Mammalian cells cultured on 2D surfaces in microfluidic channels are increasingly used drug development and biological research applications. These systems would have more or clinical relevance if the exhibit 3D phenotypes similar to vivo. We developed a channel based system that allows be perfusion-cultured by supporting them with adequate cell-cell cell-matrix interactions. The maximal interaction was achieved perfusion-seeding through an array of micropillars; interactions were...

We have developed a microfluidic 3D hepatocyte chip (3D HepaTox Chip) for in vitro drug toxicity testing to predict vivo hepatotoxicity. The Chip is based on multiplexed channels where microenvironment engineered each channel maintain the hepatocytes' synthetic and metabolic functions. allow simultaneous administration of multiple doses functional primary hepatocytes while an incorporated concentration gradient generator enables dose-dependent responses IC50 values 5 model drugs derived from...

We have developed a multi-channel 3D microfluidic cell culture system (multi-channel 3D-microFCCS) with compartmentalized microenvironments for potential application in human drug screening. To this end, the 3D-microFCCS was designed culturing different cellular aggregates simultaneously to mimic multiple organs body. Four types (C3A, A549, HK-2 and HPA) were chosen represent liver, lung, kidney adipose tissue, respectively. Cellular functions optimized by supplementing common medium growth...

Starting gate: A molecular logic gate is based on chemosensors that change their fluorescence intensity in response to various inputs (pH, metal ions) a microfluidic system. combinatorial circuit, such as half adder (see picture), uses fluorescein (green) and rhodamine B (red) derivatives respond pH. with protein Cu2+ ions also described. Supporting information for this article available the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z703813_s.pdf or from author. Please note:...

We present a novel active mixing method in microfluidic chip, where the controlled stirring of magnetic particles is used to achieve an effective fluids. To perform mixing, ferromagnetic were embedded and manipulated under influence rotating field. By aligning beads along field lines, rod-like structures are formed, functioning as small stir bars. Under higher flow conditions did not form typical rod structure but rather formed aggregates, which even more beneficial for mixing. Our system...

We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues organs inside the embryo. The Fish Chips was micro-fabricated in silicon glass reproducibility accuracy structure. consists three parts: gradient generator, row eight fish tanks, which are individually placed, output channels. fluidic generator supports dose-dependent drug chemical studies. A unique system ensures uniform constant flow media to tank...

The combination of micropillar array technology to measure cellular traction forces with super-resolution imaging allowed us obtain force maps and simultaneously zoom in on individual focal adhesions single-molecule accuracy. We achieved a detection precision 500 pN mean localization 30 nm. Key the achievement was two-step etching process that provided an integrated spacer next permitted stable reproducible observation cells micropillars within short working distance high-magnification, high...

Most high-throughput screening (HTS) systems studying the cytotoxic effect of chimeric antigen receptor (CAR) T cells on tumor rely two-dimensional cell culture that does not recapitulate microenvironment (TME). Tumor spheroids, however, can TME and have been used for cytotoxicity assays CAR cells. But a major obstacle to use spheroids is difficulty in separating unbound dead from spheroids. Here, we present three-dimensional hanging spheroid plate (3DHSP), which facilitates formation...

Abstract With the introduction of microtechnology and microfluidic platforms for cell culture, stem research can be put into a new context. Inside microfluidics, microenvironments more precisely controlled their influence on fate studied. Microfluidic devices made transparent cells monitored real time by imaging, using fluorescence markers to probe functions fate. This article gives perspective yet untapped utility research. It will guide biologists through some basic application...

We have developed a microfluidic-based culture chip to simulate cancer cell migration and invasion across the basement membrane. In this microfluidic chip, 3D microenvironment is engineered metastatic breast cells (MX1) in tumor model. A chemo-attractant was incorporated stimulate motility validated usefulness of by tracking motilities system, showing them be migrating or invading (akin metastasis). It shown that our system can monitor real time, as compare Boyden chambers, for example....

The endocrine system is a collection of glands producing hormones which, among others, regulates metabolism, growth and development. One important group diseases diabetes, which caused by deficiency or diminished effectiveness endogenous insulin. By using microfluidic perfused 3D cell-culture chip, we developed an 'endocrine on chip' to potentially be able screen drugs for the treatment diabetes measuring insulin release over time. Insulin-secreting β-cells are located in pancreas, while...

Vergattert: Ein molekulares Logikgatter beruht auf Chemosensoren, die ihre Fluoreszenzintensität als Reaktion verschiedene Eingabeparameter (pH-Wert, Metallionen) in einem mikrofluidischen System ändern. Schaltnetz, z. B. ein Halbaddierer (siehe Bild), nutzt pH-empfindliche Fluorescein- (grün) und Rhodamin-B-Derivate (rot). mit Protein Cu2+-Ionen wird ebenfalls beschrieben.

Influenza A viruses are often present in environmental and clinical samples at concentrations below the limit of detection (LOD) molecular diagnostics. Here we report an integrated microfluidic preconcentration nucleic amplification system (μFPNAS) which enables both influenza virus H1N1 (H1N1) its viral RNA, thereby lowering LOD for H1N1. particles were first magnetically preconcentrated using magnetic nanoparticles conjugated with antibody specific virus. Their isolated RNA was amplified...

In vitro drug testing requires long-term maintenance of hepatocyte liver specific functions. Hepatocytes cultured at a higher seeding density in sandwich configuration exhibit an increased level functions when compared to low cultures due the better cell contacts that promote long term polarity and However, culturing hepatocytes high densities standard 24-well plate poses problems terms mass transport nutrients oxygen cells. view this drawback, we have developed polydimethylsiloxane (PDMS)...

Molecular diagnostics for sepsis is still a challenge due to the presence of compounds that interfere with gene amplification and bacteria at concentrations lower than limit detection (LOD). Here, we report on development 3D printed modular microfluidic device (3DpmμFD) preconcentrates interest in whole blood purifies their genomic DNA (gDNA). It composed W-shaped microchannel conical microchamber. Bacteria are magnetically captured from antibody conjugated magnetic nanoparticles (Ab-MNPs) 5...

In this study, we introduce a microfluidic device equipped with pneumatically actuated valves, generating linear gradient of chemoeffectors to quantify the chemotactic response Tetrahymena pyriformis, freshwater ciliate. The was fabricated from an elastomer, poly(dimethylsiloxane) (PDMS), using multi-layer soft lithography. components include electronically controlled pneumatic microvalves, microchannels and microchambers. established by releasing chemical ciliate-free microchamber into...

As paper-based diagnostics has become predominantly driven by more advanced microfluidic technology, many of the research efforts are still focused on developing reliable and versatile fluidic control devices, apart from improving sensitivity reproducibility. In this work, we introduce a novel robust paper system enabling control. The comprises linear push-pull solenoid an Arduino Uno microcontroller. precisely controlled pressure exerted stops flow. We first determined stroke distance to...