A5085701434- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Endoplasmic Reticulum Stress and Disease
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Reproductive Biology and Fertility
- Calcium signaling and nucleotide metabolism
- Lipid metabolism and biosynthesis
- Plant and animal studies
- RNA modifications and cancer
- PI3K/AKT/mTOR signaling in cancer
- Plant nutrient uptake and metabolism
- RNA Interference and Gene Delivery
- Lysosomal Storage Disorders Research
- Chromosomal and Genetic Variations
- Sexual Differentiation and Disorders
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Legume Nitrogen Fixing Symbiosis
- Estrogen and related hormone effects
The University of Tokyo
2000-2024
Roswell Park Comprehensive Cancer Center
2023
Kyoto University
2022-2023
Kansai Medical University
2022
Nagoya City University
2013-2021
Temple University
2019
Osaka Nishi Clinic
2018
RIKEN Center for Advanced Photonics
2017
Center for Children
2017
Tokyo University of Science
1998-2016
A ferritin particle consists of 24 proteins (FTH1 and FTL) stores iron ions within it. During deficiency, particles are transported to lysosomes release ions. Two transport pathways have been reported: macroautophagy ESCRT-dependent endosomal microautophagy. Although the membrane dynamics these differ, both require NCOA4, which is thought be an autophagy receptor for ferritin. However, it unclear whether NCOA4 only acts as in degradation. Here, we found that form liquid-like condensates a...
Leucine-rich repeat kinase 2 (LRRK2), a Rab associated with Parkinson’s disease and several inflammatory diseases, has been shown to localize stressed lysosomes get activated regulate lysosomal homeostasis. However, the mechanisms of LRRK2 recruitment activation have not well understood. Here, we found that ATG8 conjugation system regulates as LC3 onto single membranes lysosomes/phagosomes. This did require FIP200-containing autophagy initiation complex, nor it occur on double-membrane...
Summary The plant vacuole is a multifunctional organelle which essential for growth and development. To visualize the dynamics of vacuolar membranes, γ‐TIP (tonoplast intrinsic protein) was fused to GFP expressed in Arabidopsis thaliana . marker molecule targeted membranes most tissues, as expected. In rapidly expanding cells, some additional spherical structures were often observed within lumen vacuoles, emitted strong fluorescence. confirm their normal presence, we examined wild‐type...
In all eukaryotic cells, a membrane-trafficking system connects the post-Golgi organelles, such as trans-Golgi network (TGN), endosomes, vacuoles, and plasma membrane. This complex plays critical roles in several higher-order functions multicellular organisms. The TGN, one of important organelles for protein transport network, sorting station, where cargo proteins are directed to appropriate compartments. Unlike its animal yeast TGN has also been reported function like early endosomal...
Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, morphogenesis and organelle motility. Imaging the actin cytoskeleton living cells is powerful methodology for studying these important phenomena. Several useful probes live imaging filamentous (F-actin) have been developed, but new versatile are still needed. Here, we report application probe called Lifeact visualizing F-actin cells. short peptide comprising 17 amino acids that...
Autophagy is an essential catabolic pathway which sequesters and engulfs cytosolic substrates via autophagosomes, unique double-membraned structures. ATG8 proteins are ubiquitin-like recruited to autophagosome membranes by lipidation at the C-terminus. ATG8s recruit substrates, such as p62, play important role in mediating membrane expansion. However, precise function of lipidated expansion remains obscure. Using a real-time vitro assay, we revealed that N-termini human (LC3B GABARAP) highly...
Summary Two similar Arabidopsis dynamin‐related proteins, DRP3A and DRP3B, are thought to be key factors in both mitochondrial peroxisomal fission. However, the functional genetic relationships between DRP3B have not been fully investigated. In a yeast two‐hybrid assay, interacted with themselves each other. localized mitochondria peroxisomes, co‐localized other leaf epidermal cells. two T‐DNA insertion mutants, drp3a drp3b , little longer fewer number than those wild‐type double mutant,...
Protein export from the endoplasmic reticulum (ER) to Golgi apparatus occurs at specialized regions known as ER exit sites (ERES). In Saccharomyces cerevisiae, ERES show numerous scattered puncta throughout ER. We examined localization within peripheral ER, finding that localize on high-curvature domains where curvature-stabilizing protein Rtn1 is present. Δrtn1 Δrtn2 Δyop1 cells have fewer domains, but accumulate remaining edge of expanded sheets. propose membrane curvature a key geometric...
Brassinazole (Brz) is a specific inhibitor of the biosynthesis brassinosteroids (BRs), which regulate plant organ and chloroplast development. We identified recessive pale green Arabidopsis mutant, bpg2-1 (Brz-insensitive-pale 2-1) that showed reduced sensitivity to chlorophyll accumulation promoted by Brz in light. BPG2 encodes chloroplast-localized protein with zinc finger motif four GTP-binding domains are necessary for normal biogenesis. BPG2-homologous genes evolutionally conserved...
SNAREs (soluble N-ethylmaleimide sensitive factor attachment protein receptors) mediate specific membrane fusion between transport vesicles or organelles and target membranes. VAM3/SYP22 PEP12/SYP21 are Qa-SNAREs that act in the vacuolar pathway of Arabidopsis thaliana, localized predominantly on pre-vacuolar compartment (PVC), respectively. Previous studies have shown loss-of-function mutants showed male gametophytic lethality, suggesting possess different, non-redundant functions. We...
In macroautophagy, disk-shaped double-membrane structures called phagophores elongate to form cup-shaped structures, becoming autophagosomes upon closure. These then fuse with lysosomes become autolysosomes and degrade engulfed material. Autophagosome formation is reported involve other organelles, including the endoplasmic reticulum (ER) mitochondria. Organelles are also taken up by as autophagy cargos. However, few studies have performed systematic spatiotemporal analysis of...
Abstract β-propellers that bind polyphosphoinositides (PROPPINs) are an autophagy-related protein family conserved throughout eukaryotes. The PROPPIN includes Atg18, Atg21 and Hsv2 in yeast WD-repeat interacting with phosphoinositides (WIPI)1–4 mammals. Mutations the WIPI genes associated human neuronal diseases, including β-propeller neurodegeneration (BPAN) caused by mutations WDR45 (encoding WIPI4). In contrast to PROPPINs, functions of mammalian WIPI1–WIPI4 have not been systematically...
The formation of autophagosomes involves dynamic morphological changes a phagophore from flat membrane cisterna into cup-shaped intermediate and spherical autophagosome. However, the physical mechanism behind these remains elusive. Here, we determine average shapes phagophores by statistically investigating three-dimensional electron micrographs more than 100 phagophores. results show that structures adopt characteristic morphology; they are longitudinally elongated, rim is catenoidal with...
The cDNA clone of NtSAR1, a gene encoding the small GTPase Sar1p which is essential for vesicle formation from endoplasmic reticulum (ER) membrane in yeast, has been isolated Nicotiana tabacum BY-2 cells. NtSAR1 as well AtSAR1 Arabidopsis thaliana [d'Enfert et al. (1992) EMBO J. 11: 4205] could complement lethality disruption SAR1 yeast cells temperature-sensitive fashion. They also suppressed sec12 and sec16 mutations does. Using this complementation system, we analyzed phenotypes several...
Abstract Arabidopsis thaliana zigzag (zig) is a loss-of-function mutant of Qb-SNARE VTI11, which involved in membrane trafficking between the trans-Golgi network and vacuole. zig-1 exhibits abnormalities shoot gravitropism morphology. Here, we report that mutants retromer large subunit partially suppress phenotype. Moreover, demonstrate three paralogous VPS35 genes have overlapping but distinct genetic functions with respect to suppression. Tissue-specific complementation experiments using...
Abstract The field of microbiology was established in the 17 th century upon discovery microorganisms by Antonie van Leeuwenhoek using a single-lens microscope. Now, detailed ultrastructures can be elucidated situ three-dimensional electron microscopy. Since availability microscopy, taxonomy microscopic organisms has entered new era. Here, we taxonomic system primitive algal genus Glaucocystis (Glaucophyta) new-generation methodology: ultra-high-voltage microscopy (UHVEM) and field-emission...