A5052080260- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Agriculture, Soil, Plant Science
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Food Quality and Safety Studies
- Plant nutrient uptake and metabolism
- Rice Cultivation and Yield Improvement
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- GABA and Rice Research
- Flowering Plant Growth and Cultivation
- Microbial Metabolites in Food Biotechnology
- CRISPR and Genetic Engineering
- Plant Pathogens and Resistance
- Plant Gene Expression Analysis
- Light effects on plants
- Potato Plant Research
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Plant and Fungal Interactions Research
- Soybean genetics and cultivation
Korea Research Institute of Bioscience and Biotechnology
2014-2024
Korea University of Science and Technology
2020-2024
Systems Engineering Research Center
2014
Wonkwang University
2010
Yonsei Proteome Research Center
2009
Genomics Medicine (Ireland)
2008
Korea International Cooperation Agency
2007
Genome Research Foundation
2007
When plants are grown under saline conditions, photosynthetic activity decreases leading to reduced plant growth, leaf area, chlorophyll content and fluorescence. Seeds seedlings of radish (Raphanus sativus L.) were in NaCl solutions controlled greenhouse conditions. The concentrations complete nutrient 0 (control), 4.7, 9.4 14.1 dS m-1. salinity germination percentage also delayed the rate as salt level increased. Lengths fresh weights root shoot decreased with increasing concentration....
Abstract The pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused a public health emergency, and research on the development various types vaccines is rapidly progressing at an unprecedented speed internationally. Some have already been approved for emergency use are being supplied to people around world, but there still many ongoing efforts create new vaccines. Virus-like particles (VLPs) enable construction promising platforms in field vaccine development....
Summary Plants sense mechanical stimuli to recognise nearby obstacles and change their growth patterns adapt the surrounding environment. When roots encounter an obstacle, they rapidly bend away from impenetrable surface find edge of barrier. However, molecular mechanisms underlying root−obstacle avoidance are largely unknown. Here, we demonstrate that PIN ‐ FORMED ( )‐mediated polar auxin transport facilitates root bending during obstacle avoidance. We analysed two types after touched...
Isoflavonoids are a class of biologically active natural products that accumulate in soybean (Glycine max L.) seeds during development, play vital roles plant defense, and act as phytoestrogens with important human health benefits. Plant cell suspension cultures represent an excellent source secondary metabolites. We found methyl jasmonate (MJ) treatment increased isoflavone production cultures. To investigate the underlying mechanism, we examined expression structural genes (CHS6, CHS7,...
Tuberization is an important developmental process in potatoes, but it highly affected by environmental conditions. Temperature a major factor affecting tuberization, with high temperatures suppressing tuber development. However, the temporal aspects of thermo-responsive tuberization remain elusive. In this study, we show that FT homolog StSP6A suppressed temporally distinct regulatory pathways. Experiments using StSP6A-overexpressing plants post-transcriptional regulation plays role at...
Summary Nonhost resistance (NHR) is a robust plant immune response against non‐adapted pathogens. A number of nucleotide‐binding leucine‐rich repeat (NLR) proteins that recognize pathogens have been identified, although the underlying molecular mechanisms driving robustness NHR are still unknown. Here, we screened 57 effectors potato late blight pathogen Phytophthora infestans in nonhost pepper ( Capsicum annuum ) to identify avirulence effector candidates. Selected were tested 436...
Human beta-amyloid (Abeta) is believed to be one of the main components Alzheimer's disease, so reduction Abeta considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for with tandem repeats. Integration human gene into tomato genome and its transcription were detected by PCR Northern blot, respectively. Expression protein was confirmed western blot ELISA, then line expressing highest level selected vaccination. Mice...
The pepper Pvr4 protein encoding coiled-coil (CC) nucleotide-binding (NB) leucine-rich repeat (LRR) (NLR) confer hypersensitive response (HR) to potyviruses, including Pepper mottle virus (PepMoV), by recognizing the viral avirulence NIb. To figure out Pvr4-mediated HR mechanism, we analyzed signaling component genes and structure-function relationships of Pvr4, using chimeras deletion mutants in Nicotiana benthamiana. Molecular chaperone components HSP90, SGT1, RAR1 were required, while...
Peptidyl-prolyl isomerase-like 1 (PPIL1) is associated with the human spliceosome complex. However, its function in pre-mRNA splicing remains unclear. In this study, we show that Arabidopsis thaliana CYCLOPHILIN 18-2 (AtCYP18-2), a PPIL1 homolog, plays an essential role heat tolerance by regulating splicing. Under stress conditions, AtCYP18-2 expression was upregulated mature plants and GFP-tagged redistributed to nuclear cytoplasmic puncta. We determined interacts several complex BACT...
Reactive oxygen species (ROS) and calcium ions (Ca2+) are representative signals of plant wound responses. Wounding triggers cell fate transition in detached tissues induces de novo root organogenesis. While the hormonal regulation organogenesis has been widely studied, role early including ROS Ca2+ remains largely unknown.We identified that required for organogenesis, but have different functions Arabidopsis explants. The inhibition delayed development leaves. Examination auxin signaling...
Abstract In plants, heat stress induces changes in alternative splicing, including intron retention; these events can rapidly alter proteins or downregulate protein activity, producing nonfunctional isoforms inducing nonsense-mediated decay of messenger RNA (mRNA). Nuclear cyclophilins (CYPs) are accessory the spliceosome complexes multicellular eukaryotes. However, whether plant CYPs involved pre-mRNA splicing remain unknown. Here, we found that Arabidopsis thaliana CYP18-1 is necessary for...
Jerusalem artichoke (Helianthus tuberosus L.) has long been cultivated as a vegetable and source of fructans (inulin) for pharmaceutical applications in diabetes obesity prevention. However, transcriptomic genomic data remain scarce. In this study, Illumina RNA sequencing (RNA-Seq) was performed on samples from leaves, roots, stems two different tuber tissues (early late development). Data were used de novo assembly characterization the transcriptome. total 206,215,632 paired-end reads...
Alternative splicing (AS) is an important molecular mechanism by which single genes can generate multiple mRNA isoforms. We reported previously that, in Oryza sativa, the cyclophilin 19-4 (OsCYP19-4.1) transcript was significantly upregulated response to cold stress, and that transgenic plants were tolerant. Here we show under OsCYP19-4 produces eight variants intron retention exon skipping, resulting production of four distinct protein The AS isoforms exhibited different cellular...
Potato ( Solanum tuberosum L.) cultivation is threatened by various environmental stresses, especially disease. Genome editing technologies are effective tools for generating pathogen-resistant potatoes. Here, we established an efficient RNP-mediated CRISPR/Cas9 genome protocol in potato to develop Phytophthora infestans resistant mutants targeting the susceptibility gene, Signal Responsive 4 SR4 ), protoplasts. Mutations StSR4 were efficiently introduced into regenerated plants, with a...
CYP21-4 is a novel Golgi-localized cyclophilin protein involved in oxidative stress tolerance. Here, we generated transgenic plants overexpressing AtCYP21-4 and OsCYP21-4 potato rice, respectively. The stems roots of AtCYP21-4overexpressing were longer than those wild-type (WT) plants, which resulted heavier tubers. In vitro tuberization the also significantly greater tuber number weight, as well shorter time to microtuber formation. Similarly, OsCYP21-4overexpressing rice had higher...
Sophorae Radix ( Sophora flavescens Aiton) has long been used in traditional medicine East Asia due to the various biological activities of its secondary metabolites. Endogenous contents phenolic compounds (phenolic acid, flavonol, and isoflavone) main bioactive were analyzed based on qualitative HPLC analysis evaluated different organs at developmental stages. In total, 11 detected, composition roots aerial parts (leaves, stems, flowers) was significantly different. trans-Cinnamic acid p...