A5090020302- Malaria Research and Control
- Mosquito-borne diseases and control
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Stress Responses and Cortisol
- Complement system in diseases
- Immune Response and Inflammation
- Drug Transport and Resistance Mechanisms
- Computational Drug Discovery Methods
- Vitamin C and Antioxidants Research
- Bacterial Infections and Vaccines
- Bipolar Disorder and Treatment
- Neuroendocrine regulation and behavior
- Neuroscience and Neuropharmacology Research
- Heme Oxygenase-1 and Carbon Monoxide
- Pneumonia and Respiratory Infections
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Amino Acid Enzymes and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory mediators and NSAID effects
- Thyroid Disorders and Treatments
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- Gut microbiota and health
The University of Sydney
2014-2023
University of Oxford
2023
Rush University Medical Center
2022
University of Bristol
2018
Robert Bosch (Australia)
2007-2017
Hebrew University of Jerusalem
2013
Universität Hamburg
1984-2009
Pulmonary Vascular Research Institute
2007
Boehringer Ingelheim (Germany)
2004
Woolcock Institute of Medical Research
2004
We investigated the relationship between gut health, visceral fat dysfunction and metabolic disorders in diet-induced obesity. C57BL/6J mice were fed control or high saturated diet (HFD). Circulating glucose, insulin inflammatory markers measured. Proximal colon barrier function was assessed by measuring transepithelial resistance mRNA expression of tight-junction proteins. Gut microbiota profile determined 16S rDNA pyrosequencing. Tumor necrosis factor (TNF)-α interleukin (IL)-6 levels...
A rapid reduction in parasitemia associated with damage to intraerythrocytic parasites was observed Plasmodium vinckei-infected mice after they had received a single intravenous injection of alloxan. This not prevented by prior glucose, but desferrioxamine or diethyldithiocarbamate. Prior propanol partially blocked the phenomenon. transient hemolysis malaria-infected mice, controls, also desferrioxamine, glucose. Both fall and occurred, less dramatically, when phenylhydrazine hydrogen...
Objective To distinguish the effects of dietary fat profile on gut parameters and their relationships with metabolic changes to determine capacity n-3 fatty acids modify variables in context diet-induced dysfunctions. Methods Mice received control or high-fat diets emphasizing saturated (HFD-sat), n-6 (HFD-n6), (HFD-n3) for 8 weeks. In another cohort, mice that were maintained HFD-sat n-3-rich fish oil resolvin D1 supplementation. Results HFD-n6 induced similar weight gain, but only...
Cell-mediated immunity to malaria may involve macrophages, the monokines that mediate endotoxicity, and reactive oxygen species. Since interferon-gamma activates macrophages release species, tumor necrosis factor-alpha (TNF-alpha) helps both endotoxicity induce leukocytes secrete oxygen, we monitored effects of administering recombinant forms these cytokines on Plasmodium chabaudi adami infections in mice. We also fed infected mice a diet containing 0.75% butylated hydroxyanisole, scavenger...
Understanding biochemical mechanisms and changes associated with disease conditions and, therefore, development of improved clinical treatments, is relying increasingly on various mapping imaging techniques tissue sections. However, it essential to be able ascertain whether the sampling used provides full information relevant free from artefacts. A multi-modal micro-spectroscopic approach, including FTIR PIXE elemental mapping, has been study molecular profile within cryofixed formalin-fixed...
Abstract Cerebral malaria (CM) can be a fatal manifestation of Plasmodium falciparum infection. Using murine models malaria, we found much greater up-regulation number chemokine mRNAs, including those for CXCR3 and its ligands, in the brain during CM (FMCM) than model non-CM. Expression CXCL9 CXCL10 RNA was localized predominantly to cerebral microvessels adjacent glial cells, while expression CCL5 restricted mainly infiltrating lymphocytes. The majority mice deficient were protected from...
Expression of the relaxin-like factor (RLF) was studied at messenger RNA (mRNA) and protein levels in testes ovaries mouse, as well through testicular development differentiation mouse testis. In situ hybridization or RT-PCR, immunohistochemistry using a polyclonal antibody raised against recombinant protein, provided mutually confirmatory results for high expression RLF Leydig cells adult testis much lower level luteal ovary cycle, pregnancy, lactation. Analysis mRNA expression, postnatal...
SUMMARY CBA/T6 and Balb/c mice inoculated with Plasmodium berghei ANKA strain (PbA) died from cerebral malaria 6–8 days post-inoculation. DBA/2J similarly developed a non-fatal malaria, mild temporary symptoms, between 15 22 other malaria-related complications. When P. K173 (Pb) these mouse strains did not develop but These strain/parasite combinations allow for detailed examination of factors critical in the pathology murine malaria. Monastral Blue, colloid dye, when injected...
SUMMARY Cerebral malaria in A/J and CBA/H mice infected with Plasmodium berghei ANKA is accompanied by mononuclear cell infiltration, haemorrhage cerebral endothelial damage. This damage presumably one of the causes breakdown blood—brain barrier which was detected measuring movement dye Evans blue radioisotope labelled albumin erythrocytes. The density brain tissue, measured a Percoll gradient technique, significantly reduced exhibiting symptoms, suggesting occurrence oedema.
Abstract Because oxygen-centered free radicals are involved in cell-mediated immune responses, we examined the possibility that these reactive species could also have a role lymphoproliferative response to alloantigens mixed lymphocyte culture (MLC). Three classes of agents prevent formation, or damaging effects, oxygen were tested: non-permeant electron acceptor ferricyanide; iron chelators desferrioxamine, desferrithiocin, octanohydroxamic acid, and pyridoxal isonicotinoyl hydrazone, which...