A5040876355- Cancer Cells and Metastasis
- Liver physiology and pathology
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Gastric Cancer Management and Outcomes
- Gastrointestinal Tumor Research and Treatment
- 3D Printing in Biomedical Research
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Galectins and Cancer Biology
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Pancreatic function and diabetes
- Cancer Research and Treatments
- Metastasis and carcinoma case studies
- Neuroblastoma Research and Treatments
- FOXO transcription factor regulation
- Analytical Chemistry and Chromatography
- Estrogen and related hormone effects
- Cellular Mechanics and Interactions
- Lung Cancer Research Studies
Osaka University
2011-2025
Kyoto University
2018-2025
University of Connecticut
2024
Biorasis (United States)
2024
Kyushu University
2024
Vanderbilt University Medical Center
2015-2023
Osaka International Cancer Institute
2017-2023
Kansai Medical University
2022
Vanderbilt University
2017
Osaka Medical Center for Cancer and Cardiovascular Diseases
2011-2016
Primary culture of the cancer cells from patients’ tumors can provide crucial information individual tumors, yet technology has not been optimized until now. We developed an innovative method for primary colorectal cells, based on principle that cell–cell contact was maintained throughout process. When tumor tissue dissociated into cell clusters, in which retained, they rapidly formed spheroids we termed tissue-originated (CTOSs). CTOSs consisted highly purified and viable were prepared with...
<h3>Objective</h3> MicroRNAs (miRNAs) act as tumour suppressor genes or oncogenes in the regulation of multiple carcinogenic processes. Aberrant miRNA expression is reported <i>Helicobacter pylori</i> (<i>H pylori</i>)-related gastritis and gastric cancer. The cytotoxin-associated gene A (CagA) <i>H has a pathophysiologically important role carcinogenesis. study was undertaken to evaluate effect CagA on its regulatory mechanism. <h3>Methods</h3> assessed by comprehensive microarray....
Patient‐derived cancer organoid culture is an important live material that reflects clinical heterogeneity. However, the limited amount of organoids available for each case as well considerable time and cost to expand in vitro makes it impractical perform high‐throughput drug screening using cultures from multiple patients. Here, we report advanced system 2427 drugs tissue‐originated spheroid ( CTOS ) method. In this system, apply method ex vivo platform xenograft tumors, machines handle...
Abstract In chronic liver diseases (CLD), p53 is constitutively activated in hepatocytes due to various etiologies as viral infection, ethanol exposure, or lipid accumulation. This study was aimed clarify the significance of activation on pathophysiology CLDs. Kras-mutant cancer model, murine double minute 2 (Mdm2), a negative regulator p53, specifically deleted [Alb-Cre KrasLSL-G12D Mdm2fl/fl (LiKM; KrasG12D mutation and Mdm2 loss liver)]. Accumulation upregulation its downstream genes were...
Heparin is given to patients undergoing colonoscopic polypectomy at high risk for thromboembolism. Little known, however, about how heparin bridge therapy (HB) affects post-polypectomy bleeding (PPB). The present study aimed identify the clinical features of PPB associated with HB.Data consecutive inpatients who underwent antithrombotic Osaka University Hospital were retrospectively collected and categorized into a HB group or non-HB group. incidence characteristics analyzed.A total 117 279...
WD repeat domain 5 (WDR5) is a member of the WD40-repeat protein family that plays critical role in multiple chromatin-centric processes. Overexpression WDR5 correlates with poor clinical outcome many human cancers, and itself has emerged as an attractive target for therapy. Most drug-discovery efforts center on WIN site responsible recruitment to chromatin. Here, we describe discovery novel antagonists containing dihydroisoquinolinone bicyclic core using structure-based design. These...
Dynamic changes in cell properties lead to intratumor heterogeneity; however, the mechanisms of nongenetic cellular plasticity remain elusive. When fate each from colorectal cancer organoids was tracked through a clonogenic growth assay, cells showed wide range ability even within clonal organoids, consisting distinct subpopulations; generating large spheroids and small spheroids. The generated only (S-pattern), while both (D-pattern), which were tumorigenic. Transition S-pattern D-pattern...
Photon upconversion (UC) from red or near-infrared (NIR) light to blue is promising for in vivo optogenetics. However, the examples of optogenetics have been limited lanthanide inorganic UC nanoparticles, and there no without using heavy metals. Here first example biocompatible metal-free TTA-UC nanoemulsions shown. A new organic TADF sensitizer, a boron difluoride curcuminoid derivative modified with bromo group, can promote intersystem crossing excited triplet state, significantly...
Spread through air spaces (STAS) is a histological finding of lung tumours where tumour cells exist within the space parenchyma beyond margin main tumour. Although STAS an important prognostic factor, pathobiology remains unclear. Here, we investigated mechanism by analysing relationship between and polarity switching in vivo vitro. Histopathological analysis revealed that apical membranes were observed outside lesions around colorectal cancer (CRC) metastases adenocarcinomas. When...
The poor prognosis of pancreatic cancer is often attributed to difficulties early detection due a lack appropriate risk factors. Previously, we demonstrated the presence Enterococcus faecalis (E. faecalis) in juice and tissues obtained from patients with cancers duodeno-pancreato-biliary region, suggesting possible involvement this bacterial species chronic malignant diseases. However, it remains unclear if how E. can infect ductal cells. In study, used immortalized normal human epithelial...
LRIG1, a membrane glycoprotein, has emerged as significant stem cell marker and negative regulator of receptor tyrosine kinases (RTKs), including EGFR. Glycosylation is major post-translational modification, which plays crucial role in protein function stability. In cancer biology, abnormal glycosylation can serve biomarker contribute to pathogenesis. We aimed investigate the effects on LRIG1 functions. Through database analysis experimental approaches, we focused evolutionarily conserved...
Cancer-associated fibroblasts (CAFs) create a microenvironment that contributes to tumor growth; however, the mechanism by which are phenotypically altered CAFs remains unclear. Loss or mutation of suppressor p53 plays crucial role in cancer progression. Herein, we analyzed how status cells affects investigating vivo and vitro effects loss function on phenotypic changes subsequent progression human colon cell lines containing wild-type with functional deficiency. The growth p53-deficient...
Recent studies have demonstrated that cancer stem cells (CSCs) can initiate and sustain tumor growth exhibit resistance to clinical cytotoxic therapies. Therefore, CSCs represent the main target of anticancer therapy. Interleukin-6 (IL-6) promotes cellular proliferation drug in colorectal cancer, its serum levels correlate with patient survival. IL-6 downstream signaling molecule signal transducer activator transcription-3 (STAT3) potential molecular targets. In present study, we...
Flow of fluids through the gut, such as milk from a neonatal diet, generates shear stress on unilaminar epithelium lining lumen. We report that exposure to physiological levels fluid leads formation large vacuoles, containing extracellular contents within polarizing intestinal epithelial cell monolayers. These observations lead two questions: how can cells lacking primary cilia transduce stress, and what molecular pathways support vacuoles exceed 80% volume? find forces are sensed by...
Abstract Prostate-specific antigen (PSA) is the most frequently used biomarker for screening of prostate cancer. Understanding structure cancer-specific glycans can help us improve PSA assay. In present study, we analysed obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that parent tumour to explore new candidates cancer-related glycoforms PSA. The glycan profile CTOS was determined by comparing with normal seminal plasma...
Background and Aims: Intrahepatic cholangiocarcinoma (ICC) is a deadly but poorly understood disease, its treatment options are very limited. The aim of this study was to identify the molecular drivers ICC search for therapeutic targets. Approach Results: We performed Sleeping Beauty transposon‐based in vivo insertional mutagenesis screen liver‐specific Pten ‐deficient mice identified TNF receptor–related factor 3 ( Traf3 ) as most significantly mutated gene murine ICCs loss‐of‐function...
We evaluated whether endoscopic ultrasonography (EUS) image quality affects the accuracy of diagnosing vertical invasion depth early gastric cancer (EGC). A total 75 lesions in patients suspected having EGC were enrolled. All underwent EUS examination. Findings compared with histopathologic results. effect following clinicopathologic factors: location, diameter, surface pattern, concomitant ulceration, histology type, and score. was scored based on detection repeatability, appropriate probe...