A5001269400- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Cellular transport and secretion
- Evolution and Genetic Dynamics
- Cerebrovascular and genetic disorders
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Animal Virus Infections Studies
- Studies on Chitinases and Chitosanases
- Endoplasmic Reticulum Stress and Disease
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Machine Learning in Bioinformatics
- Virology and Viral Diseases
- IL-33, ST2, and ILC Pathways
- Galectins and Cancer Biology
- Cell Image Analysis Techniques
- Cell Adhesion Molecules Research
Institute of Genomics and Integrative Biology
2020-2024
Academy of Scientific and Innovative Research
2021-2024
Council of Scientific and Industrial Research
2024
Autophagy is an essential catabolic pathway which sequesters and engulfs cytosolic substrates via autophagosomes, unique double-membraned structures. ATG8 proteins are ubiquitin-like recruited to autophagosome membranes by lipidation at the C-terminus. ATG8s recruit substrates, such as p62, play important role in mediating membrane expansion. However, precise function of lipidated expansion remains obscure. Using a real-time vitro assay, we revealed that N-termini human (LC3B GABARAP) highly...
<ns4:p><ns4:bold>Background: </ns4:bold>India first detected SARS-CoV-2, causal agent of COVID-19 in late January 2020, imported from Wuhan, China. From March 2020 onwards, the importation cases countries rest world followed by seeding local transmission triggered further outbreaks India.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>We used ARTIC protocol-based tiling amplicon sequencing SARS-CoV-2 (n=104) different states India using a combination MinION and MinIT Oxford Nanopore Technology...
Lipophagy is a ubiquitous mechanism for degradation of lipid droplets (LDs) in lysosomes. Autophagy receptors selectively target organelles lysosomal degradation. The selective receptor lipophagy remains elusive. Using mouse liver phosphoproteomics and human transcriptomics, we identify vacuolar-protein-sorting-associated protein 4A (VPS4A), member large family AAA+ ATPases, as lipophagy. We show that phosphorylation VPS4A on Ser95,97 its localization to LDs response fasting drives...
The recent release of SARS-CoV-2 genomic data from several countries has provided clues into the potential antigenic drift coronavirus population. In particular, instability observed in spike protein necessitates immediate action and further exploration context viral-host interactions. By temporally tracking 527,988 genomes, we identified invariant hypervariable regions within protein. We evaluated combination mutations lineages found that maximum number lineage-defining were present...
Summary Autophagy is an essential catabolic pathway which sequesters and engulfs cytosolic substrates via autophagosomes, unique double-membraned structures. ATG8 proteins are ubiquitin-like recruited to autophagosome membranes by lipidation at the C-terminus. ATG8s recruit substrates, such as p62, play important role in mediating membrane expansion. However, precise function of lipidated expansion remains obscure. Using a real-time vitro assay, we revealed that N-termini human (LC3B...
Abstract The recent release of SARS-CoV-2 genomic data from several countries has provided clues into the potential antigenic drift coronavirus population. In particular, instability observed in spike protein necessitates immediate action and further exploration context viralhost interactions. By temporally tracking 527,988 genomes, we identified invariant hypervariable regions within protein. We evaluated combination mutations lineages found that maximum number lineage-defining were present...
Abstract Autophagy pathway plays a central role in cellular degradation. The proteins involved the core autophagy process are mostly localised on membranes or interact indirectly with lipid-associated proteins. Therefore, progress structure determination of ‘core proteins’ remained relatively limited. Recent paradigm shift structural biology that includes cutting-edge cryo-EM technology and robust AI-based Alphafold2 predicted models has significantly increased data points biology. Here, we...