A5002425964- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Virology and Viral Diseases
- Salmonella and Campylobacter epidemiology
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Intracerebral and Subarachnoid Hemorrhage Research
- Virus-based gene therapy research
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- Molecular Junctions and Nanostructures
- Acute Ischemic Stroke Management
- vaccines and immunoinformatics approaches
- Analytical Chemistry and Sensors
- Bone Tissue Engineering Materials
- Brucella: diagnosis, epidemiology, treatment
- Advanced Proteomics Techniques and Applications
- Mycobacterium research and diagnosis
- Mosquito-borne diseases and control
- Chronic Kidney Disease and Diabetes
- Pickering emulsions and particle stabilization
- Antimicrobial Peptides and Activities
Institute of Genomics and Integrative Biology
2020-2025
Academy of Scientific and Innovative Research
2020-2025
Council of Scientific and Industrial Research
2021-2025
Indian Veterinary Research Institute
2012-2024
Graphic Era University
2022-2024
Virginia Tech
2024
Discovery Institute
2024
Indian Council of Agricultural Research
2023
Shiv Nadar University
2023
Mahatma Gandhi Mission's Dental College and Hospital
2023
Heavy metal toxicity in water is a serious problem that may have harmful effects on human health and the ecosystem. Lead [Pb(II)] cadmium [Cd(II)] are two such heavy ions, present water, whose severity well-known well-studied. In current research, magnetic biochar composite (MBC) studied as an adsorbent material for effective removal of lead ions from solutions. Magnetite (Fe3O4) nanoparticles pine-needle-derived ultrasonicated were used different weight ratios to prepare APTES...
Abstract Introduction A single center open label phase II randomised control trial was done to assess the pathogen and host-intrinsic factors influencing clinical immunological benefits of passive immunization using convalescent plasma therapy (CPT), in addition standard care (SOC) severe COVID-19 patients, as compared patients only on SOC therapy. Methods Convalescent collected from recovered following a screening protocol which also included measuring anti SARS-CoV2 spike IgG content....
In the last few months, there has been a global catastrophic outbreak of severe acute respiratory syndrome disease caused by novel coronavirus SARS-CoV-2 affecting millions people worldwide. Early diagnosis and isolation are key to contain rapid spread virus. Towards this goal, we report simple, sensitive method detect virus using targeted mass spectrometric approach, which can directly presence from naso-oropharyngeal swabs. Using multiple reaction monitoring two peptides specific in 2.3...
A single center open label phase 2 randomised control trial (Clinical Trial Registry of India No. CTRI/2020/05/025209) was done to assess clinical and immunological benefits passive immunization using convalescent plasma therapy. At the Infectious Diseases Beleghata General Hospital in Kolkata, India, 80 patients hospitalized with severe COVID-19 disease fulfilling inclusion criteria (aged more than 18 years, either mild ARDS having PaO2/FiO2 200-300 or moderate 100-200, not on mechanical...
Macrophages release soluble mediators following efferocytic clearance of apoptotic cells to facilitate intercellular communication and promote the resolution inflammation. However, whether inflammation is modulated by extracellular vesicles (EVs) vesicular released efferocytes not known. We report that efferocyte-derived EVs express prosaposin, which binds macrophage GPR37 increase expression efferocytosis receptor Tim4 via an ERK-AP1-dependent signaling axis, leading increased efficiency...
Early prognostication of patient outcomes in intracerebral hemorrhage (ICH) is critical for care. We aim to investigate protein biomarkers’ role prognosticating ICH patients. assessed 22 biomarkers using targeted proteomics serum samples obtained from the dataset (N = 150). defined poor as modified Rankin scale score 3–6. incorporated clinical variables and regression models random forest-based machine learning algorithms predict mortality. report Odds Ratio (OR) or Hazard (HR) with 95%...
Clozapine, a poorly tolerated antipsychotic drug, is widely recognized as the only efficacious option in treatment-resistant psychosis. The United Kingdom (U.K.) National Institute of Clinical Excellence (NICE) guidance for its consideration defined threshold treatment resistance substantially more liberal than that utilized seminal studies efficacy. This study documented adherence to NICE patient group likely be enriched resistance: 150 consecutive assertive outreach and former...
Nuclear-retained long non-coding RNAs (lncRNAs) including MALAT1 have emerged as critical regulators of many molecular processes transcription, alternative splicing and chromatin organization. Here, we report the presence three conserved thermodynamically stable RNA G-quadruplexes (rG4s) located in 3' region MALAT1. Using rG4 domain-specific pull-down followed by mass spectrometry immunoprecipitation, demonstrated that structures are specifically bound two nucleolar proteins, Nucleolin (NCL)...
Ex vivo cellular system that accurately replicates sickle cell disease and β-thalassemia characteristics is a highly sought-after goal in the field of erythroid biology. In this study, we present generation progenitor lines with mutation using CRISPR/Cas9. The models exhibit similar differentiation profiles, globin expression proteome dynamics as patient-derived hematopoietic stem/progenitor cells. Additionally, these recapitulate pathological conditions associated both diseases. Hydroxyurea...
In the tumor microenvironment (TME), regulatory T cells (T regs ) adapt their metabolism to thrive in low-glucose, high-lactate conditions, but mechanisms remain unclear. Our study identifies CD38 as a key regulator of this adaptation by depleting nicotinamide adenine dinucleotide (oxidized form) (NAD + ), redirecting lactate-derived pyruvate toward phosphoenolpyruvate and bypassing tricarboxylic acid (TCA) cycle. This prevents accumulation α-ketoglutarate, which destabilizes inducing...
Abstract: In the field of regenerative medicine, numerous potential applications mesenchymal stem cells (MSCs) can be envisaged, due to their ability differentiate into a range tissues on basis substrate which they grow. With advances in nanotechnology, carbon nanotubes (CNTs) have been widely explored for use as cell culture tissue engineering applications. this study, canine bone marrow-derived MSCs were considered cellular model an vitro study elucidate collective processes, using three...
To understand the spread of SARS-CoV2, in August and September 2020, Council Scientific Industrial Research (India) conducted a serosurvey across its constituent laboratories centers India. Of 10,427 volunteers, 1058 (10.14%) tested positive for SARS-CoV2 anti-nucleocapsid (anti-NC) antibodies, 95% which had surrogate neutralization activity. Three-fourth these recalled no symptoms. Repeat serology tests at 3 (n = 607) 6 175) months showed stable anti-NC antibodies but declining Local...
MALAT1, an abundant lncRNA specifically localized to nuclear speckles, regulates alternative-splicing (AS). The molecular basis of its role in AS remains poorly understood. Here, we report three conserved, thermodynamically stable, parallel RNA-G-quadruplexes (rG4s) present the 3' region MALAT1 which this function. Using rG4 domain-specific RNA-pull-down followed by mass-spectrometry, RNA-immuno-precipitation, and imaging, demonstrate dependent localization Nucleolin (NCL) Nucleophosmin...
Lipid compositions of cells, tissues, and bio-fluids are complex, with varying concentrations structural diversity making their identification challenging. Newer methods for comprehensive analysis lipids thus necessary. Herein, we propose a targeted-mass spectrometry based lipidomics screening method using combination variable retention time window relative dwell weightage. Using this method, identified more than 1000 lipid species within 24-min. The limit detection varied from the...