A5070575027- Plant Molecular Biology Research
- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Fermentation and Sensory Analysis
- Plant Pathogens and Resistance
- GABA and Rice Research
- Biochemical and biochemical processes
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Horticultural and Viticultural Research
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant biochemistry and biosynthesis
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Wheat and Barley Genetics and Pathology
Institut National Polytechnique de Toulouse
2005-2025
Laboratoire de Recherche en Sciences Végétales
2021-2025
Genomics and Biotechnology of the Fruits Laboratory
2009-2025
Université de Toulouse
2009-2025
Université Toulouse III - Paul Sabatier
2022-2025
Centre National de la Recherche Scientifique
1994-2025
École Nationale Supérieure Agronomique de Toulouse
2003-2015
Génétique Physiologie et Systèmes d'Elevage
2009
Institut National de la Recherche Agronomique
2008
Agropolis International
2000-2002
Abstract Auxin/indole-3-acetic acid (Aux/IAA) proteins are transcriptional regulators that mediate many aspects of plant responses to auxin. While functions most Aux/IAAs have been defined mainly by gain-of-function mutant alleles in Arabidopsis thaliana, phenotypes associated with loss-of-function mutations scarce and subtle. We report here the downregulation IAA9, a tomato (Solanum lycopersicum) gene from distinct subfamily Aux/IAA genes, results pleiotropic phenotype, consistent its...
Rice was chosen as a model organism for genome sequencing because of its economic importance, small size, and syntenic relationship with other cereal species. We have constructed bacterial artificial chromosome fingerprint-based physical map the rice to facilitate whole-genome rice. Most ( approximately 90.6%) anchored genetically by overgo hybridization, DNA gel blot in silico anchoring. Genome data also were integrated into map. Comparison genetic maps reveals that recombination is...
Summary A library of 29 482 T‐DNA enhancer trap lines has been generated in rice cv. Nipponbare. The regions flanking the left border from first 12 707 primary transformants were systematically isolated by adapter anchor PCR and sequenced. survey 7480 genomic sequences larger than 30 bp (average length 250 bp), representing 56.4% total readable matching bacterial artificial chromosome/phage chromosome (BAC/PAC) assembled pseudomolecules allowed assigning 6645 (88.8%) insertion sites to at...
Four new members of the ERF (ethylene‐response factor) family plant‐specific DNA‐binding (GCC box) factors were isolated from tomato fruit ( LeERF1–4 ). Phylogenetic analysis indicated that LeERF2 belongs to a class, characterized by conserved N‐terminal signature sequence. Expression patterns and cis/trans binding affinities differed between LeERFs. Combining experimental data modeled three‐dimensional analysis, it was shown affinity LeERFs affected both variation nucleotides surrounding...
Auxin is a central hormone that exerts pleiotropic effects on plant growth including the development of roots, shoots, flowers and fruit. The perception signaling auxin rely cooperative action several components, among which auxin/indole-3-acetic acid (Aux/IAA) proteins play pivotal role. In this study, we identified comprehensively analyzed entire Aux/IAA gene family in tomato (Solanum lycopersicum), reference species for Solanaceae plants, model fleshy fruit development. Functional...
Abstract Background The phytohormone ethylene is involved in a wide range of developmental processes and mediating plant responses to biotic abiotic stresses. Ethylene signalling acts via linear transduction pathway leading the activation Response Factor genes ( ERF ) which represent one largest gene families transcription factors. How an apparently simple can account for complex widely diverse remains yet unanswered question. Building on recent release complete tomato genome sequence,...
Summary Fruit ripening involves a complex interplay between ethylene and ripening‐associated transcriptional regulators. E thylene R esponse F actors ( ERF s) are downstream components of signaling, known to regulate the expression ethylene‐responsive genes. Although fruit is an ethylene‐regulated process, role s remains poorly understood. The S l‐ . B 3 in tomato olanum lycopersicum ) maturation addressed here using chimeric dominant repressor version 3‐ SRDX ). Over‐expression results...
Ethylene response factors (ERFs) are plant transcriptional regulators mediating ethylene-dependent gene expression via binding to the GCC motif found in promoter region of ethylene-regulated genes. We report here on structural and functional characterization tomato Sl-ERF2 that belongs a distinct class large ERF family. Both spliced unspliced versions transcripts were amplified from RNA samples search public expressed sequence tag (EST) database confirmed existence two transcript species...
Pathogen attack represents a major problem for viticulture and agriculture in general. At present, the use of phytochemicals is more restrictive, therefore it becoming essential to control disease by having thorough knowledge resistance mechanisms. The present work focused on trans-regulatory proteins potentially involved plant defence response, WRKY proteins. A full-length cDNA, designated VvWRKY1, was isolated from grape berry library (Vitis vinifera L. cv. Cabernet Sauvignon). It encodes...
The hormone ethylene regulates a wide range of plant developmental processes and EBF (EIN3-binding F-box) proteins were shown to negatively regulate the signalling pathway via mediating degradation EIN3/EIL proteins. present study reports on identification two tomato F-box genes, Sl-EBF1 Sl-EBF2 from subfamily. genes display contrasting expression patterns in reproductive vegetative tissues response auxin treatment. are actively regulated at crucial stages development organs. Their dynamic...
The genome of tomato ( Solanum lycopersicum L.) is being sequenced by an international consortium 10 countries (Korea, China, the United Kingdom, India, Netherlands, France, Japan, Spain, Italy, and States) as part larger “International Solanaceae Genome Project (SOL): Systems Approach to Diversity Adaptation” initiative. sequencing project uses ordered bacterial artificial chromosome (BAC) approach generate a high‐quality euchromatic sequence for use reference euasterids. Sequence deposited...
A cDNA clone encoding a small GTP‐binding protein, the ADP‐ribosylation factor (ARF) was isolated from library of Arabidopsis thaliana cultured cells. The predicted amino acid sequence highly homologous to known yeast, bovine and human ARF sequences. Southern analysis genomic DNA suggested existence at least two copies genes. level mRNA found be nearly constant during all cell growth stages in suspension cultures.
Summary Ethylene R esponse F actors ( ERF s) are downstream components of the ethylene signal transduction pathway, although their role in ethylene‐dependent developmental processes remains poorly understood. As ethylene‐inducible tomato S l‐ . B 3 has been shown previously to display a strong binding affinity GCC ‐box‐containing promoters, its physiological significance was addressed here by reverse genetics approach. However, classical up‐ and down‐regulation strategies failed give clear...
Abstract Ripening is the last stage of developmental program in fleshy fruits. During this phase, fruits become edible and acquire their unique sensory qualities post-harvest potential. Although our knowledge mechanisms that regulate fruit ripening has improved considerably over past decades, processes trigger transition to remain poorly deciphered. While transcriptomic profiling tomato (Solanum lycopersicum L.) date mainly focused on changes occurring pericarp tissues between Mature Green...
Haplotype-resolved genome assemblies were produced for Chasselas and Ugni Blanc, two heterozygous Vitis vinifera cultivars by combining high-fidelity long-read sequencing high‐throughput chromosome conformation capture (Hi-C). The telomere-to-telomere full coverage of the chromosomes allowed us to assemble separately haplo-genomes both revealed structural variations between haplotypes a given cultivar. deletions/insertions, inversions, translocations, duplications provide insight into...
The transition from flower to fruit, naturally triggered by pollination and known as fruit set, is instrumental for plant reproduction, seed formation, crop yield. Notably, this developmental process can also proceed in the absence of fertilization, although it remains unclear whether pollination-dependent pollination-independent sets undergo similar transcriptomic reprogramming. Genome-wide profiling flower-to-fruit transition, either pollination-induced or auxin treatment, shows that both...
Abstract Fruit ripening is a sophisticatedly orchestrated developmental process, unique to plants, that results in major physiological and metabolic changes, ultimately leading fruit decay seed dispersal. Because of their strong impact on nutritional sensory qualities, the ripening-associated changes have been matter sustained investigation aiming at unravelling molecular genetic basis ripening. Tomato rapidly emerged as model choice for fleshy research wealth resources genomics tools...
Abstract Background Flavonoid pathway is spatially and temporally controlled during plant development the transcriptional regulation of structural genes mostly orchestrated by a ternary protein complex that involves three classes transcription factors (R2-R3-MYB, bHLH WDR). In grapevine ( Vitis vinifera L.), several MYB have been identified but interactions with their putative partners to regulate specific branches flavonoid are still poorly understood. Results this work, we describe effects...
Fruit development can be viewed as the succession of three main steps consisting fruit initiation, growth and ripening. These processes are orchestrated by different factors, notably successful fertilization flowers, environmental conditions hormones whose action is coordinated a large variety transcription factors. Among factor families, TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTOR (TCP) family has received little attention in frame biology despite its effects on several...
Ostreococcus tauri is a marine photosynthetic picoeukaryote presenting minimal cellular organization with one nucleus, chloroplast, and mitochondrion. It has the smallest genome described among free‐living eukaryotic cells, we showed by pulsed‐field gel electrophoresis (PFGE) that it divided between 15 bands ranging from 1.2 to 0.15 Mb, giving total size of 9.7 Mb. A Bacterial Artificial Chromosome (BAC) library was prepared genomic DNA extracted culture O. tauri. 2457 clones obtained an...