A5061678185- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Virology and Viral Diseases
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Ginseng Biological Effects and Applications
- Rabies epidemiology and control
- Animal Virus Infections Studies
- Vector-borne infectious diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Ziziphus Jujuba Studies and Applications
- Poxvirus research and outbreaks
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Biological and pharmacological studies of plants
- Neurological diseases and metabolism
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Prion Diseases and Protein Misfolding
- Biosensors and Analytical Detection
Animal and Plant Quarantine Agency
2014-2024
Ministry of Agriculture, Food and Rural Affairs
2013-2017
National Veterinary Research and Quarantine Service
2002-2011
Chungbuk National University
2004-2005
FAO/IAEA Agriculture and Biotechnology Laboratories
2005
Département Santé Animale
2005
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2005
The Pirbright Institute
2005
Iowa State University
2004
Korea Institute of Science and Technology
2000
African swine fever, a fatal haemorrhagic disease of swine, was confirmed in domestic pigs for the first time South Korea September 2019. The causative virus belonged to p72 genotype II and had an additional tandem repeat sequence intergenic region (IGR) between I73R I329L.
A seven-year-old male elk (Cervus elaphus nelsoni) was euthanized and necropsied after having a 3-week history of body weight loss, emaciation, excessive salivation, teeth grinding, fever, anorexia, respiratory distress. The imported into Korea from Canada on March 9, 1997. Gross pathologic findings were restricted to diffuse fibrinous pneumonia. Microscopic lesions included mild neuronal vacuolation spongiform change in the neuropil selected brain stem nuclei generalized astrocytosis....
The capsid protein of the West Nile virus (WNV) functions as an apoptotic agonist via induction mitochondrial dysfunction and activation caspases-9 -3. Here, we have determined that WNV (WNVCp) is capable binding to sequestering HDM2 into nucleolus. WNVCp was shown interfere with formation p53 complex, thereby causing stabilization subsequent its target protein, Bax. Whereas inducing p53-dependent process in wild-type mouse embryonic fibroblasts (MEF) or SH-SY5Y cells, it exerted no...
African swine fever (ASF) is a highly fatal viral disease affecting pigs. It caused by the ASF virus (ASFV), and causes serious economic losses to industry worldwide, including in Korea. Commercially available enzyme-linked immunosorbent assay (ELISA) kits for detecting anti-ASFV antibodies are used diagnosis surveillance of ASF. In this study, an ELISA was developed detect using two recombinant proteins, p22 p30, from genotype II ASFV. Recombinant transmembrane domain-deleted (p22∆TM) p30...
On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed South Korea. By 9 October, 14 outbreaks ASF domestic pigs had been diagnosed 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that fourteen ASFV isolates Korea belong to genotype II serogroup 8. Additionally, an intergenic region (IGR) variant with additional tandem repeat sequences (TRSs) between I73R I329L genes...
Recent global warming trends may have a significant impact on vector-borne viral diseases, possibly affecting vector population dynamics and disease transmission. This study measured levels of hemagglutination-inhibition (HI) antibodies against Japanese encephalitis virus (JEV) neutralizing Akabane (AKAV) Aino (AINV) forThoroughbred horses in Korea.Blood samples were collected from 989 racehorses several provinces, between October 2005 March 2007.Sera tested using either an HI assay or...
ABSTRACT Peste des petits ruminants (PPR) is a contagious viral disease of small that economic importance in Africa, the Middle East, and Asia. We developed rapid competitive enzyme-linked immunosorbent assay (rapid c-ELISA) for diagnosis surveillance PPR. This detects PPR virus (PPRV) antibodies serum samples by quantifying amount monoclonal antibody (MAb) P-3H12 after 30 min incubation serum-MAb conjugate mixture on plates coated with PPRV recombinant nucleocapsid protein (rPPRV-N). tested...
ABSTRACT Attempts were made to identify and map epitopes on the nucleocapsid (N) protein of peste des petits ruminants virus (PPRV) (Nigeria75/1 strain) using seven monoclonal antibodies (MAbs) deletion mutants. At least four antigenic domains (A-I, A-II, C-I, C-II) identified MAbs. Domains A-I (MAb 33-4) A-II (MAbs 38-4, P-3H12, P-13A9) determined be located amino-terminal half (amino acids [aa] 1 262), C-I (P-14C6) C-II (P-9H10 P-11A6) within carboxy-terminal region (aa 448 521)....
Rapid and specific detection of foot-and-mouth disease virus (FMDV) is a key factor for promoting prompt control FMD outbreaks. In this study, real-time reverse transcription loop-mediated isothermal amplification (RRT-LAMP) assay with high sensitivity, rapidity reliability was developed using targeted gene-specific assimilating probe seven FMDV serotypes. Positive signals were generated within 15 min the lowest concentration standard RNA sample at 62°C; substantially faster than that...
Rift Valley fever virus (RVFV) is one of the important emerging viral diseases serious impact in public health and animal hygiene both human industries. In this study, we developed a monoclonal antibody-based competitive ELISA for detection antibodies to RVFV goats cattle. The recombinant N protein was expressed E. coli with six-histidine tag, purified used detecting antigen antibody against antibodies. (C-ELISA) could detect at 9-11 days after inoculation cattle sensitivity 94.7% (virus...
ABSTRACT An inactivated SVDV antigen is used in current enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to swine vesicular disease virus (SVDV). To develop a noninfectious recombinant alternative, we produced SVDV-like particles (VLPs) morphologically and antigenically resembling authentic by using dual baculovirus recombinant, which expresses simultaneously P1 3CD protein genes under different promoters. Antigenic differences between VLPs were not statistically...
Velogenic Newcastle disease virus (NDV) was recovered from two dead Eurasian Scops Owls (Otus scops) a wildlife rescue center in Korea during 2005. Phylogenetic analysis based on the sequence of partial fusion (F) protein revealed that isolates had highest level homology to recent Korean NDV strains poultry.
Purpose: New alternative bait rabies vaccines applicable to pet dogs and wild animals are needed eradicate in Korea.In this study, recombinant virus, ERAG3G strain was constructed using reverse genetic system the safety, efficacy immunogenicity of evaluated mice dogs.Materials Methods: Using full-length genome mutated amino acid at position 333 glycoprotein virus (RABV) helper plasmids, rescued BHK/T7-9 cells successfully.Mice were inoculated with for safety efficacy.Safety dog (1 mL, 10 8.0...
Rabies is an important viral zoonosis that causes acute encephalitis and death in mammals. To date, several recombinant vaccines have been developed based on G protein, which considered to be the main antigen, these are used for rabies control many countries. Most viruses expressing RABV protein retain gene from attenuated RABV. Not enough currently known about protective effect against of a combination adenoviruses N proteins pathogenic street We constructed adenovirus (Ad-0910Gsped) signal...
Purpose: Japanese encephalitis is a reproductive disorder caused by virus (JEV) in swine.Recent genotype (G) shift phenomenon (G3 to G1) the Asia-wide has posed challenge for proper prevention current vaccine strain.Thus, new kinds of JEV G1 vaccines with enhanced immunogenicity have been required pigs.Materials and Methods: Recombinant porcine granulocyte monocyte-colony stimulating factor (reporGM-CSF) protein was expressed Spodoptera frugiperda (Sf-9) cells using baculovirus expression...
Foot-and-mouth disease (FMD) is a highly contagious and economically devastating affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A one of the most common causes outbreaks among seven FMDV serotypes, its serological diagnosis therefore important to confirm infection determine vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific via hybridoma systems using an inactivated (A22/Iraq/1964) found 4 monoclones (#29, #106, #108, #109) with high binding...