A5069639073- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Polymer Surface Interaction Studies
- Conducting polymers and applications
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Click Chemistry and Applications
- Bacterial Identification and Susceptibility Testing
- Single-cell and spatial transcriptomics
- Genomic variations and chromosomal abnormalities
- Neuroscience and Neural Engineering
- Epigenetics and DNA Methylation
- Respiratory viral infections research
- Chromosomal and Genetic Variations
Korea Advanced Institute of Science and Technology
2014-2024
Korea Research Institute of Bioscience and Biotechnology
2022-2024
Yonsei University
2023
University of Seoul
2022
Korea University
2022
Korea University of Science and Technology
2022
Government of the Republic of Korea
2017-2019
Kootenay Association for Science & Technology
2017-2019
Daejeon University
2017-2019
Abstract Development of coating technologies for electrochemical sensors that consistently exhibit antifouling activities in diverse and complex biological environments over extended time is vital effective medical devices diagnostics. Here, we describe a micrometer-thick, porous nanocomposite with both electroconducting properties enhances the sensitivity sensors. Nozzle printing oil-in-water emulsion used to create 1 micrometer thick composed cross-linked albumin interconnected pores gold...
Coronavirus disease (COVID-19) has affected people for over two years. Moreover, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants raised concerns regarding its accurate diagnosis. Here, we report a colorimetric DNAzyme reaction triggered by loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR), referred to as DAMPR assay detecting SARS-CoV-2 and genes attomolar sensitivity within an hour....
Since COVID-19 and flu have similar symptoms, they are difficult to distinguish without an accurate diagnosis. Therefore, it is critical quickly accurately determine which virus was infected take appropriate treatments when a person has infection. This study developed dual-mode surface-enhanced Raman scattering (SERS)-based LFA strip that can diagnose SARS-CoV-2 influenza A with high accuracy reduce the false-negative problem of commercial colorimetric strip. Furthermore, using single strip,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed the detection of SARS-CoV-2, advanced sensing methods are required diagnosis, screening, and surveillance disease 2019 (COVID-19). Here, we report surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), magnetic beads SARS-CoV-2. The selected pair against SARS-CoV-2...
Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate sensitive diagnosis of infectious diseases, especially those with low levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip enrichment/isolation. This features unique nanostructures comprising large small pore layers. Computational simulations confirm that these enhance the surface area promote formation nanovortex, resulting in improved capture efficiency. Notably,...
Abstract Endonucleases have recently widely used in molecular diagnostics. Here, we report a strategy to exploit the properties of Argonaute (Ago) proteins for diagnostics by introducing an artificial nucleic acid circuit with Ago protein (ANCA) method. The ANCA is designed perform continuous autocatalytic reaction through cross-catalytic cleavage protein, enabling one-step, amplification-free, and isothermal DNA detection. Using method, carbapenemase-producing Klebsiella pneumoniae (CPKP)...
The escalating global threat of infectious diseases, including monkeypox virus (MPXV), necessitates advancements in point-of-care diagnostics, moving beyond the constraints conventional methods tethered to centralized laboratories. Here, we introduce multiple CRISPR RNA (crRNA)-based biosensors that can directly detect MPXV within 35 minutes without pre-amplification, leveraging enhanced sensitivity and antifouling attributes BSA-based nanocomposite. Multiple crRNAs, strategically targeting...
We herein describe a label-free and enzyme-free signal amplification strategy for the sensitive determination of ribonuclease H (RNase H) activity, which relies on target-triggered catalytic hairpin assembly (CHA) in conjunction with G-quadruplex specific fluorescent binder, N-methyl mesoporphyrin IX (NMM). In absence RNase H, RNA/DNA duplex serving as substrate cannot initiate execution CHA that produces G-quadruplexes; so NMM shows low fluorescence signal. contrast, presence degrades RNA...
Accurate and efficient detection of DNA is crucial for disease diagnosis health monitoring. The traditional methods analysis involve multiple steps, including sample preparation, lysis, extraction, amplification, detection. In this study, we present a one-step elution-free method based on the combination clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated light-up aptamer transcription (CLAT) assay DNA-capturing...
We have devised a barcode DNA-mediated signal amplifying strategy for ultrasensitive biomolecular detection by utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). As model target, thrombin was first captured specific aptamer15 functionalized on magnetic beads (MBs-apt15) and sandwiched through the simultaneous interaction with gold nanoparticles modified another aptamer29 DNA molecules (apt29-AuNPs-bcDNAs). The complex collected convenient...
• The 3D interior organo-hotspots was demonstrated via electrochemical deposition onto Au nanodimple platforms with viral lysates. proposed platform exhibited the label-free SERS detection of SARS-CoV-2 traced real clinical samples in a sensitive, quantitative, and reliable manner. In recent decades, biomedical sensors based on surface-enhanced Raman spectroscopy (SERS), which reveals unique spectral features corresponding to individual molecular vibrational states, have attracted intensive...
Digital polymerase chain reaction (dPCR) is a promising alternative for achieving absolute quantification in molecular diagnostics. To improve its effectiveness, it necessary to develop comprehensive device that not only optimizes the partitioning process but also integrates microdroplet generation and transfer. Herein, we report an advanced microfluidic generating uniform gene-encapsulated microdroplets multiplex droplet-based dPCR (ddPCR). The consists of eight repeat units, each unit has...
A new isothermal nucleic acid amplification method termed FERA (Flap endonuclease-initiated Enzymatic Repairing Amplification) is developed for the ultrasensitive detection of target acids.
In this study, we uncover a ligation-free DNA extension method in two adjacent fragmented probes, which are hybridized to target RNA, for developing nucleic acid amplification reaction. reaction, elongation occurs from forward probe phosphorothioated-hairpin the presence of RNA regardless ligation. The second then simultaneously at nick site phosphorothioated and self-priming region. Therefore, binding clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated...
Abstract A nanoplasmonic biosensor for the precise detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) by leveraging advances in nanostructured sensing materials and highly selective monoclonal antibodies is presented. The sensor integrates plasmonic Au nanostructures optimized surface‐enhanced Raman scattering (SERS), enhancing sensitivity through unique light interactions at nanoscale. Coupled with exclusive that specifically target SARS‐CoV‐2 its evolving variants,...
A novel approach is introduced using nanoplasmonic microarray–based solid-phase recombinase polymerase amplification (RPA) that offers high sensitivity and multiplexing capabilities for gene detection. Nanoplasmonic microarrays were developed through one-step immobilization of streptavidin/biotin primers fine-tuning the amplicon size to achieve plasmon-enhanced fluorescence (PEF) on substrate, thereby improving sensitivity. The specificity RPA was evaluated in detecting E, N, RdRP genes...
Organic thin-film transistors (OTFTs) fabricated on a biocompatible Parylene-C substrate can be applied to biosensors using simple and cost-effective process. In this study, we developed OTFTs by organic materials fabricate the substrate, gate dielectric, channel, passivation layer. Poly(3-hexylthiophene) (P3HT) was used Parylene-C-based platform. As showed promising insulation properties. Finally, generated cyclic olefin polymer (COP) layer protect P3HT from oxygen moisture, effect of COP...
By harnessing its charge-shifting property, the polyplex-assisted qRT-PCR technique achieves efficient RNA capture and release. This innovation streamlines extraction & amplification in a one-pot process, offering rapid, user-friendly molecular diagnostics.
Abstract The current standard method of diagnosing coronavirus disease 2019 (COVID-19) involves uncomfortable and invasive nasopharyngeal (NP) sampling using cotton swabs (CS), which can be unsuitable for self-testing. Although mid-turbinate is an alternative, it has a lower diagnostic yield than NP sampling. Nasal wash (NW) similar to sampling, but cumbersome perform. In this study, we introduce 3D printed fluidic swab (3DPFS) that enables easy NW COVID-19 testing with improved yield. 3DPFS...