A5061735306- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- 3D Printing in Biomedical Research
- Neuroblastoma Research and Treatments
- Renal cell carcinoma treatment
- FOXO transcription factor regulation
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- TGF-β signaling in diseases
- Reproductive Biology and Fertility
- Bone Tissue Engineering Materials
- Adipokines, Inflammation, and Metabolic Diseases
- Lung Cancer Research Studies
- Collagen: Extraction and Characterization
- Neuroendocrine Tumor Research Advances
- Liver physiology and pathology
- Porphyrin and Phthalocyanine Chemistry
- Digestive system and related health
- Bone and Dental Protein Studies
- PI3K/AKT/mTOR signaling in cancer
- Ovarian cancer diagnosis and treatment
Kyoto University
2020-2025
Tottori University
2014-2023
Kansai Medical University
2022
Japan Society for the Promotion of Science
2009
Yamagata University
2006-2009
Reactive oxygen species are involved in the aging process and diseases. Despite important role of Cu/Zn SOD (superoxide dismutase) encoded by SOD1, SOD1-/- mice appear to grow normally under conventional breeding conditions. In present paper we report on a novel finding showing distinct connection between oxidative stress erythrocytes production autoantibodies against mice. Evidence is presented show that SOD1 primarily required for maintaining erythrocyte lifespan suppressing stress. A...
Animal models for human colorectal cancer recapitulate multistep carcinogenesis that is typically initiated by activation of the Wnt pathway. Although potential roles both genetic and environmental modifiers have been extensively investigated in vivo, it remains elusive whether epithelial cells definitely require interaction with stromal or microflora tumor development. Here we show development could be simply induced independently intestinal microenvironment, even WT murine primary alone....
The cystine/glutamate antiporter, system xc- , is essential for the efficient uptake of cystine into cells. Interest in mechanisms function soared with recognition that presents most upstream node ferroptosis, a recently described form regulated necrosis relevant degenerative diseases and cancer. Since targeting hold great potential to efficiently combat tumor growth metastasis certain tumors, we disrupted substrate-specific subunit xCT (SLC7A11) highly metastatic mouse B16F10 melanoma cell...
Abstract DNA methyltransferases (DNMTs) catalyze methylation at the C5 position of cytosine with S -adenosyl- l -methionine. Methylation regulates gene expression, serving a variety physiological and pathophysiological roles. The chemical mechanisms regulating DNMT enzymatic activity, however, are not fully elucidated. Here, we show that protein S-nitrosylation cysteine residue in DNMT3B attenuates activity consequent aberrant upregulation expression. These genes include Cyclin D2 ( Ccnd2 ),...
Dynamic changes in cell properties lead to intratumor heterogeneity; however, the mechanisms of nongenetic cellular plasticity remain elusive. When fate each from colorectal cancer organoids was tracked through a clonogenic growth assay, cells showed wide range ability even within clonal organoids, consisting distinct subpopulations; generating large spheroids and small spheroids. The generated only (S-pattern), while both (D-pattern), which were tumorigenic. Transition S-pattern D-pattern...
Aim: To generate sunitinib-resistant renal cell carcinoma (RCC) lines and identify miRNAs contributing to sunitinib resistance. Materials Methods: Two RCC lines, ACHN RCC23, were cultured by continuous treatment with for 3 months, doses gradually increasing up the 50% inhibitory concentration each line. We performed microarray quantitative real-time polymerase chain reaction analyses of (SR-ACHN) RCC23 (SR-RCC23) cells, as well sunitinib-sensitive cells. Results: SR-ACHN SR-RCC23 cells...
Since liver metastasis is the main cause of death in cancer patients, we attempted to identify driver gene involved. QRsP-11 fibrosarcoma cells were injected into spleens syngeneic mice isolate tumour sub-populations that colonize liver. Cells from metastatic nodules established and subsequently intrasplenically for selection. After 12 cycles, cell subline LV12 was obtained. Intravenous injection produced more metastases than cells, whereas incidence lung similar cells. adhered liver-derived...
We have established an inflammation-related carcinogenesis model in mouse, which regressive QR-32 cells subcutaneously co-implanted with a foreign body—gelatin sponge—convert themselves into lethal tumors due to massive infiltration of inflammatory the sponge. Animals were fed diet containing 5% or 10% fermented brown rice and bran Aspergillus oryzae (FBRA). In FBRA groups, tumor incidences lower (35% 20%, respectively) than non-treated group (70%). found that reduced number infiltrating...
Abstract Background The onset mechanism for bisphosphonate‐related osteonecrosis of the jaw (BRONJ) has been reported, with a focus on bone remodeling, biofilm formation, and epithelial cell proliferation migration. However, involvement stromal cells, especially fibroblasts, in oral cavity is unclear. Therefore, this study was focused how bisphosphonates (BPs) affect orthotopic periodontal ligament fibroblasts from viewpoint oxidative stress compared ectopically obtained fibroblasts. Methods...
Spread through air spaces (STAS) is a histological finding of lung tumours where tumour cells exist within the space parenchyma beyond margin main tumour. Although STAS an important prognostic factor, pathobiology remains unclear. Here, we investigated mechanism by analysing relationship between and polarity switching in vivo vitro. Histopathological analysis revealed that apical membranes were observed outside lesions around colorectal cancer (CRC) metastases adenocarcinomas. When...
Abstract Collagen is the most abundant protein in human body and one of main components stromal tissues tumors which have a high elastic modulus over 50 kPa. Although collagen has been widely used as cell culture scaffold for cancer cells, there limitations when attempting to fabricate tough gel with cells like stroma. Here, rapid gelation solution within few minutes by transition metal complexation demonstrated. Type I at neutral pH shows transparency 81% 1,781 kPa mixing K 2 PtCl 4 3 min....
Reactive oxygen species (ROS) are likely candidates for involvement in ischemia/reperfusion-induced acute renal failure (ARF). In this study, the issue of whether superoxide dismutase (SOD1)-deficiency exacerbates ARF was examined. At two weeks after a right nephrectomy mice, left vessels were clipped to induce ischemia and then released 45 min. The severe damage observed at one day partially recovered seven days induction ischemia. SOD1-/- mice suffer from compared with SOD1+ - SOD1+/+...
Abstract The bone morphogenetic protein (BMP) pathway promotes differentiation and induces apoptosis in normal colorectal epithelial cells. However, its role cancer (CRC) is controversial, where it can act as context‐dependent tumor promoter or suppressor. Here we have found that CRC cells reside a BMP‐rich environment based on curation of two publicly available RNA‐sequencing databases. Suppression BMP using specific inhibitor, LDN193189, suppresses the growth select organoids. Colorectal...
Weakly tumorigenic and nonmetastatic QR-32 cells derived from a fibrosarcoma in C57BL6 mouse are converted to malignant once they have grown after being coimplanted with gelatine sponge which induces inflammation. We administered newly developed peroral superoxide dismutase (SOD), oxykine, as control vehicle, gliadin saline, starting 2 days before the coimplantation continued daily throughout experiment. In oxykine group, tumour incidence was lower (41%) than or saline group (83 79%,...
By a proteomics‐based approach, we identified an overexpression of fascin in colon adenocarcinoma cells ( FPCK pP‐3) that developed from nontumorigenic human colonic adenoma ‐1–1) and were converted to tumorigenic by foreign‐body‐induced chronic inflammation nude mice. Fascin was also observed the tumors arising rat intestinal epithelial IEC 6) which induced same manner. Upregulation expression ‐1–1 transfection with sense c DNA tumorigenic, whereas antisense fascin‐c ‐transfected pP‐3...
Abstract Micropapillary carcinoma (MPC) is a morphologically distinctive form of carcinoma, composed small nests cancer cells surrounded by lacunar spaces. Invasive MPC associated with poor prognosis. The tumor in reportedly exhibit reverse polarity, although the molecular mechanisms underlying patterns are poorly understood. Using tissue‐originated spheroid (CTOS) method, we previously reported polarity switching colorectal (CRC). When cultured suspension, apical membrane promptly switches...
Peritoneal dissemination is a predominant pattern of metastasis in patients with advanced ovarian cancer. Despite recent progress the management strategy, peritoneal remains determinant poor cancer prognosis. Using various histological types patient-derived organoids, roles apicobasal polarity cell clusters were studied. First, it was found that both tissues and organoids showed polarity, where zonula occludens-1 (ZO-1) integrin beta 4 (ITGB4) served as markers for apical basal sides,...