV-specific T cell in mice ferrets. When vaccine efficacy is investigated aged-ferrets that recapitulate fatal clinical...
Dabie bandavirus (severe fever with thrombocytopenia syndrome virus [SFTSV]) induces an immunopathogenic disease a high fatality rate; however, the mechanisms underlying its clinical manifestations are largely unknown. In this study, we applied targeted proteomics and single-cell transcriptomics to examine differential immune landscape in SFTS patient blood. Serum immunoprofiling identified low-risk high-risk clusters of patients based on inflammatory cytokine levels, which corresponded...
Dyslipidemia is a major risk factor for development of several obesity-related diseases. The peroxisome proliferator-activated receptor α (PPARα) ligand-activated transcription that regulates energy metabolism. Previously, we reported 9-oxo-10,12-octadecadienoic acid (9-oxo-ODA) presented in fresh tomato fruits and acts as PPARα agonist. In addition to 9-oxo-ODA, developed 13-oxo-9,11-octadecadienoic (13-oxo-ODA), which an isomer present only juice. this study, explored the possibility...