A5087433315- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Plant Reproductive Biology
- Cellular transport and secretion
- Plant tissue culture and regeneration
- Advanced Photocatalysis Techniques
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Consumer Perception and Purchasing Behavior
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Photonic and Optical Devices
- Advancements in PLL and VCO Technologies
- Plant Gene Expression Analysis
- Semiconductor materials and devices
- TiO2 Photocatalysis and Solar Cells
- Food Quality and Safety Studies
- Plant Pathogens and Resistance
- Phytochemicals and Antioxidant Activities
- Advanced Nanomaterials in Catalysis
- Transportation Planning and Optimization
- Genetic Mapping and Diversity in Plants and Animals
Kangwon National University
2018-2025
Samsung (South Korea)
2008-2025
Korea Basic Science Institute
2013-2024
Gwangju Institute of Science and Technology
2013-2023
Korea University of Science and Technology
2013-2023
Korea Research Institute of Bioscience and Biotechnology
2013-2023
Korea University
2017-2023
Ewha Womans University
2005-2022
Sejong University
2020-2022
Gwangju National University of Education
2020
A-Maize-ing Maize is one of our oldest and most important crops, having been domesticated approximately 9000 years ago in central Mexico. Schnable et al. (p. 1112 ; see the cover) present results sequencing B73 inbred maize line. The findings elucidate how became diploid after an ancestral doubling its chromosomes reveals transposable element movement activity recombination. Vielle-Calzada 1078 ) have sequenced Palomero Toluqueño ( landrace, a highland popcorn from Mexico, which, when...
Retrotransposons are the main components of eukaryotic genomes, representing up to 80% some large plant genomes. These mobile elements transpose via a “copy and paste” mechanism, thus increasing their copy number while active. Their accumulation is now accepted as factor genome size increase in higher eukaryotes, besides polyploidy. However, dynamics this process poorly understood. In study, we show that Oryza australiensis , wild relative Asian cultivated rice O. sativa has undergone recent...
Abstract Cpf1, a type V CRISPR effector, recognizes thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by single RNA (crRNA). Here we show that Cpf1 can be used as tool for DNA-free editing of plant genomes. We describe delivery recombinant proteins with in vitro transcribed or chemically synthesized target-specific crRNAs into protoplasts isolated from soybean wild tobacco. Designed are unique do not have similar sequences (≤3...
Rice was chosen as a model organism for genome sequencing because of its economic importance, small size, and syntenic relationship with other cereal species. We have constructed bacterial artificial chromosome fingerprint-based physical map the rice to facilitate whole-genome rice. Most ( approximately 90.6%) anchored genetically by overgo hybridization, DNA gel blot in silico anchoring. Genome data also were integrated into map. Comparison genetic maps reveals that recombination is...
The Rice Annotation Project Database (RAP-DB) was created to provide the genome sequence assembly of International Genome Sequencing (IRGSP), manually curated annotation sequence, and other genomics information that could be useful for comprehensive understanding rice biology. Since last publication RAP-DB, IRGSP has been revised reassembled. In addition, a large number rice-expressed tags have released, functional resources produced worldwide. Thus, we thoroughly updated our by manual...
The genome of an anonymous Korean male has been sequenced using a broad spread genomic techniques. This combinatorial approach allows for detailed characterization sequence and structural variation. first four individual genomes to have determined spanned three distinct ethnic groups: Yoruba African, northwest European (Craig Venter James Watson) Han Chinese. new work, together with another reported elsewhere, adds the Altaic grouping list. Human sequences so far individuals ancestry in...
Maize (Zea mays L.) is one of the most important cereal crops and a model for study genetics, evolution, domestication. To better understand maize genome organization to build framework sequencing, we constructed sequence-ready fingerprinted contig-based physical map that covers 93.5% genome, which 86.1% aligned genetic map. The contig contains 25,908 genic markers enabled us align nearly 73% anchored rice genome. distribution pattern expressed sequence tags correlates recombination. In...
Rab proteins are members of the Ras superfamily small GTP binding and play important roles in various intracellular trafficking steps. We investigated role Rha1, an Arabidopsis Rab5 homolog, protoplasts. In presence a dominant-negative mutant soluble vacuolar cargo such as sporamin:green fluorescent protein (Spo:GFP) aleurain like protein:GFP not delivered to central vacuole; instead, they accumulate diffuse or punctate staining pattern within cell. Spo:GFP at stains observed...
Approximately 185,000 Gossypium EST sequences comprising >94,800,000 nucleotides were amassed from 30 cDNA libraries constructed a variety of tissues and organs under range conditions, including drought stress pathogen challenges. These derived allopolyploid cotton ( hirsutum ; A T D genomes) as well its two diploid progenitors, arboreum (A genome) raimondii (D genome). ESTs assembled using the Program for Assembling Viewing (PAVE), resulting in 22,030 contigs 29,077 singletons (51,107...
In all eukaryotic cells, a membrane-trafficking system connects the post-Golgi organelles, such as trans-Golgi network (TGN), endosomes, vacuoles, and plasma membrane. This complex plays critical roles in several higher-order functions multicellular organisms. The TGN, one of important organelles for protein transport network, sorting station, where cargo proteins are directed to appropriate compartments. Unlike its animal yeast TGN has also been reported function like early endosomal...
Adaptor protein (AP) complexes are the predominant coat proteins of membrane vesicles in post-Golgi trafficking mammalian cells. Each AP complex contains a specific medium subunit, μ-adaptin, that selects cargo bearing sequence-specific sorting motifs. Much less is known about and their μ subunits plants. Because uncertain homology, μ-adaptins Arabidopsis have been designated muA through muD [Happel et al. (2004) Plant J 37(5):678–693]. Furthermore, only has assigned to complex, AP-3,...
The genes that encode the ethylene biosynthesis enzyme 1-aminocyclopropane-1-carboxylate oxidase (ACO) are thought to be involved in flower senescence. Hence, we investigated whether transcript levels of PhACO (PhACO1, PhACO3 and PhACO4) Petunia cv. Mirage Rose associated with production at different flowering stages. High were detected late stage linked high levels. PhACO1 was subsequently edited using CRISPR/Cas9 system, its role investigated. PhACO1-edited T0 mutant lines, regardless type...
We suggest an easy one-step underwater plasma method to generate nanoporous N-doped TiO2(N-TiO2) photocatalysts with high reactivity in the visible light range. The extreme energy input of this technology allowed synthesis, crystallization, doping, and porosity TiO2 by inducing continuous reaction highly energetic atomic molecular species within a few minutes. produced N-TiO2 demonstrated anatase-brookite polycrystalline structure substitutionally doped N atoms. doping led narrow bandgap,...
The small nanoparticle size and long-chain ligands in colloidal metal halide perovskite quantum dots (PeQDs) cause charge confinement, which impedes exciton dissociation carrier extraction PeQD solar cells, so they have low short-circuit current density Jsc , further increases their power conversion efficiency (PCE). Here, a re-assembling process (RP) is developed for nanocrystalline (PeNC) films made of nanocrystals to increase PeNC cells. RP crystallite eliminates ligands, thereby...
We have previously demonstrated that vacuolar sorting receptor (VSR) proteins are concentrated on prevacuolar compartments (PVCs) in plant cells. PVCs tobacco (Nicotiana tabacum) BY-2 cells multivesicular bodies (MVBs) as defined by VSR and the BP-80 reporter, where transmembrane domain (TMD) cytoplasmic tail (CT) sequences of sufficient specific for correct targeting reporter to PVCs. The genome Arabidopsis (Arabidopsis thaliana) contains seven proteins, but little is known about their...
In view of the immense value Brassica rapa in fields agriculture and molecular biology, multinational Genome Sequencing Project (BrGSP) was launched 2003 by five countries. The developing BrGSP has valuable resources for community, including a reference genetic map seed BAC sequences. Although initial B. linkage served as BrGSP, there ambiguity reconciling groups with ten chromosomes rapa. Consequently, assigned each to project members chromosome substitutes sequencing.We identified simple...
Abstract We report an effect involving hydrogen (H 2 )-plasma-treated nanoporous TiO (H-TiO ) photocatalysts that improve photocatalytic performance under solar-light illumination. H-TiO were prepared by application of plasma assynthesized (a-TiO without annealing process. Compared with the a-TiO , exhibited high anatase/brookite bicrystallinity and a porous structure. Our study demonstrated H is simple strategy to fabricate covering large surface area offers many active sites for extension...
Plants employ multiple cell-autonomous defense mechanisms to impede pathogenesis of microbial intruders. Previously we identified an exocytosis mechanism in Arabidopsis against pathogenic powdery mildew fungi. This pre-invasive depends on the formation ternary protein complexes consisting plasma membrane-localized PEN1 syntaxin, adaptor SNAP33 and closely sequence-related vesicle-resident VAMP721 or VAMP722 proteins. The thaliana resistance 8.2 (RPW8.2) confers disease mildews upon fungal...
Abstract CRISPR‐Cas9 system is now widely used to edit a target genome in animals and plants. Cas9 protein derived from Streptococcus pyogenes (SpCas9) cleaves double‐stranded DNA targeted by chimeric single‐guide RNA (sgRNA). For plant editing, Agrobacterium ‐mediated T‐DNA transformation has been broadly express proteins sgRNAs under the control of CaMV 35S U6/U3 promoter, respectively. We here developed simple high‐throughput binary vector clone 19−20 bp sgRNA, which binds reverse...
Actin filaments are thought to play an important role in intracellular trafficking various eukaryotic cells. However, their involvement plant cells has not been clearly demonstrated. Here, we investigated the roles actin using latrunculin B (Lat B), inhibitor of filament assembly, or mutants that disrupt when overexpressed. Lat and actin2 mutant overexpression inhibited two vacuolar reporter proteins, sporamin:green fluorescent protein (GFP) Arabidopsis thaliana aleurain-like protein:GFP,...
Genome sizes vary by several orders of magnitude, driven mechanisms such as illegitimate recombination and transposable element proliferation. Prior analysis the CesA region in two cotton genomes that diverged 5-10 million years ago (Ma), acquired a twofold difference genome size, revealed extensive local conservation genic intergenic regions, with no evidence global size difference. The present study extends comparison to include BAC sequences surrounding gene encoding alcohol dehydrogenase...