A5019092763- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Adenosine and Purinergic Signaling
- T-cell and B-cell Immunology
- Cancer Mechanisms and Therapy
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- Phagocytosis and Immune Regulation
- Endoplasmic Reticulum Stress and Disease
- Drug Transport and Resistance Mechanisms
- melanin and skin pigmentation
- SARS-CoV-2 and COVID-19 Research
- Calcium signaling and nucleotide metabolism
- Trace Elements in Health
- Metal complexes synthesis and properties
- Immunodeficiency and Autoimmune Disorders
- Long-Term Effects of COVID-19
- Heme Oxygenase-1 and Carbon Monoxide
- Sphingolipid Metabolism and Signaling
- Quinazolinone synthesis and applications
Indian Institute of Chemical Biology
2019-2025
Academy of Scientific and Innovative Research
2022-2025
Homi Bhabha National Institute
2025
Raja Ramanna Centre for Advanced Technology
2025
University of South Carolina
2016-2024
Council of Scientific and Industrial Research
2021-2024
Medical University of South Carolina
2013-2023
MUSC Hollings Cancer Center
2014-2019
College of Charleston
2015
Chittaranjan National Cancer Institute
1980-2013
Alloreactive donor T cells are the driving force in induction of graft-versus-host disease (GVHD), yet little is known about cell metabolism response to alloantigens after hematopoietic transplantation (HCT). Here, we have demonstrated that undergo metabolic reprograming allogeneic HCT. Specifically, employed a murine BM transplant model and determined switch from fatty acid β-oxidation (FAO) pyruvate oxidation via tricarboxylic (TCA) cycle aerobic glycolysis, thereby increasing dependence...
Sphingosine 1-phosphate (S1P), a bioactive lysophospholipid generated by sphingosine kinase 1 (SphK1), regulates lymphocyte egress into circulation via S1P receptor (S1PR1) signaling, and it controls the differentiation of regulatory T cells (Tregs) helper-17 cells. However, mechanisms which receptor-independent SphK1-mediated intracellular levels modulate cell functionality remains unknown. We show here that SphK1-deficient maintain central memory phenotype exhibit higher mitochondrial...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing disease 2019 (COVID-19), has led to significant morbidity and mortality. While most suffer from mild symptoms, some patients progress severe with distress (ARDS) associated systemic hyperinflammation. Methods First, characterize key cytokines their dynamics in this hyperinflammatory condition, we assessed abundance correlative expression of a panel 48 progressing ARDS as compared disease. Then, an...
PD1 blockade therapy, harnessing the cytotoxic potential of CD8
Abstract Introduction A single center open label phase II randomised control trial was done to assess the pathogen and host-intrinsic factors influencing clinical immunological benefits of passive immunization using convalescent plasma therapy (CPT), in addition standard care (SOC) severe COVID-19 patients, as compared patients only on SOC therapy. Methods Convalescent collected from recovered following a screening protocol which also included measuring anti SARS-CoV2 spike IgG content....
A single center open label phase 2 randomised control trial (Clinical Trial Registry of India No. CTRI/2020/05/025209) was done to assess clinical and immunological benefits passive immunization using convalescent plasma therapy. At the Infectious Diseases Beleghata General Hospital in Kolkata, India, 80 patients hospitalized with severe COVID-19 disease fulfilling inclusion criteria (aged more than 18 years, either mild ARDS having PaO2/FiO2 200-300 or moderate 100-200, not on mechanical...
Mitochondria and endoplasmic reticulum (ER) share structural functional networks activate well-orchestrated signaling processes to shape cells' fate function. While persistent ER stress (ERS) response leads mitochondrial collapse, moderate ERS promotes Strategies boost antitumor T-cell function by targeting ER-mitochondria cross-talk have not yet been exploited. Here, we used carbon monoxide (CO), a short-lived gaseous molecule, test whether engaging conditions can improve functions in T...
Abstract Effector CD8+ T cells rely primarily on glucose metabolism to meet their biosynthetic and functional needs. However, nutritional limitations in the tumor microenvironment can cause T-cell hyporesponsiveness. Therefore, must acquire metabolic traits enabling sustained effector function at site elicit a robust antitumor immune response. Here, we report that IL12-stimulated have elevated intracellular acetyl CoA levels maintain IFNγ nutrient-deprived, tumor-conditioned media (TCM)....
Adoptive T-cell therapy (ACT) of cancer, which involves the infusion ex vivo-engineered tumor epitope reactive autologous T cells into tumor-bearing host, is a potential treatment modality for cancer. However, durable antitumor response following ACT hampered either by loss effector function or survival cells. Therefore, strategies to improve persistence and sustain are immense importance. Given role metabolism in determining therapeutic efficacy cells, we hypothesize that inhibition PIM...
T cells of the helper (Th)17 subset offer promise in adoptive T-cell therapy for cancer. However, current protocols ex vivo programming Th17 cells, which include TGFβ exposure, increase expression CD39 and CD73, two cell surface ATP ectonucleotidases that reduce effector functions promote immunosuppression. Here, we report ATP-mediated suppression IFNγ production by can be overcome genetic ablation CD73 or using IL1β instead to program vivo. cultured were also highly polyfunctional,...
Abstract Background In search of a suitable GSH-depleting agent, novel copper complex viz., N -(2-hydroxyacetophenone) glycinate (CuNG) has been synthesized, which was initially found to be potential resistance modifying agent and later an immunomodulator in mice model different doses. The objective the present work decipher effect CuNG on reactive oxygen species (ROS) generation antioxidant enzymes normal doxorubicin-resistant Ehrlich ascites carcinoma (EAC/Dox)-bearing Swiss albino mice....
T regulatory cells (Treg) avert autoimmunity, but their increased levels in melanoma confer a poor prognosis. To explore the basis for Treg accumulation melanoma, we evaluated chemokine expression patients. A 5-fold increase was documented chemoattractants CCL22 and CCL1 melanoma-affected skin versus unaffected skin, as accompanied by infiltrating FoxP3+ cells. In parallel, there an approximately two-fold enhancement of CCR4 circulating not effector We hypothesized that redirecting away from...
The efficacy of photodynamic treatment (PDT) against deep-seated tumor is hindered by low penetration depth light as well hypoxic conditions which prevails in tumor. To overcome this limitation, Near-infrared (NIR) absorbing photosensitizers have been investigated actively. In the present study we evaluated PDT an NIR chlorophyll derivative 'Cycloimide Purpurin-18 (CIPp-18)' Human Breast carcinoma (MCF-7) and cervical adenocarcinoma (Hela) cells under normoxic conditions. with CIPp-18 (2.0...
In the tumor microenvironment (TME), regulatory T cells (T regs ) adapt their metabolism to thrive in low-glucose, high-lactate conditions, but mechanisms remain unclear. Our study identifies CD38 as a key regulator of this adaptation by depleting nicotinamide adenine dinucleotide (oxidized form) (NAD + ), redirecting lactate-derived pyruvate toward phosphoenolpyruvate and bypassing tricarboxylic acid (TCA) cycle. This prevents accumulation α-ketoglutarate, which destabilizes inducing...
IL-17 producing CD4(+) T cells (Th17) are identified as a subset of proinflammatory present at the tumor site various murine and human cancer cases plays crucial role in shaping neoplastic process through fostering angiogenesis metastasis. However, development Th17 response microenvironment has not yet been fully elucidated. Herein, we make an attempt to disclose involvement infiltrating antigen presenting (APCs), especially associated macrophages (TAMs) myeloid derived suppressor (MDSCs)...
Background Multi drug resistance (MDR) or cross-resistance to multiple classes of chemotherapeutic agents is a major obstacle successful application chemotherapy and basic problem in cancer biology. The multidrug gene, MDR1, its gene product P-glycoprotein (P-gp) are an important determinant MDR. Therefore, there urgent need for development novel compounds that not substrates effective against drug-resistant cancer. Methodology/Principal Findings In this present study, we have synthesized...
PIM kinase family members play a crucial role in promoting cell survival and proliferation via phosphorylation of their target substrates. In this study, we investigated the kinases with respect to T responses transplantation tumor immunity. We found that PIM-2 isoform negatively regulated alloantigen, contrast PIM-1 PIM-3 isoforms, which acted as positive regulators. cells deficient demonstrated increased differentiation toward Th1 subset, proliferation, migration organs after allogeneic...
At the early stages of carcinogenesis, induction tumor specific T cell mediated immunity seems to block growth and give protective anti-tumor immune response. However, associated macrophages (TAMs) might play an immunosuppressive role subvert this anti leading progression metastasis.The Cu (II) complex, (chelate), copper N-(2-hydroxy acetophenone) glycinate (CuNG), synthesized by us, has previously been shown have a potential usefulness in immunotherapy multiple drug resistant cancers. The...