Importance Given conflicting results regarding the prognosis of erb-b2 receptor tyrosine kinase 2 (ERBB2; formerly HER2 or HER2/neu)–low breast cancer, a large-scale, nationally applicable comparison ERBB2-low vs ERBB2-negative cancer is needed. Objective To investigate whether clinically distinct subtype in terms epidemiological characteristics, prognosis, and response to neoadjuvant chemotherapy. Design/Participants/Setting This retrospective cohort study was conducted using National...

Abstract Diets containing either freeze-dried black raspberries (BRBs) or their polyphenolic anthocyanins (ACs) have been shown to inhibit the development of N-nitrosomethylbenzylamine (NMBA)–induced esophageal cancer in rats. The present study was conducted determine whether PCA, a major microbial metabolite raspberry (BRB) ACs, also prevents NMBA-induced F344 rats were injected with NMBA three times week for 5 weeks and then fed control experimental diets 6.1% BRBs, an anthocyanin...

Abstract Freeze-dried black raspberries (BRB), their component anthocyanins (AC), and a metabolite of BRB ACs, protocatechuic acid (PCA), inhibit the development esophageal cancer in rats induced by carcinogen, N-nitrosomethylbenzylamine (NMBA). All three components reduce inflammation esophagus plasma. The present study determined relation changes inflammatory markers to infiltration innate immune cells into NMBA-treated esophagus. Rats were injected with NMBA (0.35 mg/kg) for 5 weeks while...

Purpose: HER2-positive breast cancer is driven by cells possessing stem-like properties of self-renewal and differentiation, referred to as stem (CSC). CSCs are implicated in radiotherapy, chemotherapy resistance, tumor recurrence. NOTCH promotes CSC survival self-renewal, overexpression NOTCH1 the ligand JAGGED1 predict poor outcome. Resistance anti-HER2 therapy HER2+ requires NOTCH1, that combination trastuzumab a gamma secretase inhibitor (GSI) prevents relapse xenograft...

Abstract Estrogen receptor (ER)-positive breast cancer recurrence is thought to be driven by tumor-initiating cells (TIC). TICs are enriched endocrine therapy through NOTCH signaling. Side effects have limited clinical trial testing of NOTCH-targeted therapies. Death-associated factor 6 (DAXX) a newly identified marker whose RNA expression inversely correlates with in human ER+ tumor samples. In this study, knockdown and overexpression approaches were used investigate the role DAXX on...

BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is one of two subtypes esophageal cancer, with high incidence and mortality rates in developing countries. OBJECTIVE: The current study investigated the potential chemoprotective effects strawberries aspirin against development rat papillomas, precursors to ESCC. METHODS: Using a prevention model, we administered diets rats before, during, after N-nitrosomethylbenzylamine (NMBA) treatment. four were evaluated: control diet, 5% strawberry...

Abstract Recurrence of estrogen receptor (ER)-positive breast tumors despite curative-intent adjuvant therapy is thought to be due enrichment tumor initiating cells (TIC) during endocrine (ET). Recently, it was identified that by antagonizing the ER, ET promotes rapid degradation death-associated factor 6 (DAXX) protein, which necessary and sufficient potently inhibit TICs. Thus, goal current study identify a DAXX-inducing agent TICs prevent proliferation tumor. Phytoestrogens (naringenin,...

Black raspberries (BRB) have been shown to inhibit the progression of a number cancers including breast, esophageal, colon, and oral both in preclinical models.These results led further clinical evaluation for use BRB prevention cancer onset high risk populations as well preventing its progression.However, therapy can be challenging due variability nutritional content lots differences patient metabolism active components.Thus, preparation fractions possessing enriched amounts constituents...

Abstract Title: Epidemiology and Prognosis of HER2-Low Breast Cancer (BC) in the National Data Base (NCDB) Background: Characterization HER2 status has focused on overexpressing BC, which are uniquely sensitive to HER2-directed therapy. However, approximately 60% BC traditionally characterized as HER2-negative express low levels immunohistochemistry (IHC). Although these ‘HER2-low’ cancers insensitive traditional blockade, a recent randomized clinical trial showed that antibody drug...

Abstract Black raspberries (BRB) and their components have been demonstrated to prevent inhibit the progression of cancers including oral, breast, esophageal, colon in both preclinical studies human clinical trials. The mechanism for anticancer effect appears vary by organ system, include anti‐inflammatory, antiproliferative, antiangiogenic, pro‐apoptotic properties. Given findings that suggest host microbiome plays a distinct role development cancer, there has recent focus on discerning if...

Abstract Objectives Tumor-immune interactions are a critical determinant of tumor evolution and increasingly targeted by novel therapeutics (e.g. immune checkpoint blockade). However, their significance in treatment-naive localized prostate cancer remains largely unknown. Here, we employ multiplexed tissue imaging on radical prostatectomy specimens across Gleason grades to create single-cell spatial atlas primary tumors characterize innate adaptive landscape. Methods Whole-slide (RP) were...

Abstract Black raspberries (BRBs) a natural food demonstrated to have anti-oxidant, anti-inflammatory and anti-cancer activities,have been shown inhibit oral, esophageal, mammary gland colon cancers in rodents. Several human trials completed date assess the efficacy of BRB formulations for cancer prevention. However, chemopreventive potential BRBs against prostate cancer, most commonly diagnosed malignancy second leading cause death among men United States, is yet be reported. Among murine...

<p>Additional Materials and Methods</p>

<p>Figure S1: Individual DAXX siRNA sequences have similar effect on expression and TIC-survival. Figure S2: status has little bulk cell proliferation. S3: Survival curves of each dilution from ELDA. S4: Kaplan-Meier RFS Outcomes. S5: Outcomes based Nodal status. S6: Global changes in gene due to depletion. S7: Sites enrichment resulting EMT following knockdown. S8: DNA methylation or DNMT1 is required for restriction TIC-survival expression. S9: requires restrict TIC-associated...

<p>PCR and ChIP Primer Sequences KMplotter criteria</p>

<p>Additional Materials and Methods</p>

<p>Figure S1: Individual DAXX siRNA sequences have similar effect on expression and TIC-survival. Figure S2: status has little bulk cell proliferation. S3: Survival curves of each dilution from ELDA. S4: Kaplan-Meier RFS Outcomes. S5: Outcomes based Nodal status. S6: Global changes in gene due to depletion. S7: Sites enrichment resulting EMT following knockdown. S8: DNA methylation or DNMT1 is required for restriction TIC-survival expression. S9: requires restrict TIC-associated...

<p>Lapatinib induces Jagged1 transcripts but decreases total protein expression.</p>

<p>Expression of CD44 and CD24 in response to lapatinib.</p>

<p>Primer Sequences for RT-PCR</p>