A5055849392- Prion Diseases and Protein Misfolding
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Neurological diseases and metabolism
- Trace Elements in Health
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Microbial infections and disease research
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Fungal and yeast genetics research
- Identification and Quantification in Food
- Parasite Biology and Host Interactions
- Viral Infections and Outbreaks Research
- Genomics and Phylogenetic Studies
- Mosquito-borne diseases and control
- RNA regulation and disease
- HIV Research and Treatment
Animal and Plant Quarantine Agency
2016-2025
Ministry of Agriculture, Food and Rural Affairs
2023
Colorado State University
2012-2022
University of Kentucky
2010-2022
Seoul National University
2012
Bial (Portugal)
2010
Korea Research Institute of Bioscience and Biotechnology
2002-2003
Prions are infectious proteins composed of the abnormal disease-causing isoform PrPSc, which induces conformational conversion host-encoded normal cellular prion protein PrPC to additional PrPSc. The mechanism underlying strain mutation in absence nucleic acids remains unresolved. Additionally, frequency strains causing chronic wasting disease (CWD), a burgeoning epidemic cervids, is unknown. Using susceptible transgenic mice, we identified two prevalent CWD with divergent biological...
African swine fever, a fatal haemorrhagic disease of swine, was confirmed in domestic pigs for the first time South Korea September 2019. The causative virus belonged to p72 genotype II and had an additional tandem repeat sequence intergenic region (IGR) between I73R I329L.
Significance Searching for drugs to prevent conversion of host-encoded prion protein (PrP C ) its infectious conformation Sc is a key strategy in the pursuit therapies disorders: fatal, transmissible epidemic diseases unpredictable occurrence and uncertain zoonotic potential. Despite quinacrine’s ability reduce mouse PrP cell models, use treat patients has been unsuccessful. Here, we show that quinacrine augments intensifies replication prions, causing chronic wasting disease deer, elk,...
The epitope of the 3F4 antibody most commonly used in human prion disease diagnosis is believed to consist residues Met-Lys-His-Met (MKHM) corresponding PrP-(109-112). This assumption based mainly on observation that reacts with and hamster PrP but not from mouse, sheep, cervids, which Met at residue 112 replaced by Val. Here we report that, brain histoblotting, did react uninfected transgenic mice expressing elk PrP; however, it show distinct immunoreactivity infected chronic wasting...
Understanding the molecular parameters governing prion propagation is crucial for controlling these lethal, proteinaceous, and infectious neurodegenerative diseases. To explore effects of protein (PrP) sequence structural variations on intra- interspecies transmission, we integrated studies in deer, a species naturally susceptible to chronic wasting disease (CWD), burgeoning, contagious epidemic uncertain origin zoonotic potential, with transgenic (Tg) mouse modeling cell-free amplification....
Mesenchymal stem cells (MSCs) are often known to have a therapeutic potential in the cell-mediated repair for fatal or incurable diseases. In this study, canine umbilical cord MSCs (cUC-MSCs) were isolated from matrix (n = 3) and subjected proliferative culture 5 consecutive passages. The at each passage characterized multipotent MSC properties such as proliferation kinetics, expression patterns of surface markers self-renewal associated markers, chondrogenic differentiation. results,...
African swine fever (ASF) is a highly fatal viral disease affecting pigs. It caused by the ASF virus (ASFV), and causes serious economic losses to industry worldwide, including in Korea. Commercially available enzyme-linked immunosorbent assay (ELISA) kits for detecting anti-ASFV antibodies are used diagnosis surveillance of ASF. In this study, an ELISA was developed detect using two recombinant proteins, p22 p30, from genotype II ASFV. Recombinant transmembrane domain-deleted (p22∆TM) p30...
Abstract Background Chronic wasting disease (CWD) in North America is an ineradicable and deadly infectious neurodegenerative disorder of free-ranging captive cervids caused by prions. While CWD was inadvertently introduced to South Korea (SK) following importation sub-clinically diseased elk from America, it unclear whether this event represented a bottleneck infection uncommon prion strain and/or if variant strains evolved during subsequent transmissions additional farmed cervid species...
On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed South Korea. By 9 October, 14 outbreaks ASF domestic pigs had been diagnosed 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that fourteen ASFV isolates Korea belong to genotype II serogroup 8. Additionally, an intergenic region (IGR) variant with additional tandem repeat sequences (TRSs) between I73R I329L genes...
African swine fever virus (ASFV) genotype II has been circulating in South Korea, causing substantial economic losses to the Korean pig industry since 2019. Genetic epidemiological investigations using whole-genome sequencing have conducted track genetic evolution of ASFV. Two ASFV strains were detected domestic farms one with a large deletion MGF 360-6L gene and other 360-21R gene. Phylogenetic analysis indicated that all isolates belonged Asian subgroup further divided into distinct...
Whereas prion replication involves structural rearrangement of cellular protein (PrP(C)), the existence conformational epitopes remains speculative and controversial, PrP transformation is monitored by immunoblot detection PrP(27-30), a protease-resistant counterpart pathogenic scrapie form (PrP(Sc)) PrP. We now describe involvement specific amino acids in determinants novel monoclonal antibodies (mAbs) raised against randomly chimeric Epitope recognition two mAbs depended on polymorphisms...
Since the first African swine fever (ASF) outbreak occurred at a pig farm in South Korea September 2019, as of 31 January 2023, ASF cases have farms, while 2799 virus (ASFV)-infected wild boars been identified. The circulation ASFV boar populations poses high risk spillover to farms country. However, information on changes pathogenicity Korean strains from is not available. Investigating only way predict their alterations. In previous study, no circulating during 2019–2021 were identified...
The glyceraldehyde-3-phosphate dehydrogenase promoter, P(GAP), was employed to direct the constitutive expression of recombinant human serum albumin (HSA) in Hansenula polymorpha. A set integration vectors containing HSA cDNA under control P(GAP) constructed and elemental parameters affecting from were analyzed. presence a 5'-untranslated region derived vector into GAP locus shown improve P(GAP). Glycerol supported higher level along with cell density than either glucose or methanol. growth...
Although they share certain biological properties with nucleic acid based infectious agents, prions, the causative agents of invariably fatal, transmissible neurodegenerative disorders such as bovine spongiform encephalopathy, sheep scrapie, and human Creutzfeldt Jakob disease, propagate by conformational templating host encoded proteins. Once thought to be unique these diseases, this mechanism is now recognized a ubiquitous means information transfer in systems, including other protein...
Conformational conversion of the normal cellular isoform prion protein PrP
The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during infection remain undetermined, in part because a lack monoclonal antibodies (mAbs) that can distinguish these isoforms. Here we show the anti-PrP mAb PRC7 recognizes an epitope is shielded from detection when glycans are attached to Asn-196. We observed whereas PrPC predisposed full glycosylation and therefore refractory detection, leads diminished PrPSc...
African swine fever (ASF) is a fatal contagious disease affecting swine. The first Korean ASF virus (ASFV) isolate (Korea/Pig/Paju1/2019) was used to compare the course of ASFV in pigs inoculated via four routes. In challenge experiment, domestic were infected intraoral (IO) and intranasal (IN) routes with 106 50% hemadsorbing dose (HAD50) an intramuscular (IM) injection 103 HAD50. direct contact (DC) group, five naïve brought into two IM-ASFV-infected pigs. IO-, IN-, IM-inoculated showed...
African swine fever (ASF) was first reported in South Korea September 2019, and as of 31 December 2021, a total 21 cases domestic pig farms 1875 ASFV-infected wild boars have been confirmed the country. With continued circulation ASF boars, subsequent outbreaks pigs, concerns were raised about possible changes virulence occurring among viruses (ASFV) circulating Korea. In this study, four Korean ASFV strains isolated from at different time points between 2019 2021 chosen, used to...