Partha Rakshit
A5004877275- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- CRISPR and Genetic Engineering
- COVID-19 epidemiological studies
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Viral Infections and Immunology Research
- Vaccine Coverage and Hesitancy
- Hepatitis C virus research
- Antifungal resistance and susceptibility
- Biosensors and Analytical Detection
- Viral Infections and Outbreaks Research
- Nail Diseases and Treatments
- Virus-based gene therapy research
- Antimicrobial Resistance in Staphylococcus
- Tuberculosis Research and Epidemiology
- Hepatitis B Virus Studies
- Infection Control in Healthcare
- Plant Pathogenic Bacteria Studies
- Virology and Viral Diseases
- Plant Virus Research Studies
- RNA regulation and disease
- Infectious Diseases and Mycology
- Plant Pathogens and Fungal Diseases
National Center for Disease Control
2020-2024
MRC Laboratory of Molecular Biology
2021
Institute of Genomics and Integrative Biology
2021
Lady Hardinge Medical College
2013
Central Research Institute
2013
As the global severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic expands, genomic epidemiology and whole genome sequencing are being used to investigate its transmission evolution. Against backdrop of emergence "variants concern" (VOCs) during December 2020 an upsurge in a state western part India since January 2021, analysis spike protein mutations using sequence structural approaches were undertaken identify possible new variants gauge fitness current circulating...
Delhi, the national capital of India, experienced multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreaks in 2020 and reached population seropositivity >50% by 2021. During April 2021, city became overwhelmed COVID-19 cases fatalities, as a new variant, B.1.617.2 (Delta), replaced B.1.1.7 (Alpha). A Bayesian model explains growth advantage Delta through combination increased transmissibility reduced sensitivity to immune responses generated against earlier variants...
Abstract As the global severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic expands, genomic epidemiology and whole genome sequencing are being constantly used to investigate its transmissions evolution. In backdrop of emergence “variants concern” (VOCs) during December 2020 an upsurge in a state western part India since January 2021, analysis spike protein mutations using sequence structural approaches was undertaken identify possible new variants gauge fitness current...
The SARS-CoV-2 B.1.617 variant emerged in the Indian state of Maharashtra late 2020. There have been fears that 2 key mutations seen receptor-binding domain, L452R and E484Q, would additive effects on evasion neutralizing antibodies. We report spike bearing E484Q confers modestly reduced sensitivity to BNT162b2 mRNA vaccine-elicited antibodies following either first or second dose. effect is similar magnitude loss conferred by alone. These data demonstrate but lack synergistic sensitivity.
Abstract The SARS-CoV-2 B.1.617.2 (Delta) variant was first identified in the state of Maharashtra late 2020 and spread throughout India, outcompeting pre-existing lineages including B.1.617.1 (Kappa) B.1.1.7 (Alpha). In vitro , is 6-fold less sensitive to serum neutralising antibodies from recovered individuals, 8-fold vaccine-elicited as compared wild type Wuhan-1 bearing D614G. Serum titres against were lower ChAdOx-1 versus BNT162b2 vaccinees. spike pseudotyped viruses exhibited...
<title>Abstract</title> The SARS-CoV-2 B.1.617.2 (Delta) variant was first identified in the state of Maharashtra late 2020 and has spread throughout India, displacing B.1.1.7 (Alpha) other pre-existing lineages. Mathematical modelling indicates that growth advantage is most likely explained by a combination increased transmissibility immune evasion. Indeed vitro, delta less sensitive to neutralising antibodies sera from recovered individuals, with higher replication efficiency as compared...
Abstract Delhi, the national capital of India, has experienced multiple SARS-CoV-2 outbreaks in 2020 and reached a population seropositivity over 50% by 2021. During April 2021, city became overwhelmed COVID-19 cases fatalities, as new variant B.1.617.2 (Delta) replaced B.1.1.7 (Alpha). A Bayesian model explains growth advantage Delta through combination increased transmissibility partial reduction immunity elicited prior infection (median estimates; ×1.5-fold, 20% reduction). Seropositivity...
In the last few months, there has been a global catastrophic outbreak of severe acute respiratory syndrome disease caused by novel coronavirus SARS-CoV-2 affecting millions people worldwide. Early diagnosis and isolation are key to contain rapid spread virus. Towards this goal, we report simple, sensitive method detect virus using targeted mass spectrometric approach, which can directly presence from naso-oropharyngeal swabs. Using multiple reaction monitoring two peptides specific in 2.3...
<ns4:p><ns4:bold>Background: </ns4:bold>India first detected SARS-CoV-2, causal agent of COVID-19 in late January 2020, imported from Wuhan, China. From March 2020 onwards, the importation cases countries rest world followed by seeding local transmission triggered further outbreaks India.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>We used ARTIC protocol-based tiling amplicon sequencing SARS-CoV-2 (n=104) different states India using a combination MinION and MinIT Oxford Nanopore Technology...
Abstract During the course of COVID-19 pandemic, large-scale genome sequencing SARS-CoV-2 has been useful in tracking its spread and identifying variants concern (VOC). Viral host factors could contribute to variability within a that can be captured next-generation reads as intra-host single nucleotide variations (iSNVs). Analysing 1347 samples collected till June 2020, we recorded 16 410 iSNV sites throughout genome. We found ∼42% reported SNVs by 30 September 2020 consensus sequences...
Abstract Breakthrough infections with SARS-CoV-2 Delta variant have been reported in doubly-vaccinated recipients and as re-infections. Studies of viral spread within hospital settings highlighted the potential for transmission between patients health care workers benefits high-grade respiratory protection workers. However extent to which vaccination is preventative less well studied. Here, we analysed data from 118 vaccinated (HCW) across two hospitals India, constructing probable networks...
Abstract In the last few months, there has been a global catastrophic outbreak of severe acute respiratory syndrome disease caused by novel corona virus SARS-CoV-2 affecting millions people worldwide. Early diagnosis and isolation is key to contain rapid spread virus. Towards this goal, we report simple, sensitive method detect using targeted mass spectrometric approach, which can directly presence from naso-oropharyngeal swabs. Using multiple reaction monitoring two peptides specific in 2.3...
SUMMARY India first detected SARS-CoV-2, causal agent of COVID-19 in late January-2020, imported from Wuhan, China. March-2020 onwards; importation cases rest the countries followed by seeding local transmission triggered further outbreaks India. We used ARTIC protocol based tiling amplicon sequencing SARS-CoV-2 (n=104) different states using a combination MinION and MinIT Oxford Nanopore Technology to understand introduction transmission. The analyses revealed multiple introductions Europe...
ABSTRACT During the course of COVID-19 pandemic, large-scale genome sequencing SARS-CoV-2 has been useful in tracking its spread and identifying Variants Of Concern (VOC). Besides, viral host factors could contribute to variability within a that can be captured next-generation reads as intra-host Single Nucleotide Variations (iSNVs). Analysing 1, 347 samples collected till June 2020, we recorded 18, 146 iSNV sites throughout genome. Both, mutations RdRp well APOBEC ADAR mediated RNA editing...
ABSTRACT Background and objective During past decades, fungal rhinosinusitis (FRS) has been diagnosed more frequently. Knowing the flora its prevalence in chronic (CRS) patients will allow a better understanding of this disease, diagnosis treatment. We studied fungus CRS Delhi-NCR (National Capital Region) tertiary care hospital. Materials methods In prospective study, total 180 having were enrolled which functional endoscopic sinus surgery was done. 173 (96.1%) patients, polyps/tissue/mass...
Abstract Breakthrough infections with SARS-CoV-2 Delta variant have been reported in vaccine recipients and individuals infected previous variants. Studies of viral spread within hospital settings highlighted the potential for transmission between patients health care workers, benefits high-grade respiratory protection workers. However extent to which vaccination is preventative less well studies. We here analyse data from workers two hospitals India, constructing probable networks...
Abstract Breakthrough infections with SARS-CoV-2 Delta variant have been reported in vaccine recipients and individuals infected previous variants. However the potential for fully vaccinated (two doses) to transmit is unclear. We here analyse data from health care workers two hospitals India, constructing probable transmission networks epidemiological virus genome sequence using a suite of computational approaches. Among known cases we identify high probability that doubly transmitted...