A5050261329- Immunotherapy and Immune Responses
- Bone Metabolism and Diseases
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Biopolymer Synthesis and Applications
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Biosensors and Analytical Detection
- Endodontics and Root Canal Treatments
- Animal Virus Infections Studies
- Oral microbiology and periodontitis research
- Viral Infections and Vectors
- Immune Response and Inflammation
- Bone health and treatments
- Cancer Research and Treatments
- Animal Disease Management and Epidemiology
- Radiopharmaceutical Chemistry and Applications
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Bacillus and Francisella bacterial research
- Viral gastroenteritis research and epidemiology
Korea Research Institute of Bioscience and Biotechnology
2015-2024
Sea Lane Biotechnologies (United States)
2024
Seoul National University
2007-2023
Shenzhen Children's Hospital
2023
Korea University of Science and Technology
2022
Research Research (South Africa)
2021
Institute for Bioscience and Biotechnology Research
2021
Research Institute for Bioscience and Biotechnology
2021
Zero to Three
2019
Guangxi Center for Disease Prevention and Control
2010
Given the current coronavirus disease 2019 (COVID-19) pandemic, coinfection of severe acute respiratory syndrome 2 (SARS-CoV-2) and influenza A virus (IAV) is a major concern for public health. However, immunopathogenic events occurring with coinfections SARS-CoV-2 IAV remain unclear. Here, we report pathogenic immunological consequences H1N1 in K18-hACE2 transgenic mouse model. Compared single infection or IAV, not only prolonged primary period but also increased immune cell infiltration...
Health care workers continued to contract severe acute respiratory syndrome (SARS), even after barrier precautions were widely implemented.We explored the possible contribution of contaminated hospital surfaces SARS transmission by swabbing in 2 hospitals and testing swab samples reverse-transcriptase polymerase chain reaction (RT-PCR) viral culture.Twenty-six 94 tested positive for RNA. Swab secretions from each 4 patients examined RT-PCR, as 12 43 swabs patient rooms 10 47 other parts...
Abstract Recently, mRNA‐based therapeutics, including vaccines, have gained significant attention in the field of gene therapy for treating various diseases. Among mRNA delivery vehicles, lipid nanoparticles (LNPs) emerged as promising vehicles packaging and delivering with low immunogenicity. However, while has several advantages, efficiency stability LNPs remain challenging therapy. In this study, an ionizable helper cholesterol analog, 3β[L‐histidinamide‐carbamoyl] (Hchol) is developed...
Abstract In 2009, the global outbreak of an influenza pandemic emphasized need for effective vaccine adjuvant. this study, we examined efficacy poly-γ-glutamic acid/chitosan (PC) nanogel as adjuvant vaccine. PC significantly enhanced antigen-specific cross-presentation and cytotoxic T lymphocyte (CTL) activity. Compared with alum, protective H1N1 (pH1N1) was substantially increased by nanogel, hemagglutination-inhibition titers, CTL activity, earlier virus clearance after homologous...
Bone-resorbing osteoclasts are differentiated from macrophages (MΦ) by M-CSF and RANKL. MΦ can be mainly classified into M1 M2 MΦ, which proinflammatory anti-inflammatory, respectively, but little is known about their osteoclastogenic potential. Here, we investigated the potential of subtypes. When two subtypes were using RANKL, more potently than MΦ. generated with IL-4 or IL-10 also showed enhanced osteoclast differentiation compared induced IFN-γ lipopolysaccharide. In addition, robust...
Recently, a complex (X/C) hepatitis B virus (HBV) recombinant, first reported in 2000, was proposed as new genotype; although this refuted immediately because the strains differ by less than 8 % nucleotide distance from genotype C. Over 13.5 (38/281) of HBV isolates Long An cohort China were not assigned to specific genotype, using current genotyping tools analyse surface ORF sequences, and these have about 98 similarity X/C recombinants. To determine whether close identity extends...
The use of a good vaccine adjuvant may induce higher immunogenicity profile antigens. Here, we developed new by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the cross-reactive efficacy pandemic H1N1 (pH1N1) influenza antigen. PGA/Alum enhanced antigen delivery draining lymph nodes antigen-specific in mice using OVA as model It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity,...
Introduction Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19), had a major impact on both global health and economy. Numerous virus-neutralizing antibodies were developed against S1 subunit SARS-CoV-2 spike (S) protein to block viral binding host cells authorized for control COVID-19 pandemic. However, frequent mutations in enabled emergence immune evasive variants. To address these challenges, broadly neutralizing targeting...
The 4-1BB is a costimulatory molecule similar to the receptor activator of NF-kappaB ligand (RANKL), both which are key factors for differentiation osteoclasts and expressed mainly by activated T cells. shares common signaling pathways with RANK, suggesting potential role in osteoclastogenesis. In this study, (4-1BBL) osteoclastogenesis was investigated using 4-1BB(-/-) 4-1BB(+/+) mice. Osteoclast precursors normally express 4-1BBL after exposure RANKL, confirmed semi-quantitative RT-PCR...
Abstract Bone resorption can be caused by excessive differentiation and/or activation of bone‐resorbing osteoclasts. While microbe‐associated molecular patterns influence the and bone cells, little is known about role lipoteichoic acid (LTA), a major cell wall component Gram‐positive bacteria, in regulation metabolism. In this study, we investigated effect LTA on metabolism using wild‐type Staphylococcus aureus LTA‐deficient mutant strain. S. induced higher loss osteoclast than . isolated...
Approved COVID-19 vaccines primarily induce neutralizing antibodies targeting the receptor-binding domain (RBD) of SARS-CoV-2 spike (S) protein. However, emergence variants concern with RBD mutations poses challenges to vaccine efficacy. This study aimed design a next-generation that provides broader protection against diverse coronaviruses, focusing on glycan-free S2 peptides as candidates overcome low immunogenicity due N-linked glycans S antigen stalk, which can mask antibody responses....
Serum amyloid A is an acute phase protein that elevated under inflammatory conditions.Additionally, the serum levels of are associated with progression arthritis; thus, might be involved in regulation osteoclast differentiation.In present study, we examined effects on differentiation and function.When bone marrow-derived macrophages, as precursors, were stimulated presence M-CSF receptor activator nuclear factor-kB ligand, its boneresorption activity substantially inhibited.TLR2 was...
Foot-and-mouth disease (FMD) is a highly contagious and economically devastating affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A one of the most common causes outbreaks among seven FMDV serotypes, its serological diagnosis therefore important to confirm infection determine vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific via hybridoma systems using an inactivated (A22/Iraq/1964) found 4 monoclones (#29, #106, #108, #109) with high binding...
Abstract 3 H‐Sch 58235 was prepared at a specific activity of 29.1 Ci/mmol by Ir(COD)(Cy P)PyPF 6 catalysed exchange with tritium gas. 14 C‐Sch in three steps from p ‐hydroxy[ring‐U‐ C]benzaldehyde an overall radiochemical yield 21%. 13 C ‐Sch similarly ]benzaldehyde 41%. Copyright © 2002 John Wiley & Sons, Ltd.