A5043141098- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- Extracellular vesicles in disease
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Drug Transport and Resistance Mechanisms
- RNA Interference and Gene Delivery
- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Multiple Sclerosis Research Studies
- Viral Infections and Vectors
- Immune cells in cancer
- Sepsis Diagnosis and Treatment
- Parasites and Host Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Barrier Structure and Function Studies
- Computational Drug Discovery Methods
- Vector-borne infectious diseases
The University of Sydney
2016-2025
Taronga Conservation Society Australia
2017-2018
World Health Organization
1983-2015
University of Geneva
1990-2015
Robert Bosch (Australia)
2006-2015
Westmead Institute for Medical Research
2015
Hospital de Cruces
2002-2014
Centre National de la Recherche Scientifique
1998-2008
Institut de Neurobiologie de la Méditerranée
1999-2008
Johns Hopkins University
2008
To assess the diagnostic value of procalcitonin (PCT), interleukin (IL)-6, IL-8, and standard measurements in identifying critically ill patients with sepsis, we performed prospective 78 consecutive admitted acute systemic inflammatory response syndrome (SIRS) suspected infection. We estimated relevance different parameters by using multivariable regression modeling, likelihood-ratio tests, area under receiver operating characteristic curves (AUC). The final diagnosis was SIRS 18 patients,...
To investigate the role of tumor necrosis factor in Plasmodium falciparum infections, we measured serum concentrations this cytokine 65 Malawian children with severe malaria. Of these (mean age, 5.3 years), 55 were unconscious and 10 had hypoglycemia at presentation. Although there was considerable overlap, mean (±SEM) initial concentration significantly higher patients who died (709±312 pg per milliliter) than survived (184±32 milliliter; P<0.02). The mortality rate increased factor: a...
Tumor necrosis factor, or cachectin (TNF-α), a protein with wide range of biological activities, is produced mainly by macrophages and may be important in inflammatory processes. The role TNF-α the pathogenesis cerebral malaria was investigated murine model. Most CBA mice infected Plasmodium berghei anka die between days 6 14 acute neurological manifestations unrelated to level parasitemia, whereas some other strains have same severity that ends death after 3 4 weeks without manifestations....
Because of the possible involvement cytokines in gram-negative septicemia, we investigated serum levels tumor necrosis factor alpha, interleukin-1 beta, alpha interferon, and gamma interferon children with sepsis purpura fulminans. We studied 55 patients (ages, 1 month to 19 years) a clinical diagnosis purpuric lesions who were shock or had three more other biologic risk factors. The mortality rate was correlated number factors present on admission hospital (P = 0.03). Tumor elevated 91...
Microparticles (MPs) resulting from vesiculation of platelets and other blood cells have been extensively documented in vitro found increased numbers several vascular diseases, but little is known about MPs endothelial origin. The aim this study was to analyze morphological, immunological, functional characteristics derived human umbilical vein (HUVECs) stimulated by TNF, investigate whether these are detectable healthy individuals patients with a prothrombotic coagulation abnormality....
Lethally irradiated mice were injected with semiallogeneic, T-depleted bone marrow cells and an amount of peripheral T lymphocytes sufficient to induce graft-vs.-host disease (GVHD) becoming apparent on the second week after graft leading increasing mortality rate within following weeks (greater than 90% 80 d). Mice receiving alone had no GVHD used as controls. Beginning day 8, weekly 2 mg either rabbit anti-mouse recombinant tumor necrosis factor/cachectin (TNF-alpha) IgG, or normal IgG. On...
The role of TNF-alpha/cachectin in the pneumopathy elicited by bleomycin has been investigated. After a single intratracheal instillation, an increase lung TNF-alpha mRNA level was evident, from days 5 to 15, as shown Northern gel analysis whole RNA. In contrast, IL-1-alpha and GM-CSF were not detectable. mice passively immunized with rabbit anti-mouse IgG, bleomycin-induced collagen deposition, evaluated total hydroxyproline assay on day prevented. Depletion CD4 CD8 T lymphocytes vivo...
Serum concentrations of immunoreactive tumor necrosis factor/cachectin (TNF), interleukin-1 beta (IL-1 beta), interferon-gamma (IFN gamma), and interferon-alpha alpha) were prospectively measured in 70 patients with septic shock to determine their evolution prognostic values. In a univariate analysis, levels TNF (P = .002) IL-1 .05) associated the patient's outcome, but not IFN alpha .15) gamma .26). contrast, stepwise logistic regression severity underlying disease .01), age patient .02),...
Intrapulmonary activation of leukocytes and release cellular mediators enzymes are involved in the pathophysiology adult respiratory distress syndrome (ARDS). To investigate a possible role local cytokines, we measured bronchoalveolar fluid (BALF) plasma levels tumor necrosis factor (TNF-α) its soluble inhibitors (sTNF-RI + RII), interleukin-1β (IL-1β), interferon-α (IFN-α), granulocyte elastase 14 patients at risk for ARDS 35 developing after trauma, sepsis, or shock. During clinical...
In light of the recent controversies over role animal models for research into development new treatments severe malaria, particularly cerebral disease, a group scientists came together to discuss relative merits range and their overlap with complex clinical syndromes human disease.While it was not possible fully resolve differences utility Plasmodium berghei ANKA model experimental meeting did bring two communities closer identify further work provide information needed validate revitalise...
Interactions between glioma cells and their local environment are critical determinants of brain tumor growth, infiltration neovascularisation. Communication with host stroma via microvesicles represents one pathway by which tumors can modify surroundings to achieve a tumor-permissive environment. Here we have taken an unbiased approach identifying RNAs in glioma-derived microvesicles, explored potential regulate gene expression recipient cells. We find that predominantly exosomal origin...
We examined the role of cytokines in cutaneous response to application trinitrochlorobenzene (TNCB) both nonsensitized and sensitized mice, i.e., irritant reaction (IR) contact hypersensitivity reactions (CH). When administered immediately before challenge, anti-tumor necrosis factor (TNF) antibody abrogated ear swelling CH; directed against interferon gamma or antibodies granulocyte/macrophage colony-stimulating interleukin 3 (IL-3) had a partial inhibitory effect; anti-IL-2 receptor no...
The pathogenesis of fatal cerebral malaria (CM) is not well understood, in part because data from patients whom a clinical diagnosis was established prior to death are rare. In murine CM model, platelets accumulate brain microvasculature, and antiplatelet therapy can improve outcome. We determined whether also found vessels human CM, we performed immunohistopathology for platelet-specific glycoprotein, GPIIb-IIIa, on tissue multiple sites Malawian children whose illness severe malarial...
Experimental cerebral malaria (ECM), a lethal hyperacute neurological syndrome associated with high blood levels of tumor necrosis factor, develops in genetically susceptible (CBA/Ca) mice 7 days after infection Plasmodium berghei ANKA strain. Injections neutralizing monoclonal antibody against recombinant murine interferon gamma, not later than 4 infection, markedly reduced the incidence ECM and elevation serum factor. This treatment prevented lesions (plugging brain vessels by monocytes,...
In vivo injection of the hamster anti-murine CD3 monoclonal antibody 145 2C11 into BALB/c mice induces a massive systemic release several cytokines. Very high circulating levels tumor necrosis factor are detected both by enzyme-linked immunosorbent assay and L-929 bioassay 90 min following single 10 micrograms/mouse 2C11. Peak exclusively T cell-derived products such as interferon-gamma, interleukin 2 3 also to 8 h post-injection. Importantly, this cytokine is transient since none these...